SUMMARY. Garden asparagus contains unique compo-unds that can stop the development of cancerous tumors and even destroy cancer cells. However, there is still insufficient information about the features of the biosynthesis of these compounds and the conditions for their production. In this paper, the influence of an inorganic nitrogen source on the growth of a suspended culture of garden asparagus, the accumulation of phenolic compounds in the culture and culture liquid, as well as the synthesis of saponins is studied. A callus and suspension culture of garden asparagus Asparagus officinalis L. was obtained under in vitro conditions. For further studies, the concentrations of growth regulators 1 mg/l of kinetin and 1.5 mg/l of 2.4-D were selected. The fastest growth of the suspension culture was established on a medium with a concentration of potassium nitrate 1,900 mg/l and ammonium sulfate 250 mg/l. Individual explants showed spontaneous differentiation of callus tissue, which affected the increase in the mass of callus and the ratio of the concentration of exo-phenols to the total mass of the explant. The highest concentrations of endo- and exo-phenols were obtained on a medium without ammonium nitrogen sources. A positive effect of a medium with an ammonium sulfate concentration of 500 mg/l on the synthesis of the main steroid saponins of Asparagus officinalis L. was determined. As a result of the study, a modified MS medium was developed that promotes the accumulation of steroid saponins in a suspension culture of garden asparagus.
Keywords: medicinal asparagus, suspension culture, biologically active substances, phenols, steroid saponins, nitrogen source
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