Aeluropus littoralis is a valuable halophyte grass belonging to the same family of wheat and is used as forage. Although A. littoralis has the potential to become an important genetic resource for improving salt and drought tolerance in economically important crops, no SSR markers have been developed for it. The main goal was to rapidly develop a set of genic SSR markers for A. littoralis. Repeat analysis of non-redundant EST sequences of Aeluropus and transferability assessment of 110 SSR-rich loci from rice and wheat were used to identify EST-SSRs. Then selected EST-SSR loci and some physiological traits includings Na+, K+ and Ash content were utilized for marker characterization and assessment of genetic diversity among A. littoralis accessions collected from all around the country. The results showed that 6.7 % of EST records of A. littoralis comprising SSR motifs which was used for desiging 18 primer pairs (ALES). In addition 48 SSR loci (GDES) from 110 of the gramineae were shown to be transferable to A. littoralis based on the PCR profiles. Finally, genotypic clustering based on EST-SSR markers divided the accessions into seven groups. The accessions were also categorized into six groups according to the physiological traits. Our finding indicated that there are remarkable variations about 33 % in coding regions of Iranian Aeluropus accessions. The results of both genotypic and physiologic clustering were partially consistent and most groups corresponded to geographic regions.
Keywords: EST-SSRs, EST Repeat analysis, Genetic variation, Transferability
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References
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