ISSN 0564-3783  



Main page
Contacts
Themes
Archive  
Themes
Subscription
Information to authors
Editorial board
Mobile version


In Ukrainian

Export citations
UNIMARC
BibTeX
RIS





Analysis of the relative average long telomere of womens leukocytes, who were sick with COVID-19

Krasnienkov D.S., Gorodna O.V., Kaminska T.M., Podolskiy V.V., Podolskiy Vl.V., Nechyporenko .V., Antypkin Yu.G., Livshits L.A.

 




SUMMARY. Coronavirus disease (COVID-19) is an acute infectious disease of the respiratory tract, the causative agent of which is the new coronavirus SARS-CoV-2.The global vaccination program against SARS-CoV-2 is still on-going, and the incidence of COVID-19 worldwide is significantly decreasing. However, among the millions of survivors of COVID-19, large groups will require assistance due to increased clinical consequences fol-lowing COVID-19. Currently, there is a need to find molecular biomarkers to monitor the occurrence and progression of the post-covid syndrome. For this pur-pose, we were measuring the relative average length of telomeres in groups of women of reproductive age: in a group of patients (n = 64) who contracted COVID-19 and in a control group (n = 42) of women of the same age. The analysis was performed by monochrome quantitative PCR in real time on DNA samples isolated from peripheral blood leukocytes. According to the studys results, it was established that the relative average length of telomeres in peripheral blood leukocytes in the group of patients with COVID-19 was lower than in the control group (p < 0.05). Our results suggest that the observed shortening of the relative average length of telomeres in a group of patients infected with COVID-19 may be evidence in favor of the fact that SARS-CoV-2 infection, may directly cause erosion of telomeres in blood cells, in particular, in leukocytes. Accordingly, the determination of the relative average length of telomeres can be an informative prognostic marker for assessing the risk of the severity of the course of the disease in COVID-19 and the development of the post-covid syndrome.

Key words: COVID-19, biomarker, relative average telomere length, women of reproductive age

Tsitologiya i Genetika 2022, vol. 56, no. 6, pp. 57-62

  1. State Institution Institute of Gerontology named after Dmitry F. Chebotarev, National Academy of Medical Sciences of Ukraine
  2. Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine
  3. Kyiv City Childrens Clinical Hospital of Infectious Diseases
  4. State Institution Institute of Pediatrics, Obstetrics and Gynecology named after Academician O.M. Lukyanova, National Academy of Medical Sciences of Ukraine

E-mail: livshits edu.imbg.org.ua

Krasnienkov D.S., Gorodna O.V., Kaminska T.M., Podolskiy V.V., Podolskiy Vl.V., Nechyporenko .V., Antypkin Yu.G., Livshits L.A. Analysis of the relative average long telomere of womens leukocytes, who were sick with COVID-19, Tsitol Genet., 2022, vol. 56, no. 6, pp. 57-62.

In "Cytology and Genetics":
D. S. Krasnienkov, O. V. Gorodna, T. M. Kaminska, V. V. Podolskiy, Vl. V. Podolskiy, M. V. Nechyporenko, Yu. G. Antypkin & L. A. Livshits Analysis of Relative Average Length of Telomeres in Leukocytes of Women with COVID-19, Cytol Genet., 2022, vol. 56, no. 6, pp. 526529
DOI: 10.3103/S0095452722060056


References

Albrecht, E., Sillanpää, E., Karrasch, S., Alves, A.C., Codd, V., Hovatta, I., Buxton, J.L., Nelson, C.P., Broer, L., Hägg, S., Mangino, M., Willemsen, G., Surakka, I., Ferreira, M.A., Amin, N., Oostra, B.A., Bäckmand, H.M., Peltonen, M., Sarna, S., Rantanen, T., Sipilä, S., Korhonen, T., Madden, P.A., Gieger, C., Jörres, R.A., Heinrich, J., Behr, J., Huber, R.M., Peters, A., Strauch, K., Wichmann, H.E., Waldenberger, M., Blakemore, A.I., de Geus, E.J., Nyholt, D.R., Henders, A.K., Piirilä, P.L., Rissanen, A., Magnusson, P.K., Viñuela, A., Pietiläinen, K.H., Martin, N.G., Pedersen, N.L., Boomsma, D.I., Spector, T.D., van Duijn, C.M., Kaprio, J., Samani, N.J., Jarvelin, M.R., Schulz, H., Telomere length in circulating leukocytes is associated with lung function and disease, Eur. Respir. J., 2014, vol. 43, no. 4, pp. 983992. https://doi.org/10.1183/09031936.00046213

Anderson, J.J., Susser, E., Arbeev, K.G., Yashin, A.I., Levy, D., Verhulst, S., and Aviv, A., Telomere-length dependent T-cell clonal expansion: A model linking ageing to COVID-19 T-cell lymphopenia and mortality, EbioMedicine, 2022, vol. 78, p. 103978. https://doi.org/10.1016/j.ebiom.2022.103978

Aviv, A., Short telomeres and severe COVID-19: The connection conundrum, EbioMedicine, 2021, vol. 70, p. 103513.

