SUMMARY. Wound management remains a significant clinical challenge, necessitating the development of advanced biomaterial solutions. This study aimed to evaluate the wound healing potential of novel chitosan-hya-luronic acid-based hydrogel supplemented with N-stea-roylethanolamine (NSE). Two hydrogel systems were developed: one with and one without the anti-inflam-matory cannabimimetic NSE. Morphology was studied using scanning electron microscopy, biocompatibility and biodegradability were tested using murine fibro-blasts by MTT assay and neutral red staining. Wound healing was evaluated in C57 black laboratory mice using histological analysis with hematoxylin staining. The hydrogels demonstrated high biocompatibility, enhanced fibroblast migration, and induced lysosomal activity, indicating biodegradability. Both hydrogel sys-tems accelerated wound healing in mice, with faster epithelialization and remodeling of wound tissues ob-served in histological sections. Acceleration of wound healing was found at the use of both types of the created gel films – with and without the NSE, that was confirmed by faster epithelialization and remodeling of wound tissues observed in the histological sections.
Keywords: Chitosan, hydrogels, N-stearoyl ethanolamine, wound dressing, wound healing
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