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Effect of Cotreatment with static magnetic field and Cisdiamminedichloroplatinum (II) on apoptosis and cell cycle progression in HeLa cell line and Hu02
In this research, we evaluated the combined effect of static magnetic field and cisdiamminedichloroplatinum(II)(CDDP) on cell death, apoptosis and cell cycle in normal dermal fibroblast (Hu02) and HeLa cells. The cells were exposed to CDDP in the presence of 10 mT SMF for 24 and 48 hours. By using MTT assay the IC50 concentrations of CDDP were determined. The cytotoxic effects of this simultaneous application were studied using flowcytometric measurements for cell cycle and apoptosis. Based on the obtained results, the simultaneous application of CDDP and static magnetic field for 48 hours led to a significant increase in arrest of cell cycle progression of cells in G2/M phase, necrosis and late apoptosis in HeLa cells compared to the CDDPtreated group. In contrast, this combination led to a decrease in G2/M phase arrest, necrosis cells in Hu02 compared to drugtreated cells. In the presence of SMF, cancer cell susceptibility to CDDP was increased while it had some extent the protective effect on normal cells against cytotoxicity of the drug. Since there was an obvious difference in cell cycle stages and apoptosis between treated HeLa and Hu02 cells with combined SMF and CDDP, so this investigation proposes that cocompound of SMF and CDDP could be useful as way of reducing side effects of anticancer drug in normal cells and decreasing resistance of cancer cells to this drug.
Key words: apoptosis, cell cycle, CDDP, HeLa cell line, Hu02 cells, static magnetic field
E-mail: dr.hsoleimani arakmu.ac.ir
1. Basu, A. and Krishnamurthy, S., Cellular responses to cisplatin-induced DNA damage, J. Nucleic Acids, 2010, vol. 2010, p. 201367. https://doi.org/10.4061/2010/201367
2. Chen, W.F., Sun, R.G., Liu, Y., et al., Static magnetic fields enhanced the potency of cisplatin on K562 cells, Cancer Biother. Radiopharm., 2010, vol. 25, pp. 401–408. https://doi.org/10.1089/cbr.2009.0743
3. Chen, W.T., Lin, G.B., Lin, S.H., et al., Static magnetic field enhances the anticancer efficacy of capsaicin on HepG2 cells via capsaicin receptor TRPV1, PLoS One, 2018, vol. 13. e0191078.
4. de Gooijer, M.C., van den Top, A., Bockaj, I., et al., The G2 checkpoint—a node-based molecular switch, FEBS Open Bio, 2017, vol. 7, pp. 439–455. https://doi.org/10.1002/2211-5463.12206
5. Dini, L. and Abbro, L., Bioeffects of moderate-intensity static magnetic fields on cell cultures, Micron, 2005, vol. 36, pp. 195–217. https://doi.org/10.1016/j.micron.2004.12.009
6. Dunn, T.A., Schmoll, H.J., Grunwald, V., et al., Comparative cytotoxicity of oxaliplatin and cisplatin in non-seminomatous germ cell cancer cell lines, Invest. New Drugs, 1997, vol. 15, pp. 109–114.
7. El-Bialy, N.S. and Rageh, M.M., Extremely low-frequency magnetic field enhances the therapeutic efficacy of low-dose cisplatin in the treatment of Ehrlich carcinoma, BioMed. Res. Int., 2013, pp.189352–189358. https://doi.org/10.1155/2013/189352
8. Ghodbane, S., Lahbib, A., Sakly, M., et al., Bioeffects of static magnetic fields: oxidative stress, genotoxic effects, and cancer studies, BioMed. Res. Int., 2013, p. 602987. https://doi.org/10.1155/2013/602987
9. Hao, Q., Wenfang, C., Xia, A., et al., Effects of a moderate-intensity static magnetic field and adriamycin on K562 cells, Bioelectromagnetics, 2011, vol . 32, pp. 191–199. https://doi.org/10.1002/bem.20625
10. Jalali, A., Zafari, J., Javani Jouni, F., et al., Combination of static magnetic field and cisplatin in order to reduce drug resistance in cancer cell lines, Int. J. Radiat. Biol., 2019, vol. 95, pp. 1194–1201. https://doi.org/10.1080/09553002.2019.1589012
11. Ji, W., Huang, H., Deng, A., et al., Effects of static magnetic fields on Escherichia coli, Micron, 2009, vol. 40, pp. 894–898. https://doi.org/10.1016/j.micron.2009.05.010
12. Kamalipooya, S., Abdolmaleki, P., Salemi, Z., et al., Simultaneous application of cisplatin and static magnetic field enhances oxidative stress in HeLa cell line, In Vitro Cell Dev. Biol. Anim., 2017, vol. 53, pp. 783–790. https://doi.org/10.1007/s11626-017-0148-z
13. Kula, B., Sobczak, A., and Kuska, R., Effects of electromagnetic field on free- radical processes in steelworkers, part I: magnetic field influence on the antioxidant activity in red blood cells and plasma, J. Occup. Health, 2002, vol. 44, pp. 226–229.
