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4-Hydroxycoumarin effects on both cellular and genetic characteristics of hepatocellular carcinoma cells

Öner Ç., Soyergin D., Özyurt A., Çolak E.

 




4-Hydroxycoumarin is an aromatic substance which is metabolized in liver and used as a therapeutic agent for various diseases. We aimed to determine the impact of 4-Hydroxycoumarin on HepG2 cells according to their viability, proliferation, adhesion and gene expression of cellular behavior parameters. Inhibitory concentration 50 (IC50) of 4-Hydroxycoumarin was detected on HepG2 cells. After determining the optimal time and concentration, the effect of 4-Hydroxycoumarin on viability, proliferation and adhesion of HepG2 cells were observed. Gene expressions of Ki-67, MMP-2, MMP-9 and piR-823 expression were determined by using Real Time-Polymerase Chain Reaction. IC50 value of 4-Hydroxycoumarin on HepG2 cells was 5 M at the 48th hour (p < 0.001). 5 M at the 48th hour of 4-Hydroxycoumarin caused to decrease of proliferation (p < 0.001) and viability of HepG2 cells (p < 0.001). Viability rate were supported by hematoxylin-eosin staining. Adhesion of cells increased on 4-Hydroxycoumarin treated cells compared to control (p < 0.001). While Ki-67 gene expression of 4-Hydroxycoumarin treated group decreased (p < 0.001); upregulation of MMP-2, MMP-9 and piR-823 expressions were determined in 4-Hydroxycoumarin treated group (p < 0.001). According to the cellular and genetic perspective, 4-Hydroxycoumarin might be useful to treat hepatocellular carcinoma. High adhesion and proliferation are the main characteristics of HepG2 cells, 4-Hydroxycoumarin treatment caused to lose these functions. The genetic markers of these characteristics also supported the same result. These are first findings about the effect of 4-Hydroxycoumarin on piR-823 and genes which are key features of cellular survival mechanisms.

Key words: 4-Hydroxycoumarin, Matrix metalloproteinase, piRNA, piR-823, Hepatocellular carcinoma

Tsitologiya i Genetika 2022, vol. 56, no. 3, pp. 76-79

  1. Maltepe University, Medical Faculty, Department of Medical Biology and Genetics, İstanbul, Turkey
  2. Maltepe University, Medical Faculty, İstanbul, Turkey
  3. Eskişehir Osmangazi University, Medical Faculty, Department of Biostatistics, Eskişehir, Turkey

E-mail: cagri.oner maltepe.edu.tr

Öner Ç., Soyergin D., Özyurt A., Çolak E. 4-Hydroxycoumarin effects on both cellular and genetic characteristics of hepatocellular carcinoma cells, Tsitol Genet., 2022, vol. 56, no. 3, pp. 76-79.

In "Cytology and Genetics":
Çağri Öner, Dilara Soyergin, Ahmet Özyurt & Ertuğrul Çolak 4-Hydroxycoumarin Effects on Both Cellular and Genetic Characteristics of Hepatocellular Carcinoma Cells, Cytol Genet., 2022, vol. 56, no. 3, pp. 292300
DOI: 10.3103/S0095452722030094


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