ISSN 0564-3783  



Main page
Contacts
Themes
Archive  
Themes
Subscription
Information to authors
Editorial board
Mobile version


In Ukrainian

Export citations
UNIMARC
BibTeX
RIS





Humic acid has protective effect on gastric ulcer by alleviating inflammation in rats

Şehitoğlu M.H., Öztopuz Ö., Karaboğa İ., Ovali M.A., Uzun M.

 




The new agents are needed in treatment of gastric ulcer that have less side effects, adequate efficacy, and no drug interactions. In this study, we aimed to investigate the potential protective effects of humic acid on experimental gastric ulcer. Wistar Albino male rats (n = 48) were randomly divided into 8 groups as follow; Control (without any applications), Humic acid (50 mg/kg), ethanol group (1 ml/rat), and indomethacin group (25 mg/kg). In the treatment groups, both gastric ulcer model and humic acid 50 mg/kg were applied. In addition, famotidine the antiulcer drug was used as positive control. All medications were administered by oral gavage. Levels of ADAM10 and ADAMTS12 in gastric mucosa were determined by ELISA method. HematoxylinEosin (H&E) staining, iNOS, and PCNA immunohistochemical staining were performed for histopathological investigations. Apoptosis was demonstrated by using the TUNEL method. In addition, the levels of inflammatory cytokines (TNFα, IL6, IL10) and caspase3 gene were determined by qRTPCR. ADAM10 and ADAMTS12 levels significantly increased in the treatment groups compared to the ulcer groups (p < 0.05). The experimental groups showed mucosal erosion, bleeding, leukocyte infiltration and edema. Treatment with humic acid and famotidine was found to suppress iNOS activity, thereby decreasing proinflammatory activity and preventing damage to the gastric mucosa, while reducing the number of apoptotic cells. IL6, IL10, TNFα and caspase3 levels were significantly decreased in the treatment groups compared to damaged gastric mucosa. As a result, humic acid may be defined as a potential protective agent with its antiinflammatory effect in gastric ulcer.

Key words: Gastric mucosa injury, Humic acid, ADAM10, ADAMTS12, Inflammation, Famotidine

Tsitologiya i Genetika 2022, vol. 56, no. 1, pp. 79-82

  1. Department of Medical Biochemistry, Faculty of Medicine, Çanakkale Onsekiz Mart University, 17100, Çanakkale, Turkey
  2. Department of Biophysics, Faculty of Medicine, Çanakkale Onsekiz Mart University, 17100, Çanakkale, Turkey
  3. Department of Emergency and Disaster Management, School of Health, Edirne Namık Kemal University, 59030, Tekirdağ, Turkey
  4. Department of Physiology, Faculty of Medicine, Çanakkale Onsekiz Mart University, 17100, Çanakkale, Turkey

E-mail: hill_al hotmail.com, hilals comu.edu.tr

Şehitoğlu M.H., Öztopuz Ö., Karaboğa İ., Ovali M.A., Uzun M. Humic acid has protective effect on gastric ulcer by alleviating inflammation in rats, Tsitol Genet., 2022, vol. 56, no. 1, pp. 79-82.




Copyright© ICBGE 2002-2023 Coded & Designed by Volodymyr Duplij Modified 01.02.23