14. Lengauer, C., Kinzler, K.W., and Vogelstein, B., Genetic instabilities in human cancers, Nature, 1998, vol. 396, pp. 643–649. https://doi.org/10.1038/25292
15. Liu, Y., Qi, H., Sun, R.G., et al., An investigation into the combined effect of static magnetic fields and different anticancer drugs on K562 cell membranes, Tumori, 2011, vol. 97, pp. 386–392. https://doi.org/10.1700/912.10039
16. Masuda, H., Tanaka, T., and Takahama, U., Cisplatin generates superoxide anion by interaction with DNA in a cell-free system, Biochem. Biophys. Res. Commun., 1994, vol. 203, pp. 1175–1180. https://doi.org/10.1006/bbrc.1994.2306
17. Miyakoshi, J., Effects of static magnetic fields at the cellular level, Prog. Biophys. Mol. Biol., 2005, vol. 87, pp. 213–223. https://doi.org/10.1016/j.pbiomolbio.2004.08.008
18. Sabo, J., Mirossay, L., Horovcak, L., et al., Effects of static magnetic field on human leukemic cell line HL-60, Bioelectrochemistry, 2002, vol. 56, pp. 227–231.
19. Sarvestani, A.S., Abdolmaleki, P., Mowla, S.J., et al., Static magnetic fields aggravate the effects of ionizing radiation on cell cycle progression in bone marrow stem cells, Micron, 2010, vol. 41, pp. 101–104. https://doi.org/10.1016/j.micron.2009.10.007
20. Satari, M., Javani Jouni, F., Abolmaleki, P., et al., Influence of static magnetic field on HeLa and Huo2 Cells in the presence of Aloe Vera extract, Asian Pac. J. Cancer Prev., 2020, vol. 21, pp. 9–15. https://doi.org/10.22034/apjcp.2020.21.s2.9
21. Sengupta, S. and Balla, V.K., A review on the use of magnetic fields and ultrasound for non-invasive cancer treatment, J. Adv. Res., 2018, vol. 14, pp. 97–111. https://doi.org/10.1016/j.jare.2018.06.003
22. Tenuzzo, B., Chionna, A., Panzarini, E., et al., Biological effects of 6 mT static magnetic fields: a comparative study in different cell types, Bioelectromiagnetics, 2006, vol. 27, pp. 560–577. https://doi.org/10.1002/bem.20252
23. Torgovnick, A. and Schumacher, B., DNA repair mechanisms in cancer development and therapy, Front. Genet., 2015, vol. 6, p. 157. https://doi.org/10.3389/fgene.2015.00157
24. Wagstaff, A.J., Brown, S.D., Holden, M.R., et al., Cisplatin drug delivery using gold- coated iron oxide nanoparticles for enhanced tumour targeting with external magnetic fields, Inorg. Chim. Acta, 2012, vol. 393, pp. 328–333. https://doi.org/10.1016/j.ica.2012.05.012
25. Wang, Q., Zheng, X.L., Yang, L., et al., Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells, J. Exp. Clin. Cancer Res., 2010, vol. 29, p. 159. https://doi.org/10.1186/1756-9966-29-159
26. Wozniak, K., Czechowska, A., and Blasiak, J., Cisplatin-evoked DNA fragmentation in normal, and cancer cells and its modulation by free radical scavengers and the tyrosine kinase inhibitor STI571, Chem. Biol. Interact., 2004, vol. 147, pp. 309–318. https://doi.org/10.1016/j.cbi.2004.03.001
27. Xu, L., Guo, W., Liu, Y., et al., Synergistic inhibitory effect of static magnetic field and antitumor drugs on Hepa1-6 cells, Sheng Wu Gong Cheng Xue Bao, 2015, vol. 31, pp. 1363–1374.
28. Zhang, Q.M., Tokiwa, M., Doi, T., et al., Strong static magnetic field and the induction of mutations through elevated production of reactive oxygen species in Escherichia coli soxR, Int. J. Radiat. Biol., 2003, vol. 79, pp. 281–286. https://doi.org/10.1080/0955300031000096289
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