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Somatic cell alterations in interspecific hybrids of Cenchrus purpureum (Schumach.) and Cenchrus americanus (L.) Morrone by genomic in situ hybridization

Reis G.B., Mesquita A.T., Andradevieira L.F., Azevedo A.L.S., Davide L.C.


The cross between Cenchrus purpureum (Schumach.) (Napier grass) and Cenchrus americanus (L.) Morrone (pearl millet) produce triploid and sterile hybrids. Chromosome doubling used to restore the fertility of the triploid hybrids produces hexaploid material that are mixoploid, but with desirable forage characteristics. Even backcrosses result in tetraploid and pentaploid mixoploid materials. The aim of this study was to compare the frequency of chromosome abnormalities in somatic cells of hybrids originated by backcross to compare with those found in synthetic hexaploid hybrids finding if there is a most stable material. To identify the abnormalities it was used the genomic in situ hybridization. The cell cycle analysis revealed similar frequencies and types of alterations both in the hexaploid and in hybrids originating from backcrosses. Chromosome elimination was reported in all types of hybrids. It was found micronuclei, notoriented chromosomes at metaphase, lost chromosome at anaphase/telophase and bridges at anaphase involving chromosomes from Napier grass and pearl millet in all hybrids analyzed independently of ploidy level.

Key words: Pearl millet, Napier grass, chromosome alterations; chromosome elimination; micronucleus

Tsitologiya i Genetika 2021, vol. 55, no. 2, pp. 79-80

  1. Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brasil
  2. Departamento de Biologia Vegetal, Universidade Estadual de Campinas, Campinas, SP, Brasil
  3. Laboratório de Genética Vegetal, Embrapa Gado de Leite, Juiz de Fora, Juiz de Fora, MG, Brasil

E-mail: lfandrade.vieira

Reis G.B., Mesquita A.T., Andradevieira L.F., Azevedo A.L.S., Davide L.C. Somatic cell alterations in interspecific hybrids of Cenchrus purpureum (Schumach.) and Cenchrus americanus (L.) Morrone by genomic in situ hybridization, Tsitol Genet., 2021, vol. 55, no. 2, pp. 79-80.

In "Cytology and Genetics":
G. B. Reis, A. T. Mesquita, L. F. Andrade-Vieira, A. L. S. Azevedo & L. C. Davide Somatic Cell Alterations in Interespecific Hybrids of Cenchrus purpureum (Schumach.) and Cenchrus americanus (L.) Morrone by Genomic in Situ Hybridization, Cytol Genet., 2021, vol. 55, no. 2, pp. 194198
DOI: 10.3103/S0095452721020146


1. Abreu, J.C., Davide, L.C., Pereira, A.V., and Barbosa, S., Mixoploidia em híbridos de capim‑elefante x milheto tratados com agentes antimitóticos, Pesqui. Agropecu. Bras., 2006, vol. 41, pp. 16291635.

2. Andrade-Vieira, L.F., Reis, G.B., Torres, G.A., Oliveira, A.R., Brasileiro-Vidal, A.C., Pereira, A.V., and Davide, L.C., Biparental chromosome elimination in artificial interspecific hybrids Pennisetum purpureum and Pennisetum glaucum, Crop Sci., 2013, vol. 53, pp. 18.

3. Baptista-Giacomelli, F.R., Pagliarini, M.S., and Almeida, J.L.D., Elimination of micronuclei from microspores in a Brazilian oat (Avena sativa L.) variety, Genet. Mol. Biol., 2000, vol. 23, pp. 681684.

4. Campos, J.M.S., Davide, L.C., Salgado, C.C., Santos, F.C., Costa, P.N., Silva, P.S., Alves, C.C.S., Viccini, L.F., and Pereira, A.V., In vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucum, Plant Breed., 2009, vol. 128, pp. 101104.

5. Gupta, S.B., Duration of mitotic cycle and regulation of DNA replication in Nicotiana plumbaginifolia and hybrid derivative of N. tabacum showing chromosome instability, Can. J. Genet. Cytol., 1969, vol. 11, no. 1, pp. 133142.

6. Ishii, T., Toshie, U., Hiroyuki, T., and Hisashi, T., Chromosome elimination by wide hybridization between Triticeae or oat plant and pearl millet: pearl millet chromosome dynamics in hybrid embryo cells, Chromosome Res., 2010, vol.18, pp. 821831.

7. Jauhar, P.P., Cytogenetics and Breending of Pearl Millet and Related Species, New York: A. R. Liss, 1981.

8. Jauhar, P.P. and Hanna, W.W., Cytogenetics and genetics of pearl millet, Adv. Agronomy, 1998.

9. Jiang, J., Gill, B.S., Wang, G.L., Ronald, P.C., and Ward, D.C., Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes, Proc. Natl. Acad. Sci. U. S. A., 1995, vol. 92, pp. 44874491.

10. Koba, T., Handa, T., and Shimada, T., Efficient production of wheatbarley hybrids and preferential elimination of barley chromosomes, Theor. Appl. Gen., 1991, vol. 81, pp. 285292.

