Accipiter nisus is a small to mediumsized raptor widely distributed across Eurasia and parts of Africa. In this study, we sequenced the complete mitochondrial genome of A. nisus from Mianyang using the Illumina platform. We then compared mitochondrial genome A. nisus variations across four regions and analyzed the phylogenetic relationship of this species within the genus Accipiter. The results indicate that the mitochondrial genome of A. nisus from Mianyang is 18,297 bp in length, which is smaller than those from Qufu (18,647 bp), Yeongyanggun (18,352 bp), and BukGu (19,417 bp). The genomes of all four individuals contain 37 genes, an A+Trich control region, and a pseudocontrol region, with the most notable length variation occurring in the pseudocontrol region. Additionally, inconsistencies in the usage of start and stop codons were observed in some proteincoding genes (PCGs). Phylogenetic analysis based on 13 PCGs and CYTB genes revealed that A. nisus is phylogenetically related to A. gentilis. This study provides essential genetic data and scientific evidence for A. nisus conservation.
Keywords: Accipiter nisus; complete mitochondrial genome; Comparative analysis; Phylogenetic relationship