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Construction of somatic hybrids among transgenic Solanum tuberosum and transplastomic Solanum rickii

Matveeva N.A., Shakhovsky A.M., Kuchuk N.V.

 


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The hybrid plants with transformed plastids were regenerated after fusion of mesophyll protoplasts of Solanum rickii with aadA chloroplast selective marker gene and Solanum tuberosum with nptII nuclear selective marker gene. The hybrid callus clones were selected on the medium containing streptomycine, spectinÓmycine and kanamycine. The hybrids were identified on the base of PCR analysis of nuclear and plastid DNAs. The analysis have shown that regenerated plants were somatic hybrids containing aadA and nptII genes, Solanum rickii and Solanum tuberosum nuclear and chloroplast DNA.

Tsitologiya i Genetika 2008, vol. 42, no. 4, pp. 38-44

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Matveeva N.A., Shakhovsky A.M., Kuchuk N.V. Construction of somatic hybrids among transgenic Solanum tuberosum and transplastomic Solanum rickii, Tsitol Genet., 2008, vol. 42, no. 4, pp. 38-44.




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