TSitologiya i Genetika 2024, vol. 58, no. 5, 3-17
Cytology and Genetics 2024, vol. 58, no. 5, 371–384, doi: https://www.doi.org/10.3103/S0095452724050050

Identification of FtsZ interdomain cleft effectors based on pharmacophore search and molecular docking

P.A. Karpov, D.S. Ozheriedov, S.P. Ozheredov, O.M. Demchuk, Ya.B. Blume

  • Institute of Food Biotechnology and Genomics NAS of Ukraine, Baidy-Vyshnevetskoho str., 2A, Kyiv, 04123, Ukraine

SUMMARY. There are a significant number of inhibitors of the bacterial FtsZ protein, the biological activity of which has been confirmed biochemically, but their binding sites remain unclear. This significantly complicates further combinatorial design, and in the current study we present the results of a computational search for effectors of the Inter-Domain Cleft (IDC) site. The actual research was based on the results of pharmacophore screening using the Pharmit service and molecular docking with CCDC GOLD and iGEMDOCK programs. The objective group was a combined library of 379 compounds, which was designed based on revision of the structural database of the RCSB Protein Data Bank and compounds from the ChEMBL database, for which direct interaction with FtsZ has been proven biochemically. According to the results of pharmacophore search, docking and structural analysis, 88 effectors of the IDC site were identified. One more curcumin compound has been identified as a potential IDC site effector.

Keywords: FtsZ, IDC, effectors, ligand-protein interaction, pharmacophore search, molecular docking

TSitologiya i Genetika
2024, vol. 58, no. 5, 3-17

Current Issue
Cytology and Genetics
2024, vol. 58, no. 5, 371–384,
doi: 10.3103/S0095452724050050

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References

Adams, D.W., Wu, L.J., Czaplewski, L.G., et al., Multiple effects of benzamide antibiotics on FtsZ function, Mol. Microbiol., 2011, vol. 80, pp. 68–84. https://doi.org/10.1111/j.1365-2958.2011.07559.x

Alnami, A., Norton, R.S., Pena, H.P., et al., Conformational flexibility of a highly conserved helix controls cryptic pocket formation in FtsZ, J. Mol. Biol., 2021, vol. 433, no. 15, p. 167061. https://doi.org/10.1016/j.jmb.2021.167061

Andreu, J.M., Schaffner-Barbero, C., and Huecas, S., The antibacterial cell division inhibitor PC190723 is an FtsZ polymer-stabilizing agent that induces filament assembly and condensation, J. Biol. Chem., 2010, vol. 285, no. 19, pp. 14239–14246. https://doi.org/10.1074/jbc.M109.094722

Andreu, J.M., Huecas, S., Araujo-Bazan, L., et al., The search for antibacterial inhibitors targeting cell division protein FtsZ at its nucleotide and allosteric binding sites, Biomedicines, 2022, vol. 10, no. 8, p. 1825. https://doi.org/10.3390/biomedicines10081825

Bellaver, E.H., da Costa, I.M., Redin, E.E., et al., The fermented milk can be a natural ally against obesity? Investigation of bovine milk fermentation by Lacticaseibacillus casei LBC 237, screening, and in silico predictions of bioactive peptides for obesity control, Intelligent Pharmacy (in press). https://doi.org/10.1016/j.ipha.2024.05.009

Bryan, E., Ferrer-Gonzalez, E., Sagong, H.Y., et al., Structural and antibacterial characterization of a new benzamide FtsZ inhibitor with superior bactericidal activity and in vivo efficacy against multidrug-resistant Staphylococcus aureus, ACS Chem. Biol., 2023, vol. 18, no. 3, pp. 629–642. https://doi.org/10.1021/acschembio.2c00934

Cramer, P., AlphaFold2 and the future of structural biology, Nat. Struct. Mol. Biol., 2021, vol. 28, no. 9, pp. 704–705. https://doi.org/10.1038/s41594-021-00650-1

Ferrer-González, E., Fujita, J., Yoshizawa, T., et al., Structure-guided design of a fluorescent probe for the visualization of FtsZ in clinically important gram-positive and gram-negative bacterial pathogens, Sci. Rep., 2019, vol. 9, no. 1, p. 20092. https://doi.org/10.1038/s41598-019-56557-x

