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Current approaches to identification of Fusarium fungi infecting wheat

Karelov A.V., Borzykh O.I., Kozub N.O., Sozinov I.O., Yanse L.A., Sozinova O.I., Tkalenko H.M., Mishchenko L.T., Blume Ya.B.

Review 




SUMMARY. Fungi of the genus Fusarium are especially dangerous phytopathogens affecting common wheat (Triticum aestivum L.) among other crops, as they may cause not only crop losses but poisoning of humans and livestock. The review highlights current approaches to identify fungi of the genus Fusarium infecting common wheat. Microbiological techniques for identification of Fusa-rium species are still among laboratory protocols and recommendations, therefore some of the most popular genus- and species-specific media are mentioned in the review. However, in the modern literature, much more attention is paid to identification of Fusarium fungi with the use of the polymerase chain reaction (PCR). Therefore conventional PCR assays for identification of representatives of the genus Fusarium in general or only species producing especially dangerous metabolites (nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, 4-acetyl-nivalenol, enniatin) are highlighted in the review. The primer pairs to identify the presence of certain Fusarium species or their combinations in samples are described. For real-time PCR assays, which may be used for more precise quantitative and qualitative genus- and species-specific identification of Fusarium fungi, protocol details, primer and probe sequences are described, as well as recommended dyes are mentioned for the probes. For some primer pairs, additional details regarding their validation and the assay sensitivity are mentioned. Thus, the techniques described in the review are precise and comprehensive enough and may be used in combinations and separately for genus- and species-specific quantitative or qualitative identification of the fungi of the genus Fusarium.

Key words: Fusarium, wheat, Fusarium head blight, pink snow mold, molecular markers

Tsitologiya i Genetika 2021, vol. 55, no. 5, pp. 36-52

  1. Institute of Plant Protection of the National Academy of Agrarian Sciences of Ukraine, 03022, 33 Vasykivska St., Kyiv
  2. Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, 04123, 2a Osypovskogo St., Kyiv
  3. Education and Scientific Center Institute of Biology and Medicine of Taras Shevchenko National University of Kyiv, 01601, 64/13 Volodymyrska St., Kyiv

E-mail: hromogenblack ukr.net, natalkozub gmail.com, blume.yaroslav nas.gov.ua, lmishchenko ukr.net

Karelov A.V., Borzykh O.I., Kozub N.O., Sozinov I.O., Yanse L.A., Sozinova O.I., Tkalenko H.M., Mishchenko L.T., Blume Ya.B. Current approaches to identification of Fusarium fungi infecting wheat, Tsitol Genet., 2021, vol. 55, no. 5, pp. 36-52.

In "Cytology and Genetics":
A. V. Karelov, O. I. Borzykh, N. O. Kozub, I. O. Sozinov, L. A. Yanse, O. I. Sozinova, H. M. Tkalenko, L. T. Mishchenko & Ya. B. Blume Current Approaches to Identification of Fusarium Fungi Infecting Wheat, Cytol Genet., 2021, vol. 55, no. 5, pp. 433446
DOI: 10.3103/S0095452721050030


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