TSitologiya i Genetika 2022, vol. 56, no. 6, 83-84
Cytology and Genetics 2022, vol. 56, no. 6, 567–573, doi: https://www.doi.org/10.3103/S0095452722060081

The influence of GFRα1 inhibition on proliferation and apoptosis of spermatogenic cells

Mao J., Liu P., Han W., Mo R., Guo S., Sun J.

  1. Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Department of Andrology, Shanghai 200137
  2. Shanghai University of Traditional Chinese Medicine, Graduate School Shanghai 200137, China

The role of the GFRα1 receptor in the regulation of cell differentiation and spermatogenesis is poorly investigated. The influence of GFRα1 on the apoptosis and proliferation of spermatogenic cells are not fully elucidated. In this study, GC­1 spg cells were cultured and then assigned to a control cell (control group) transfected with empty adenovirus AdH1­siRNA and an inhibited cells (inhibited group) with GFRα1 interfering adenovirus (AdH1­siRNA/GFRα1). The GFRα1 inhibition was determined by employing western blot and qRT­PCR analysis. Furthermore, we used CCK8 for determining the proliferation of the GFRα1 interfering adenovirus (AdH1­siRNA/GFRα1). Transfected cells, employing flow cytometry and Caspase 3, Bax and Bcl­2 expression analysis to determine their apoptosis. This study demonstrated that after transfection, the inhibited cells presented lower proliferation and invasion and higher apoptosis. According to these findings, the inhibition of GFRα1 might reduce spermatogenesis and remarkably affect spermatogenic cells proliferation.

Keywords: GFRα1; spermatogenic cell; apoptosis; GC­1 spg cells, cells proliferation

TSitologiya i Genetika
2022, vol. 56, no. 6, 83-84

Current Issue
Cytology and Genetics
2022, vol. 56, no. 6, 567–573,
doi: 10.3103/S0095452722060081

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