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The role of nitric oxide in the Arabidopsis thaliana response to simulated microgravitation and the participation of autophagy in the mediation of this process

Plokhovska S.H., Shadrina R.Yu., Kravets O.A., Yemets A.I., Blume Ya.B.

 




SUMMARY.The role of nitric oxide (NO) in the response of plants to simulated microgravity has been studied. It was found that treatment of Arabidopsis thaliana seeds with NO donor sodium nitroprusside (SNP), stimulating changes in root growth parameters, and leads to increased plant resistance to clinostating. After treatment of SNP seeds, the content of endogenous NO in control plants on the 6th day of cultivation increased 1.5 times, and in clinostated 1.8 times. The content of endogenous NO gradually decreased on 912 days which may indicate the adaptation of plants to the conditions of clinostating. Using a specific DAF-FM DA fluorescent probe, an increase in NO fluorescence was found in epidermal cells of root apexes and root hairs in clinostated plants, indicating the accumulation of endogenous NO in these root tissues under stress. On the 6 day of cultivation under clinostat condition compared with control plants an increase in the accumulation of autophagosomes in the epidermal cells of the transitional zone of the root with the following decrease in this indicator on 912 days. Seed treatment with cPTIO (NO scavenger) slightly inhibited seedling growth, and this effect was enhanced under clinostation, including a significant increase in the accumulation of autophagosomes in epidermal cells. Thus, the data obtained indicate that the regulation of endogenous NO content is an important component of intracellular signaling mechanisms that are involved in the response of plant cells to simulated microgravity.

Key words: simulated microgravity, clinostation, nitric oxide (NO), NO donors, sodium nitroprusside (SNP), NO scavengers, cPTIO, autophagy

Tsitologiya i Genetika 2022, vol. 56, no. 3, pp. 44-54

  • Institute of Food Biotechnology and Genomics, NAS of Ukraine, Osypovskogo str., 2a, Kyiv 04123, Ukraine

E-mail: blume.yaroslav nas.gov.ua

Plokhovska S.H., Shadrina R.Yu., Kravets O.A., Yemets A.I., Blume Ya.B. The role of nitric oxide in the Arabidopsis thaliana response to simulated microgravitation and the participation of autophagy in the mediation of this process, Tsitol Genet., 2022, vol. 56, no. 3, pp. 44-54.

In "Cytology and Genetics":
S. H. Plokhovska, R. Yu. Shadrina, O. A. Kravets, A. I. Yemets & Ya. B. Blume The Role of Nitric Oxide in the Arabidopsis thaliana Response to Simulated Microgravity and the Involvement of Autophagy in This Process, Cytol Genet., 2022, vol. 56, no. 3, pp. 244252
DOI: 10.3103/S0095452722030100


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