Information to authors
Relationship of stimulation of plant antioxidant protection and signs of genome instability
SUMMARY. The relationship between stimulation of low-molecular-weight antioxidant accumulation in pharmaceutical plant raw material (inflorescences) and the markers of radiation-induced genome instability at the stage of plant flowering under X-ray exposure were investigated. The study of rearrangements of DNA primary structure under different dose exposure was carried out by PCR using eight ISSR and ten RAPD primers. Dose – dependent changes in amplicon spectra during ISSR – RAPD – PCR were analyzed using the Jacquard similarity index. It was found that the largest rearrangements of the primary DNA structure of both genotypes, which was indicated as a decrease in similarity with the control spectra of amplicon, was observed under exposure with doses 5–10 Gray. There was a tendency to approach this indicator to the control one under 15 Gray dose, which mint increased efficiency of reparative processes. The relationship between the polymorphism of the primary structure of DNA by ISSR-RAPD-sequences through different genotypes and the nature of its rearrangement under radiation exposure was shown. Comparison of the results with no monotonic dose curves of the specific flavonoids’ and phenols’ content allowed us to conclude that the stimulation of antioxidant protection was shown under doses corresponding to low efficiency of repair processes and, accordingly reduced it under genetic material repair. The interpretation of the identified phenomenon is based on the known connection between the effects of genomic instability and the increase in the level of reactive oxygen species and the general principles of antioxidant protection. The significance of the obtained results through the development of the scientific basis for the implementation of small radiation exposure doses in biotechnology, particularly in pharmacology is discussed.
Key words: genome instability, pre-sowing seeds radiation exposure, secondary metabolism, biotechnology
E-mail: dasokolova88 gmail.com, vzhukv gmail.com, sdgfbd gmail.com, kaplibra gmail.com, nkuchuk icbge.org.ua
Aguilera, A. and García-Muse, T., Causes of genome instability, Ann. Rev. Genet., 2013, vol. 47, pp. 1–32. https://doi.org/10.1146/annurev-genet-111212-133232
Àlothman, M., Bhat, R., and Karim, A.A., Effects of radiation processing on phytochemicals and antioxidants in plant produce, Trends Food Sci. Technol., 2009, vol. 20, no. 5, pp. 201–212. https://doi.org/10.1016/j.tifs.2009.02.003
Aypar, U., Morgan, W.F., and Baulch, J.E., Radiation-induced genomic instability: Are epigenetic mechanisms the missing link?, Int. J. Radiat. Biol., 2010, vol. 87, no. 2, pp. 179–191. https://doi.org/10.3109/09553002.2010.522686
Clark, D.J., Nucleosome positioning, nucleosome spacing and the nucleosome code, J. Biomol. Struct. Dyn., 2010, vol. 27, pp. 781–793.
Barlow, J.H., et al., Identification of early replicating fragile sites that contribute to genome instability, Cell, 2013, vol. 152, no. 3, pp. 620–632. https://doi.org/10.1016/j.cell.2013.01.006
Burlakova, E.B., et al., Features of the biological effect of small doses of radiation, Rad. Biol. Radioecol., 1999, vol. 39, art. ID 26.
Croft, K.D., The chemistry and biological effects of flavonoids and phenolic acids, Ann. New York Acad. Sci., 1998, vol. 854, no. 1, pp. 435–442. https://doi.org/10.1111/j.1749-6632.1998.tb09922.x
Durkin, S.G. and Glover, T.W., Chromosome fragile sites, Ann. Rev. Gen., 2007, vol. 41, pp. 169–192. https://doi.org/10.1146/annurev.genet.41.042007.165900
Gaziev, A.I., Low efficiency of repair of critical DNA damage caused by low doses of radiation, Rad. Biol. Radioecol., 2011, vol. 51, no. 5, pp. 512–529.
Ellegren, H., Microsatellites: simple sequences with complex evolution, Nat. Rev. Genet., 2004, vol. 5, no. 6, pp. 435–445. https://doi.org/10.1038/nrg1348
Eliseeva, I.I. and Rukavishnikov, V.O., Grouping, Correlation, Pattern Recognition: (Statistical Methods of Classification and Measurement of Relationships), Moscow: Statistics, 1977.
Halliwell, B., Antioxidant defense mechanisms: From the beginning to the end (of the beginning), Free Rad. Res., 1999, vol. 31, pp. 261–72. https://doi.org/10.1080/10715769900300841
Hemleben, V., Beridze, T.G., and Bakhman, L., Satellite DNA, Usp. Biol. Khim., 2003, vol. 43, pp. 267–306.
IAEA. Cytogenetic Analysis for Radiation Dose Assessment. Thechnical reports. Series N 405. 2001. International Atomic Energy Agency, Vienna, STI/DOC/010/ 40592-0-102101-1.
