ISSN 0564-3783  

Main page
Information to authors
Editorial board
Mobile version

In Ukrainian

Export citations

Gamma rays induced Cytomictic Variations in Pollen Mother Cells of Cumin (Cuminum cyminum) L.

Kumar G., Bhardwaj M.


Cytomixis is an enigmatic cytological phenomenon found between proximately situated PMCs reported in Cuminum cyminum L. The transmigration of chromatin material between two adjacent meiocytes was observed at various exposure rate of gamma radiation viz., 100, 125, 150 and 175Gy in GC4 variety of cumin. The microsporogenesis study of gamma treated sets reveals intercellular migration through various channels or direct fusion. In the present study, the cytomixis was more frequently noticed at meiosis I as compared to later stage i.e., meiosis II. The rate of recurrence of cytomixis shows gradual increment along with the increasing dose rate of gamma rays. As consequences of cytomixis various aberrant post meiotic products were recorded at later phase of cell cycle. . Moreover, in addition to cytomixis other chromosomal abnormalities were also recorded which leads to reduction in pollen fertility. Syncytes were observed at low frequency but have high evolutionary significance as they produce gametes with higher ploidy level. The production of gametes with unbalanced chromosomes can be further exploited in breeding techniques as they are potent variation inducer.

Key words: Cytomixis, cytoplasmic connection, Cuminum cyminum, Gamma rays, Hyperploid, Hypoploid, Syncytes

Tsitologiya i Genetika 2022, vol. 56, no. 3, pp. 70-72

  • Plant Genetics Laboratory, Department of Botany, University of Allahabad, India

E-mail: mohinibhardwaj2811

Kumar G., Bhardwaj M. Gamma rays induced Cytomictic Variations in Pollen Mother Cells of Cumin (Cuminum cyminum) L., Tsitol Genet., 2022, vol. 56, no. 3, pp. 70-72.

In "Cytology and Genetics":
Girjesh Kumar & Mohini Bhardwaj Gamma Rays Induced Cytomictic Variations in Pollen Mother Cells of Cumin (Cuminum cyminum) L., Cytol Genet., 2022, vol. 56, no. 3, pp. 277284
DOI: 10.3103/S0095452722030069


Bellucci, M., Roscini, C., and Mariani, A., Cytomixis in the pollen mother cells of Medicago sativa L., J. Hered., 2003, vol. 94, no. 6, pp. 512516.

Bhat, T.A., Parveen, S., and Khan, A.H., MMS-induced cytomixis in pollen mother cells of broad bean (Vicia faba L.), Turk. J. Bot., 2006, vol. 30, pp. 273279.

Bione, N.C.P., Pagliarini, M.S., and de Toleredo, J.F.F., Meiotic behavior of several Brazilian soybean varieties, Genet. Mol. Biol., 2000, vol. 23, no. 3, pp. 623631.

Boldrini, K.R., Pagliarini, M.S., and Valle, C.B., Cell fusion and cytomixis during microsporogenesis in Brachiaria humidicola (Poaceae), S. Afr. J. Bot., 2006, vol. 72, pp. 478481.

Datta, A.K., Mukherjee, M., and Iqbal, M., Persistent cytomixis in Ocimum basilicum L. (Lamiaceae) and Withania somnifera (L.) Dun (Solanaceae), Cytologia, 2005, vol. 70, no. 3, pp. 309313.

Dwivedi, H. and Kumar, G., Induced syncyte formation via cytomixis in Trachyspermum ammi (L.) Sprague (Apiaceae), Caryologia, 2018, vol. 71, no. 4, pp. 420427.

Ghaffari, S.M., Occurrence of diploid and polyploidy microspores in Sorghum bicolor (Poaceae) is the result of cytomixis, Afr. J. Biotechnol., 2006, vol. 5, pp. 14501453.

Guan, J.Z., Wang, J.J., Cheng, Z.H., and Li, Z.Y., Cytomixis and meiotic abnormalities during microsporogenesis are responsible for male sterility and chromosome variations in Houttuynia cordata, Genet. Mol. Res., 2012, vol. 11, pp. 121130.

Guo, G.Q. and Zheng, G.C., Hypothesis for functions of intercellular bridges in male germ cell development and its cellular mechanisms, J. Theor. Biol., 2004, vol. 229, no. 1, pp. 139146.

Haroun, S.A., Al Shehr, A.M., and Al Wadie, H.M., Cytomixis in the microsporogenesis of Vicia faba L. (Fabaceae), Cytologia, 2004, vol. 69, pp. 711.