11. Leão, F.F., Cancellier, L.L., Pereira, A.V., Da Silva Ledo, F.J., and Afférri, F.S., Produção forrageira e composição bromatológica de combinações genômicas de capim-elefante e milheto, Revista Ciência Agronômica, 2012, vol. 43, pp. 368375.

12. Leão, F.F., Davide, L.C., Campos, J.M.S., Pereira, A.V., and Bustamante, F.O., Genomic behavior of hybrid combinations between elephant grass and pearl millet, Pesqui. Agropecu. Bras., 2011, vol. 46, pp. 712719.

13. Ma, X.F. and Gustafson, J.P., Genome evolution of allopolyploids: a process of cytological and genetic diploidization, Cytogenet. Genome Res., 2006, vol. 109, pp. 236249.

14. Martel, E., DeNay, D., Siljak Yakovlev, S., Brown, S., and Sarr, A., Genome size variation and basic chromosome number in pearl millet and fourteen related Pennisetum species, J. Hered., 1997, vol. 88, pp. 139143.

15. Ozkan, H., Tuna, M., and Arumuganathan, K., Nonadditive changes in genome size during allopolyploidization in the wheat (AegilopsTriticum) group, J. Hered., 2003, vol. 94, pp. 260264.

16. Paiva, E.A., Bustamante, F.O., Barbosa, S., Pereira, A.V., and Davide, L.C., Meiotic behavior in early and recent duplicated hexaploid hybrids of Napier grass (Pennisetum purpureum) and pearl millet (Pennisetum glaucum), Caryologia, 2012, vol. 65, pp. 114120.

17. Pereira, A.V., Valle, C.B., Ferreira, R.P., and Miles, J.W. Melhoramento de forrageiras tropicais, in Recursos Genéticos e Melhoramento de Plantas, Nass, L.L., Valois, A.C.C., Melo, I.S., and Valadares-Ingres, M.C., Eds., Rondonópolis: Fundação M.T., 2001, pp. 449601.

18. Pereira, A.V., Souza-Sobrinho, F., Souza, F.H.D., and Ledo, F.J.S., Tendências do melhoramento genético e produção de sementes forrageiras no Brasil, in Simpósio de Atualização eem Genética e Melhoramento de Plantas, 2003, 7, Lavras, Anais, Lavras: UFLA, 2003, pp. 3663.

19. Powell, F.D., Hanna, W., Anna, W., and Burton, G., Origin, cytology, and reproductive characteristic of haploids in pearl millet, Crop Sci., 1975, vol. 15, pp. 389392.

20. Reis, G.B., Mesquita, A.T., Torres, G.A., Andrade-Vieira, L.F., Pereira, A.V., and Davide, L.C., Genomic homeology between Pennisetum purpureum and Pennisetum glaucum (Poaceae), Comp. Cytogenet., 2014, vol. 8, pp. 199209.

21. Reis, G.B., Ishii, T., Fuchs, J., Houben, A., and Davide, L.C., Tissue-specific genome instability in synthetic interspecific hybrids of Pennisetum purpureum (Napier grass) and Pennisetum glaucum (pearl millet) is caused by micronucleation, Chromosome Res., 2016, vol. 12, pp. 13.

22. Sanei, M., Pickering, R., Fuchs, J., Banaei Moghaddam, A.M., Dziurlikowska, A., and Houben, A., Loss of centromeric histone H3 (CENH3) from centromeres precedes uniparental chromosome elimination in interspecific barley hybrids, Plant Biol., 2011, vol. 108, pp. 498505.

23. Santos, F.M.C., Torres, G.A., Techio, V., Pereira, A.V., and Davide, L.C., Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids, Pesqui. Agropecu. Bras., 2017, vol. 52, no. 9, pp. 814817.

24. Souza-Sobrinho, F.D., Pereira, A.V., Ledo, F.J.D., Botrel, M.A., Oliveira, J.S.E., and Xavier, D.F., Agronomic evaluation of interespecific hybrids of elephant grass and pearl millet, Pesqui. Agropecu. Bras., 2005, vol. 40, pp. 873880.

25. Sundberg, E. and Glimelius, K., Effects of parental ploidy and genetic divergence on chromosome elimination and chloroplast segregation in somatic hydrids within Brassicaceae, Theor. Appl. Genet., 1991, vol. 83, pp. 8188.

26. Techio, V.H., Davide, L.C., and Pereira, A.V.P., Genomic analysis in Pennisetum purpureum and P. glaucum hybrids, Caryologia, 2005, vol. 8, pp. 2833.

27. Tiwari, V.K., Rawat, N., Neelam, K., Kumar, S., Randhawa, G.S., and Dhaliwal, H.S., Random chromosome elimination in synthetic TriticumAegilops amphiploids leads to development of a stable partial amphiploid with high grain micro-and macronutrient content and powdery mildew resistance, Genome, 2010, vol. 53, pp. 10531065.

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