TSitologiya i Genetika 2008, vol. 42, no. 2, 16-20
Cytology and Genetics , vol. , no. , , doi: https://www.doi.org/

Purification of recombinant GFP produced by Agrobacterum-mediated transient expression in Nicotiana excelsior

Sindarovska Y.R., Sheludko Y.V., Gerasymenko I.M., Bannikova M.A., Kuchuk N.V.

Institute of cell biology and genetic engineering, Zabolotnogo str. 148, Kyiv 03680, Ukraine

Green fluorescent protein (GFP) is commonly used as a reporter protein in a wide range of biological experiments. The efficient protocol of Agrobacterium-mediated transient expression in Nicotiana excelsior was applied for quick preparative production of recombinant GFP. The protein purification scheme has been developed and included ammonium sulfate precipitation and Q-sepharose anion-exchange chromatography. It results in obtaining of a fraction with about 85 % GFP homogeneity and the protein yield of about 75 %.

Keywords: Nicotiana excelsior; Agrobacterium tumefaciens; transient expression; recombinant protein; GFP

TSitologiya i Genetika
2008, vol. 42, no. 2, 16-20

Current Issue
Cytology and Genetics
, vol. , no. , ,
doi:

Full text and supplemented materials

Free full text: PDF  

References