Kornicke, M., Uber ortsveranderung von Zellkarnern SB, Niederhein Ges Natur-U Heilkunde, 1901, pp. 1425.

Kravets, E.A., Cellular and tissue mechanisms of recovery processes in Hordeum distichum L. under irradiation, Cytol. Genet., 2009, vol. 43, pp. 917.

Kravets, E.A., Nature, significance, and cytological consequences of cytomixis, Cytol. Genet., 2012, vol. 46, pp. 188195.

Kravets, E.A., Cytomixis as a primary form of sexual process, Adv. Cytol. Pathol., 2018, vol. 3, no. 4, pp. 8891.

Kumar, P., Singhal, V.K., Cytology of Caltha palustris L. (Ranunculaceae) from cold regions of Western Himalayas, Cytologia, 2008, vol. 73, pp. 137143.

Kumar, G., and Singh, S., Induced cytomictic crosstalk behaviour among micro-meiocytes of Cyamopsis tetragonoloba (L.) Taub.(cluster bean): Reasons and repercussions, CARYAB, 2020, vol. 73, no. 2, pp. 111119.

Kumar, P., Singhal, V.K., Male meiosis, morphometric analysis and distribution pattern of 2× and 4× cytotypes of Ranunculus hirtellus Royle, 1834 (Ranunculaceae) from the cold regions of northwest Himalayas (India), Comp. Cytogenet., 2011, vol. 5, pp. 143161.

Kunakh, V.A., Ontogenetic plasticity of the genome as the basis of plant adaptability, Zhebrakovskie Chteniya III (3d Int. Sci. Conf. Zhebrakov readings), Minsk: Pravo Ekon., 2011.

Larrosa, F.H., Maune, J.F., Erazzu, L.E., and Camadro, E.L., Meiotic abnormalities underlying pollen sterility in wild potato hybrids and spontaneous populations, Plant Biol., 2012, vol. 14, pp. 223233.

Lattoo, S.K., Khan, S., Bamotra, S., and Dhar, A.K., Cytomixis impairs meiosis and influences reproductive success in Chlorophytum comosum (Thunb.) Jacq.An additional strategy and possible implications, J. Biosci., 2006, vol. 31, pp. 629637.

Liu, H., Guo, G.Q., He, Y.K., Lu, Y.P., and Zheng, G.C., Visualization on intercellular movement of chromatin in intact living anthers of transgenic tobacco expressing histone 2BCFP fusion protein, Caryologia, 2007, vol. 60, nos. 12, pp. 120.

Lone, F.A. and Wafai, B.A., Chromsome conspectus and cytogenetic appraisal of some commercial cultivars of cherry (Prunus avium L., P. cerasus L.) and plum (P. domestica L.) using a partially modified staining technique, Phytomorphology, 2009, vol. 59, no. 1, pp. 2934.

Malallah, G.A. and Talaat, A.A., Cytomixis and its possible evolutionary role in a Kuwaiti population of Diplotaxis harra (Brassicaceae), Bot. J. Linn. Soc., 2003, vol. 143, pp. 169175.

Malallah, G.A., Cytomixis and Its Possible Evolutionary Role in a Kuwaiti Population of Diplotaxis harra (Brassicaceae), Comp. Cytogenet., 2011, vol. 5, pp. 143161.

Mamun, E.A., Alfred, S., Cantrill, L.C., Overall, R.L., and Sutton, B.G., Effects of chilling on male gametophyte development in rice, Cell Biol. Int., 2006, vol. 30, no. 7, pp. 583591.

Mursalimov, S.R. and Deineko, E.V., An ultrastructural study of microsporogenesis in tobacco line SR1, Biologia, 2012, vol. 67, pp. 369376.

Mursalimov, S., Sidorchuk, Y., and Deineko, E., The role of spherosome like vesicles information of cytomictic channels between tobacco microsporocytes, Biol. Plant., 2013, vol. 57, pp. 291297.

Mursalimov, S.R. and Deineko, E.V., How cytomixis can form unreduced gametes in tobacco, Plant Syst. Evol., 2015, vol. 301, pp. 12931297.

Nirmala, C. and Kaul, M.L.H., Male sterility in pea VI. Gene action duplicity, Cytologia, 1994, vol. 59, pp. 195201.

Pagliarini, M.S., Meiotic behaviour of economically important plant species: the relationship between fertility and male sterility, Genet. Mol. Biol., 2000, vol. 23, pp. 9971002.

Papihin, R.V. and Yandovka, L.F., Cytomixis during microsporogenesis in Cerasus fruticosa Pall, Biomed. Pharmacol. J., 2014, vol. 7, pp. 473479.

