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he development of tumor-induced bystander effect and radiosensitivity of glioblastoma patients' peripheral blood lymphocytes with with different status of the MGMT gene in tumor cells

Zemskova O.V., Kurinnyi D.A., Rushkovsky S.R., Demchenko O.M., Romanenko M.G, Glavatsky O.Ya, Klymenko S.V.

 




SUMMARY. The effect of the MGMT gene methylation in glioblastoma cells on the development of the tumor-in-duced bystander effect and the modification of the sensitivity of untransformed bystander cells (peripheral blood lymphocytes) to the impact of ionizing radiation was studied. The results obtained demonstrate the development of the bystander effect, induced by the glioblastoma. There was no association found between the manifestation of the tumor-induced bystander effect in non-irradiated peripheral blood lymphocytes and the methylation status of the promoter region MGMT gene in the tumor cells. After the exposure to the radiation, statistically significant (p < 0,01) differences in apoptotic activity and radiosensitivity in peripheral blood lym-phocytes of glioblastoma patients with different status of the MGMT gene methylation in tumor cells were observed.

Key words: methylation MGMT, tumor-induced witness effect, Comet assay, glioblastoma

Tsitologiya i Genetika 2021, vol. 55, no. 2, pp. 24-31

  1. State Institution Institute of Neurosurgery. acad. A.P. Romodanova National Academy of Medical Sciences of Ukraine, street Platona Maiboroda, 32, Kyiv, 04050, Ukraine
  2. State Institution National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine, st. Yu. Ilyenko, 53, Kyiv, 04050, Ukraine
  3. Educational and Research Center Institute of Biology and Medicine of Taras Shevchenko National University of Kyiv, st. Volodymyrska, 64/13, Kyiv, 01601, Ukraine

E-mail: kurinnyi.d gmail.com

Zemskova O.V., Kurinnyi D.A., Rushkovsky S.R., Demchenko O.M., Romanenko M.G, Glavatsky O.Ya, Klymenko S.V. he development of tumor-induced bystander effect and radiosensitivity of glioblastoma patients' peripheral blood lymphocytes with with different status of the MGMT gene in tumor cells, Tsitol Genet., 2021, vol. 55, no. 2, pp. 24-31.

In "Cytology and Genetics":
O. V. Zemskova, D. A. Kurinnyi, S. R. Rushkovsky, O. M. Demchenko, M. G. Romanenko, O. Ya. Glavatsky & S. V. Klymenko Development of Tumor-Induced Bystander Effect and Radiosensitivity in the Peripheral Blood Lymphocytes of Glioblastoma Patients with Different MGMT Gene Methylation Statuses in Tumor Cells, Cytol Genet., 2021, vol. 55, no. 2, pp. 125131
DOI: 10.3103/S0095452721020158


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