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Influence of high-carbohydrate and high-lipid diet on the enzymatic link of antioxidant protection and the level of oxidatively modified proteins and lipids in rat erythrocytes

Nagalievska M.R., Petryn T.S., Sybirna N.O.

 




SUMMARY. The to influence of high-lipid and high-carbohydrate diet, as inducers of the development of metabolic syndrome on the prooxidant-antioxidant state of rat erythrocytes have been studied. Oxidative damage of cellular components was evaluated by the content of thiobarbituric acid reactive substances (TBARS), as well as products of oxidative modification of proteins of neutral and basic nature. The antioxidant potential of erythrocytes was determined by the activity of superoxide dismutase, catalase and glutathione peroxi-dase. Manipulations with diets, especially high-lipid diet in which an additional source of lipids was olive oil and high-carbohydrate diet, which involved the consumption of animals 10 % fructose solution, promoting the development of oxidative stress in rats’ erythrocytes. It has been shown that a 28-day high-lipid diet does not support significant variables in TBARS and oxidized protein modification products, but demonstrates sig-nificant reduced catalase and glutathione peroxidase activity in hemolytic erythrocytes. The longer duration of exposure to the high-lipid diet (42 days) was ac-companied by an increase in the content of TBARS against the background of reduced superoxide dismutase and catalase activity. When using fructose for 28 days for the induction of metabolic syndrome, no significant changes in the content of products of oxidative modification of lipids and protein were shown. Instead, a longer effect of a high-carbohydrate diet (42 days) is accompanied by an increase in the content of TBARS. The use of fructose for 28 days causes a decrease in the activity of superoxide dismutase, catalase and glu-tathione peroxidase. A similar tendency to decrease the activity of the studied enzymes was demonstrated when animals consumed fructose for 42 days. The obtained results allow us to conclude that the assessment of redox balance of erythrocytes in metabolic syndrome is a rational and convenient method of studying the prooxidant-antioxidant state of the body.

Tsitologiya i Genetika 2022, vol. 56, no. 1, pp. 3-12

  • Ivan Franko National University of Lviv, Hrushevskyi St., 4, Lviv, 79005, Ukraine

E-mail: mariia.nagalievska lnu.edu.u

Nagalievska M.R., Petryn T.S., Sybirna N.O. Influence of high-carbohydrate and high-lipid diet on the enzymatic link of antioxidant protection and the level of oxidatively modified proteins and lipids in rat erythrocytes, Tsitol Genet., 2022, vol. 56, no. 1, pp. 3-12.




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