Fujita, J., Harada, R., Maeda, Y., et al., Identification of the key interactions in structural transition pathway of FtsZ from Staphylococcus aureus, J. Struct. Biol., 2017a, vol. 198, no. 2, pp. 65–73. https://doi.org/10.1016/j.jsb.2017.04.008

Fujita, J., Maeda, Y., Mizohata, E., et al., Structural flexibility of an inhibitor overcomes drug resistance mutations in Staphylococcus aureus FtsZ, ACS Chem. Biol., 2017b, vol. 12, no. 7, pp. 1947–1955. https://doi.org/10.1021/acschembio.7b00323

Gurnani, M., Rath, P., Chauhan, A., et al., Inhibition of Filamentous thermosensitive mutant-Z protein in Bacillus subtilis by cyanobacterial bioactive compounds, Molecules, 2022, vol. 27, no. 6, p. 1907. https://doi.org/10.3390/molecules27061907

Han, H., Wang, Z., Li, T., et al., Recent progress of bacterial FtsZ inhibitors with a focus on peptides, FEBS J., 2021, vol. 288, no. 4, pp. 1091–1106. https://doi.org/10.1111/febs.15489

Haydon, D.J., Stokes, N.R., Ure, R., et al., An inhibitor of FtsZ with potent and selective anti-staphylococcal activity, Science, 2008, vol. 321, no. 5896, pp. 1673–1675. https://doi.org/10.1126/science.1159961

Hendlich, M., Rippmann, F., and Barnickel, G., LIGSITE: automatic and efficient detection of potential small molecule-binding sites in proteins, J. Mol. Graph. Model, 1997, vol. 15, no. 6, pp. 359–363. https://doi.org/10.1016/s1093-3263(98)00002-3

Hirano, Y., Okimoto, N., Fujita, S., et al., Molecular dynamics study of conformational changes of Tankyrase 2 binding subsites upon ligand binding, ACS Omega, 2021, vol. 6, no. 27, pp. 17609–17620. https://doi.org/10.1021/acsomega.1c02159

Huecas, S., Araújo-Bazán, L., Ruiz, F.M., et al., Targeting the FtsZ allosteric binding site with a novel fluorescence polarization screen, cytological and structural approaches for antibacterial discovery, J. Med. Chem., 2021, vol. 64, no. 9, pp. 5730–5745. https://doi.org/10.1021/acs.jmedchem.0c02207

Jones, G., Willett, P., Glen, R.C., et al., Development and validation of a genetic algorithm for flexible docking, J. Mol. Biol., 1997, vol. 267, no. 3, pp. 727–748. https://doi.org/10.1006/jmbi.1996.0897

Jumper, J., Evans, R., Pritzel, A., et al., Highly accurate protein structure prediction with AlphaFold, Nature, 2021, vol. 596, no. 7873, pp. 583–589. https://doi.org/10.1038/s41586-021-03819-2

Karpov, P.A., Demchuk, O.M., Britsun, V.M., et al., New imidazole inhibitors of Mycobacterial FtsZ: the way from high-throughput molecular screening in Grid up to in vitro verification, Nauka Innovats., 2016, vol. 12, no. 3, pp. 44–59. https://doi.org/10.15407/scin12.03.044

Kifayat, S., Yele, V., Ashames, A., et al., Filamentous temperature sensitive mutant Z: a putative target to combat antibacterial resistance, RSC Adv., 2023, vol. 13, no. 17, pp. 11368–11384. https://doi.org/10.1039/d3ra00013c

Löwe, J. and Amos, L.A., Crystal structure of the bacterial cell-division protein FtsZ, Nature, 1998, vol. 391, no. 6663, pp. 203–206. https://doi.org/10.1038/34472

Matsui, T., Yamane, J., Mogi, N., et al., Structural reorganization of the bacterial cell-division protein FtsZ from Staphylococcus aureus, Acta Crystallogr., Sect. D: Biol. Crystallogr., 2012, vol. 68, pp. 1175–1188. https://doi.org/10.1107/S0907444912022640