Peng, Z.H.S., Yang, J. and Zheng, G.C.H., Cytomixis in pollen mother cells of new synthetic hexaploid amphidiploid (Aegilops tauschii, Triticum turgidum), Cytologia, 2003, vol. 68, pp. 335340.

Popova, A.F., Ivanenko, G.F., Ustinova, A.Yu., and Zaslavsky, V.A., Localization of callose in microspores and pollen grains in Sium latifolium L. plants in different water regimes, Cytol. Genet., 2008, vol. 42, pp. 363368.

Ramanna, M.S. and Jacobsen, E., Relevance of sexual polyploidization for crop improvementA review, Euphytica, 2003, vol. 133, pp. 318.

Ramsey, J. and Schemske, D.W., Neopolyploidy in flowering plants, Annu. Rev. Ecol. Syst., 2002, vol. 33, pp. 589639.

Ranjbar, M., Karamian, R., and Nouri, S., Impact of cytomixis on meiosis in Astragalus cyclophyllos Beck (Fabaceae) from Iran, Caryologia, 2011, vol. 64, no. 3, pp. 256264.

Reis, A.C., Sousa, S.M., Vale, A.A., Pierre, P.M.O., Franco, A.L., Campos, J.M.S., Viera, R.F., and Viccini, L.F., Lippia alba (Verbenaceae): A new tropical autopolyploid complex?, Am. J. Bot., 2014, vol. 101, pp. 10021012.

Saraswathy Amma, C.K., Namboodiri, A.N., Panikkar, A.O.N., and Sethuraj, M.R., Radiation induced male sterility in Hevea brasiliensis (Willd. ex Adr. De Juss.) Muell. Arg, Cytologia, 1990, vol. 55, no. 4, pp. 547551.

Shkutina, F.M. and Kozlovskaya, V.F., Cytomixis in meiosis of some hybrid forms of cereals of the Triticinae subtribe, Genetics, 1974, vol. 10, pp. 512.

Sidorchuk, Yu.V., Deineko, E.V., and Shumnyi, V.K., Characteristics of the cytomixis in the pollen mother cells of transgenic tobacco plants (Nicotiana tabacum L.) with mutant phenotype, Tsitologya, 2007, vol. 49, no. 10, pp. 870875.

Singhal, V.K., Gill, B.S., and Dhaliwal, R.S., Status of chromosomal diversity in the hardwood tree species of Punjab State, Cytol. Genet., 2007, vol. 8, pp. 6783.

Singhal, V.K. and Kumar, P., Impact of cytomixis on meiosis, pollen viability and pollen size in wild populations of Himalayan poppy (Meconopsis aculeate Royle), J. Biosci., 2008, vol. 33, pp. 371380.

Singhal, V.K., Kaur, S., and Kumar, P., Aberrant male meiosis, pollen sterility and variable sized pollen grains in Clematis Montana Buch.-Ham. ex DC. from Dalhousie hills, Himachal Pradesh, Cytologia, 2010, vol. 75, no. 1, pp. 3136.

Singhal, V.K., Gill, B.S., and Dhaliwal, R.S., Status of chromosomal diversity in the hardwood tree species of Punjab State, Cytol. Genet., 2007, vol. 8, pp. 6783.

Soltis, D.E., Pires, J.C., Kovarik, A., Tate, J., and Mavrodiev, E., Recent and recurrent polyploidy in Tragopogon (Asteraceae): cytogenetic, genomic and genetic comparisons, Biol. J. Linn. Soc., 2004, vol. 82, no. 4, pp. 485501.

Wang, S.Yu., Yu, Ch.H., Li, S., Wang, Ch.Y., and Zheng, G.C., Ultrastructural aspects and possible origin of cytoplasmic channels providing intercellular connection in vegetative tissues of anthers, Russ. J. Plant Physiol., 2004, vol. 51, pp. 97106.

Yu, C.H., Guo, G.Q., Nie, X.W., and Zheng, G.C., Cytochemical localization of pectinase activity in pollen mother cells of tobacco during meiotic prophase I and its relation to the formation of secondary plasmodesmata and cytoplasmic channels, Acta Bot. Sin., 2004, vol. 46, no. 12, pp.143145.

Zhou, S.Q., Viewing the difference between the diploid and the polyploid in the light of the upland cotton aneuploid, Hereditas, 2003, vol. 138, pp. 6572.

Copyright© ICBGE 2002-2023 Coded & Designed by Volodymyr Duplij Modified 29.05.23