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The influence of GFRα1 inhibition on proliferation and apoptosis of spermatogenic cells
The role of the GFRα1 receptor in the regulation of cell differentiation and spermatogenesis is poorly investigated. The influence of GFRα1 on the apoptosis and proliferation of spermatogenic cells are not fully elucidated. In this study, GC1 spg cells were cultured and then assigned to a control cell (control group) transfected with empty adenovirus AdH1siRNA and an inhibited cells (inhibited group) with GFRα1 interfering adenovirus (AdH1siRNA/GFRα1). The GFRα1 inhibition was determined by employing western blot and qRTPCR analysis. Furthermore, we used CCK8 for determining the proliferation of the GFRα1 interfering adenovirus (AdH1siRNA/GFRα1). Transfected cells, employing flow cytometry and Caspase 3, Bax and Bcl2 expression analysis to determine their apoptosis. This study demonstrated that after transfection, the inhibited cells presented lower proliferation and invasion and higher apoptosis. According to these findings, the inhibition of GFRα1 might reduce spermatogenesis and remarkably affect spermatogenic cells proliferation.
Key words: GFRα1; spermatogenic cell; apoptosis; GC1 spg cells, cells proliferation
E-mail: shun31316jit 163.com
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