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Associations between leptin gene and leptin receptor polymorphisms and the meat quality of ukrainian large white pigs

Balatsky V.M., Oliynychenko Ye.K., Saienko A.M., Buslyk T.V., Bankovska I.B., Peka M.Yu., Doran O.

 




SUMMARY. The associations between SNP in leptin gene (LEP g.2845A>T, LEP g.3996T>C, LEP g.3469T>C) and lep-tin receptor gene (LEPR c.232A>T, LEPR c.2856C>T, LEPR c.915C>T) and the meat quality traits of Ukrainian Large White pigs (ULW) were studied. The computational analysis (SIFT, PolyPhen-2 and I-Mutant bioinformational services) was conducted to investigate the effect of mutations, causing SNP, for both selected SNP, and those missense SNP, for which they can be potential linkage disequilibrium (LD) markers, on the structure and functions of leptin and leptin receptor. LEP SNP c.3469T>C (exon 3, rs45431504) is associated with the protein content in meat and the content of moisture in the back fat. LEP SNP g.2845A>T (rs344615147), which is associated with moisture-retaining ability of meat and the content of moisture in the back fat, is located in the second intron and, similar to SNP c.3469T>C, may be viewed as an LD marker of missense-polymorphism of SNP LEP rs701423985. LEPR SNP c.232A>T (exon 4, rs45435517) is a missense mutation, causing the amino acid replacement S52I. The evaluations, made using SIFT and PolyPhen-2, demonstrate a considerable impact of rs45435517 on the functional characteristics of leptin receptor. Our study determined the association between SNP c.232A>T and fat thickness. At the same time, SNP c.2856C>T (exon 20, rs694660564, a synonymic replacement), which in our work demonstrated the association with the loss of moisture in meat for ULW pigs, may be a potential LD marker and be in disequilibrium with the linkage to missense SNP LEPR rs1113972516, rs792804682 and rs1109261799. SNP, for which in our work the associations were found with meat quality traits, may be viewed as potential markers for the breeding of ULW pigs and possibly other breeds, directed at obtaining genotypes with improved characteristics of meat quality.

Key words: pigs, leptin gene, leptin receptor gene, SNP, meat quality traits, association, computational analysis of SNP effects

Tsitologiya i Genetika 2022, vol. 56, no. 6, pp. 42-56

  1. Genetics Laboratory, the Institute of Pig Breeding and Agroindustrial Production, the National Academy of Agrarian Sciences of Ukraine, Shvedska Mohyla, 1, Poltava, 36013, Ukraine
  2. V.N. Karazin Kharkiv National University, Svobody square, 4, Kharkiv, 61022, Ukraine
  3. Laboratory of Metabolism named after Stepan Gzhytskyi, the Institute of Animal Biology, the National Academy of Agrarian Sciences of Ukraine, V. Stusa Str., 38 Lviv, 79034, Ukraine
  4. Faculty of Health and Applied Sciences, the University of the West of England, Frenchay Campus, Coldharbour Lane, Bristol, BS16 1 QY, UK

E-mail: vnbalatsky gmail.com, olejliza gmail.com, artsm82 gmail.com, tvbuslyk gmail.com, gloryir2017 gmail.com, pekapoltava gmail.com, olena.doran uwe.ac.uk

Balatsky V.M., Oliynychenko Ye.K., Saienko A.M., Buslyk T.V., Bankovska I.B., Peka M.Yu., Doran O. Associations between leptin gene and leptin receptor polymorphisms and the meat quality of ukrainian large white pigs, Tsitol Genet., 2022, vol. 56, no. 6, pp. 42-56.

In "Cytology and Genetics":
V. N. Balatsky, Y. K. Oliinychenko, A. M. Saienko, T. V. Buslyk, I. B. Bankovska, M. Yu. Peka & O. Doran Associations of Polymorphisms in Leptin and Leptin Receptor Genes with Meat Quality in Pigs of the Ukrainian Large White Breed, Cytol Genet., 2022, vol. 56, no. 6, pp. 513525
DOI: 10.3103/S0095452722060020


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