Benetos, À., Lai, T.-P., Toupance, S., Labat, C., Verhulst, S., Gautier, S., Ungeheuer, M.-N., Perret-Guillaume, Ch., Levy, D., Susser, E., and Aviv, A., The nexus between telomere length and lymphocyte count in seniors hospitalized with COVID-19, J. Gerontol., Ser. A, 2021, vol. 76, no. 8, pp. e97e101. https://doi.org/10.1093/erona/glab026

Cawthon, R.M., Telomere length measurement by a novel monochrome multiplex quantitative PCR method, Nucleic Acids Res., 2009, vol. 37, no. 3, p. e21.

Dos Santos, G.A., Pimenta, R., Viana, N.I., Guimarães, V.R., Romão, P., Candido, P., de Camargo, J.A., Hatanaka, D.M., Queiroz, P.G., Teruya, A., Leite, K.R.M., Srougi, V., Srougi, M., and Reis, S.T., Shorter leukocyte telomere length is associated with severity of COVID-19 infection, Biochem. Biophys. Rep., 2021, vol. 27, p. 101056. https://doi.org/10.1016/j.bbrep.2021.101056

Froidure, A., Mahieu, M., Hoton, D., Laterre, P.-F., Yombi, J.C., Koenig, S., Ghaye, B., Defour, J.-P., and Decottignies, A., Short telomeres increase the risk of severe COVID-19, Aging, 2020, vol. 12, no. 20, pp. 1991119922.

Guescini, M., Sisti, D., Rocchi, M.B., Stocchi, L., and Stocchi, V., A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition, BMC Bioinformatics, 2008, vol. 30, no. 9, p. 326. https://doi.org/10.1186/1471-2105-9-326

Horvath, S. and Levine, A.J., HIV-1 Infection accelerates age according to the epigenetic clock, J. Infect. Dis., 2015, vol. 212, pp.15631573.

Jiang, L., Tang, B.S., Guo, J.F., and Li, J.C., Telomere length and COVID-19 outcomes: A two-sample bidirectional mendelian randomization study, Front. Genet., 2022, vol. 23, no. 13, p. 805903. https://doi.org/10.3389/fgene.2022.805903

Kananen, L., Nevalainen, T., Jylhävä, J., Marttila, S., Hervonen, A., Jylhä, M., and Hurme, M., Cytomegalovirus infection accelerates epigenetic aging, Exp. Gerontol., 2015, vol. 72, pp. 227229.

Khalangot, M.D., Krasnienkov, D.S., and Vaiserman, A.M., Telomere length in different metabolic categories: Clinical associations and modification potential, Exp. Biol. Med., 2020, vol. 245, no. 13, pp. 11151121.

Magaña-Acosta, M. and Valadez-Graham, V., Chromatin remodelers in the 3D nuclear compartment, Front. Genet., 2020, vol. 11, p. 600615. https://doi.org/10.3389/fgene.2020.600615

Mongelli, A., Barbi, V., Zamperla, M.G., et al., Evidence for Biological Age Acceleration and Telomere Shortening in COVID-19 Survivors, Int. J. Mol. Sci., 2021, vol. 22, no. 11, p. 6151. https://doi.org/10.3390/ijms22116151

Oronsky, B., Larson, C., Hammond, T.C., Oronsky, A., Kesari, S., Lybeck, M., and Reid T.R., A Review of Persistent Post-COVID Syndrome (PPCS), Clin. Rev. Allergy Immunol., 2021, vol. 20, pp. 19. https://doi.org/10.1007/s12016-021-08848-3

Ren, L.L., Wang, Y.M., Wu, Z.Q., et al., Identification of novel coronavirus causing severe pneumonia in human: a descriptive study, Chin. Med. J., 2020, vol. 133, no. 9, pp. 10151024. https://doi.org/10.1097/CM9.0000000000000722

Sabry, D., Ahmed, R., Abdalla, S., Fathy, W., Eldemery, A., Elamir, A., Braf, Kras and Helicobacter pylori epigenetic changes-associated chronic gastritis in Egyptian patients with and without gastric cancer, World J. Microbiol. Biotechnol., 2016, vol. 32, p. 92.

Sanchez-Vazquez, R., Guío-Carrión, A., Zapatero-Gaviria, A., Martínez, P., and Blasco, M.A., Shorter telomere lengths in patients with severe COVID-19 disease, Aging (Albany), 2021, vol. 13, no. 1, pp. 115. https://doi.org/10.18632/aging.202463

Tyagi, M., Imam, N., Verma, K., Patel, A.K., Chromatin remodelers: We are the drivers!!, Nucleus, 2016, vol. 7, no. 4, pp. 388404.https://doi.org/10.1080/19491034.2016.1211217

Velavan, T.P. and Meyer, C.G., The COVID-19 epidemic, Trop. Med. Int. Health, 2020, vol. 25, no. 3, pp. 278280.https://doi.org/10.1111/tmi.13383

Walitt, B. and Bartrum, E., A clinical primer for the expected and potential post-COVID-19 syndromes, Pain Rep., 2021, vol. 6, p. 887.

Copyright© ICBGE 2002-2023 Coded & Designed by Volodymyr Duplij Modified 05.06.23