Phospholipase Cγ2 has a promotive effect on hepatic carcinoma development. Meanwhile, lncRNAs play a critical role in the pathology. Therefore, to determine whether phospholipase Cγ2 could enhance HCC cell proliferation by regulating lncRNA transcription, hepatic carcinoma cells RH35 were infected with Adphospholipase Cγ2 constructed previously, followed by lncRNA sequencing by highthroughput technology. Differently expressed lncRNAs (DElncRNAs) and their target genes were identified according to strict criteria. GO and KEGG, Reactome pathway analyses were performed to analyze biological processes and the related pathways of DElncRNAs. lncRNA/mRNA coexpression pairs were screened according to expression profiling combined with bioinformatics analysis. The results showed that 231 DElncRNAs were identified in Adphospholipase Cγ2overexpressing cells compared to control, containing 60 up and 171 downregulated ones. Target genes prediction analysis showed that 61 cis and 30 transacting DElncRNAs were matched to 55 and 26 targets, respectively. Coexpression analysis found 33 lncRNA/mRNA coexpression pairs including 24 pairs in cis. GO analysis showed that these cismode lncRNA/mRNA pairs were involved in cytoskeleton organization, cell adhesion, and multiple signaling pathways related to apoptosis, proliferation, and metastasis. Collectively, phospholipase Cγ2 caused significant alterations in the expression of many lncRNAs in liver cancer cells, providing valuable insight into the precise mechanism of phospholipase Cγ2promoting liver cancer cell growth.
Keywords: phospholipase Cγ2; hepatic carcinoma; lncRNA transcriptome; bioinformatic analysis
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