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Characterization and phylogeny analysis of Azerbaijan tea (Camellia sinensis L.) genotypes by molecular markers

Huseynov M., Suleymanova Z., Ojaghi J., Mammadov A.

 




The tea is one of the most important products in the southern region of Azerbaijan Republic and plays an essential role in the regions economy. Assessing and describing genetic diversity in crop plants is a crucial first step toward their improvement. In this study the tea genotypes from the southern region of Azerbaijan were studied using 10 random amplified polymorphic DNA in order to estimate their genetic diversity and to identify the relationships among their genotypes. The RAPD primers generated 132 amplification products, 113 of which were polymorphic. The polymorphic banding patterns with the number of amplified fragments varied from 4 (OPA-19) to 19 (OPAB-18). Percent polymorphism ranged from 50 to 95 % with an average of 83.94 %. The genetic similarity among the genotypes tested ranged 0.445 to 0.819 with an average of 0.512. The cluster analysis based on UPGMA and Jaccard similarity index revealed 5 main clusters for the RAPD data and the principal coordinate analysis (PCoA) supported the clustering result. According to our results, there is a relatively high genetic distance across tea genotypes in the south of Azerbaijan Republic. Furthermore, it could be inferred that RAPD markers are suitable tools for the evaluation of genetic diversity and relationships within Camellia sinensis.

Key words: Camellia sinensis, RAPD marker, genetic variation, PCoA, polymorphism

Tsitologiya i Genetika 2022, vol. 56, no. 3, pp. 73-75

  1. Lankaran State University, Ministry of Education of Azerbaijan, Lankaran, 50 H. Aslanov
  2. Institute Molecular Biology and Biotechnologies of National Academy of Sciences of Azerbaijan, AZ1073, Baku, 11 Izzat Nabiyev
  3. Center for Cell Pathology Research, Department of Life Sciences, Khazar University, Azerbaijan

E-mail: javid.ojaghi khazar.org

Huseynov M., Suleymanova Z., Ojaghi J., Mammadov A. Characterization and phylogeny analysis of Azerbaijan tea (Camellia sinensis L.) genotypes by molecular markers, Tsitol Genet., 2022, vol. 56, no. 3, pp. 73-75.

In "Cytology and Genetics":
M. Huseynov, Z. Suleymanova, J. Ojaghi & A. Mammadov Characterization and Phylogeny Analysis of Azerbaijan Tea (Camellia sinensis L.) Genotypes by Molecular Markers, Cytol Genet., 2022, vol. 56, no. 3, pp. 285291
DOI: 10.3103/S0095452722030057


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