TSitologiya i Genetika 2023, vol. 57, no. 3, 40-47
Cytology and Genetics 2023, vol. 57, no. 3, 239–245, doi: https://www.doi.org/10.3103/S009545272303009X

The effect of adaptive stress factors on the functioning of Clostridium sp. cells – producers of biobutanol

Tigunova O.O., Bratishko V.V., Shulga S.M.

  1. SE «Institute of Food Biotechnology and Genomics
    of the National Academy of Sciences of Ukraine», 2 a, Baidy Vyshnevetskoho str., Kyiv, Ukraine, 04123
  2. National University of Life and Enveronmental science of Ukraine, 15, Heroiv Oborony str., Kiev, Ukraine, 03041

SUMMARY. Biobutanol is one of the types of biofuel obtained by microbial synthesis (acetone-butanol-ethanol fermentation). Using adaptive cell changes, it is possible to increase the level of accumulation of the target product due to the effect of stress factors. For this aim, the effect of the following stress factors: allopurinol, furfural, zinc, oxygen and glycerol as a non-specific substrate on the functioning of Clostridium sp cells was investigated. Stress factors were added to the medium in adapting doses. It was found that the concentration of butanol was the greatest (1.2 g/l) when 0.5 g/l of furfural was added to the medium compared to the control (0.6 g/l). It was shown that the use of adaptive dose (0.001 g/l) of ZnSO4 resulted in the greatest concentration of butanol (1.0 g/l), and the highest concentration of butanol (1.3 g/l) was obtained when using glucose and glycerol in the ratio of 1 : 3 as a carbon source. It was demonstrated that the concentration of butanol in the culture medium increa-sed (0.8 g/l) at a concentration of allopurinol in the medium of 0.025 g/l. The possibility of using adaptive stress to increase the accumulation of the target product due to changes in metabolic pathways in the cells of microorganisms, indirectly due to the action of stress factors, is substantiated.

Keywords: Clostridium, producer strain, butanol, stress factors, allopurinol, furfural, zinc, oxygen, glycerol

TSitologiya i Genetika
2023, vol. 57, no. 3, 40-47

Current Issue
Cytology and Genetics
2023, vol. 57, no. 3, 239–245,
doi: 10.3103/S009545272303009X

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