Ozheriedov, D.S., Ozheredov, S.P., Demchuk, O.M., Blume, Ya.B., and Karpov, P.A., Ligand-induced variability of the FtsZ protein InterDomain Cleft site pocket, Cyt. Genet., 2024, vol. 58, no. 4. https://doi.org/IN PRINT

Pradhan, P., Margolin, W., and Beuria, T.K., Targeting the achilles heel of FtsZ: the interdomain cleft, Front. Microbiol., 2021, vol. 12, p. 732796. https://doi.org/10.3389/fmicb.2021.732796

Ramírez-Aportela, E., López-Blanco, J.R., Andreu, J.M., et al., Understanding nucleotide-regulated FtsZ filament dynamics and the monomer assembly switch with large-scale atomistic simulations, Biophys. J., 2014, vol. 107, no. 9, pp. 2164–2176. https://doi.org/10.1016/j.bpj.2014.09.033

Sharma, A.K., Poddar, S.M., Chakraborty, J., et al., A mechanism of salt bridge-mediated resistance to FtsZ inhibitor PC190723 revealed by a cell-based screen, Mol. Biol. Cell., 2023, vol. 34, no. 3, p. ar16. https://doi.org/10.1091/mbc.E22-12-0538

Steinkellner, G., Rader, R., and Thallinger, G.G., VASCo: computation and visualization of annotated protein surface contacts, BMC Bioinf., 2009, vol. 10, p. 32. https://doi.org/10.1186/1471-2105-10-32

Stokes, N.R., Baker, N., Bennett, J.M., et al., An improved small-molecule inhibitor of FtsZ with superior in vitro potency, drug-like properties, and in vivo efficacy, Antimicrob. Agents Chemother., 2013, vol. 57, no. 1, pp. 317–325. https://doi.org/10.1128/AAC.01580-12

Sunseri, J., Koes, D.R., Pharmit: interactive exploration of chemical space, Nucleic Acids Res., 2016, vol. 44, no. W1, pp. W442–W448. https://doi.org/10.1093/nar/gkw287

Tan, C.M., Therien, A.G., Lu, J., Lee, S.H., et al., Restoring methicillin-resistant Staphylococcus aureus susceptibility to β-lactam antibiotics, Sci. Transl. Med., 2012, vol. 4, no. 126, p. 126ra35. https://doi.org/10.1126/scitranslmed.3003592

UniProt Consortium, UniProt: the Universal Protein Knowledgebase in 2023, Nucleic Acids Res., 2023, vol. 51, no. D1, pp. D523–D531. https://doi.org/10.1093/nar/gkac1052

Wagstaff, J.M., Tsim, M., Oliva, M.A., et al., Polymerization-associated structural switch in FtsZ that enables treadmilling of model filaments, mBio, 2017, vol. 8, no. 3, p. e00254-17. https://doi.org/10.1128/mBio.00254-17

Wang, M., Fang, C., Ma, B., et al., Regulation of cytokinesis: FtsZ and its accessory proteins, Curr. Genet., 2020, vol. 66, no. 1, pp. 43–49. https://doi.org/10.1007/s00294-019-01005-6

Wang, M., Wu, Z., Wang, J., et al., Genetic algorithm-based receptor ligand: A genetic algorithm-guided generative model to boost the novelty and drug-likeness of molecules in a sampling chemical space, J. Chem. Inf. Model., 2024, vol. 64, no. 4, pp. 1213–1228. https://doi.org/10.1021/acs.jcim.3c01964

Waterhouse, A., Bertoni, M., Bienert, S., et al., SWISS-MODEL: homology modelling of protein structures and complexes, Nucleic Acids Res., 2018, vol. 46, no. W1, pp. W296–W303. https://doi.org/10.1093/nar/gky427

Yang, J.M., Development and evaluation of a generic evolutionary method for protein-ligand docking, J. Comput. Chem., 2004, vol. 25, no. 6, pp. 843–857. https://doi.org/10.1002/jcc.20013

Yang, J.M. and Chen, C.C., GEMDOCK: a generic evolutionary method for molecular docking, Proteins, 2004, vol. 55, no. 2, pp. 288–304. https://doi.org/10.1002/prot.20035