Kim, J.-H., Ryu, T.H., Lee, S.S., et al., Ionizing radiation manifesting DNA damage response in plants: An overview of DNA damage signalling and repair mechanisms in plants, Plant Sci., 2019, vol. 278, pp. 44–53. https://doi.org/10.1016/j.plantsci.2018.10.013
Khattak, K. and Simpson, D., Effect of gamma irradiation on the extraction yield, total phenolic ñîntent and free radical-scavenging activity of Nigella sativa seed, Food Chem., 2008, vol. 110, no. 4, pp. 967–972. https://doi.org/10.1016/j.foodchem.2008.03.003
Kolomĭytseva, I.B., Non-monotonicity of the dose-effect relationship in the region of low doses of ionizing radiation, Rad. Biol. Radioecol., 2003, vol. 43, no. 2, pp. 179–181.
Kravets, A.P. and Sokolova, D., Epigenetic factors of individual radiosensitivity and adaptive capacity, Int. J. Rad. Biol., 2020, vol. 96, no. 8, pp. 999–1007. https://doi.org/10.1080/09553002.2020.1767819
Kumari, S., Rastogi, R.P., and Singh, K.L., DNA damage: Detection strategies, EXCLI J., 2008, vol. 7, pp. 44–62.
Lakin, G.F., Biometrics, Moscow: Higher School, 1990.
López-Flores, I. and Garrido-Ramos, M.A., The repetitive DNA content of eukaryotic genomes, Genome Dyn., 2012, vol. 7, pp. 1–28. https://doi.org/10.1159/000337118
Moghaddam, S., et al., Effects of acute gamma irradiation on physiological traits and flavonoid accumulation of Centella asiatica, Molecules, 2011, vol. 16, no. 6, pp. 4994–5007. https://doi.org/10.3390/molecules16064994
Pinto, M., Prise, K.M., and Michael, B.D., Evidence for complexity at the nanometer scale of radiationinduced DNA DSBs as a determinant of rejoining kinetics, Rad. Res., 2005, vol. 164, no. 1, pp. 73–85. https://doi.org/10.1667/rr3394
Poyraz, I., Comparison of ITS, RAPD and ISSR from DNA-based genetic diversity techniques, C. R. Biol., 2016, vol. 339, nos. 5–6, pp. 171–178.
De Bont, R. and van Larebeke, N., Endogenous DNA damage in humans: a review of quantitative data, Mutagenesis, 2004, vol. 19, no. 3, pp. 169–185. https://doi.org/10.1093/mutage/geh025
Sies, H. and Jones, D.P., Reactive oxygen species (ROS) as pleiotropic physiological signalling agents, Nat. Rev. Mol. Cell Biol., 2020, vol. 21, pp. 363–383. https://doi.org/10.1038/s41580-020-0230-3
Sokolova, D.A., Vengzhen, G.S., and Kravets, A.P., An Analysis of the correlation between the changes in satellite DNA methylation patterns and plant cell responses to the stress, CellBio, 2013, vol. 2, no. 3, pp. 163–171. https://doi.org/10.4236/cellbio.2013.23018
Sokolova, D., Kravets, A., Zhuk, V., Sakada, V., Gluschenko, L., and Kuchuk, M., Productivity of medicinal raw materials by different genotypes of Matricia Chammomila L. is affected with pre-sowing radiation exposure of seeds, Int. J. Second. Metab., 2021, vol. 8, no. 2, pp. 127–135. https://doi.org/10.21448/ijsm.889817
Sumira, J., Parween, T., and Siddiqi, T.O., Effect of gamma radiation on morphological, biochemical, and physiological aspects of plants and plant products, Environ. Rev., 2012, vol. 20, no. 1, pp. 17–39. https://doi.org/10.1139/a11-021
Szumiel, I., Ionizing radiation-induced oxidative stress, epigenetic changes and genomic instability: The pivotal role of mitochondria, Int. J. Radiat. Biol., 2015, vol. 91, no. 1, pp 1–12. https://doi.org/10.3109/09553002.2014.934929
Teĭf, V.B., Shkrobkov, A.V., Egorova, V.P., et al., Nucleosomes in gene regulation: theoretical approach, Mol. Biol., 2012, vol. 46, no. 1, pp. 3–13. https://doi.org/10.1134/S002689331106015X
Teĭf, V.B., Nucleosome positioning: resources and tools online, Briefings Bioinf., 2015, vol. 17, no. 5, pp. 745–757. https://doi.org/10.1093/bib/bbv086
Tominaga, H., Kodama, S., Matsuda, N., et al., Involvement of reactive oxygen species (ROS) in the induction of genetic instability by radiation, J. Radiat. Res., 2004, vol. 45, no. 2, pp. 181–188. https://doi.org/10.1269/jrr.45.181
Tubiana, M., Aurengo, A., and Averbeck, D., Recent reports on the effect of low doses of ionizing radiation and its dose–effect relationship, Rad. Environ. Biophys., 2006, vol. 44, art. ID 245. https://doi.org/10.1007/s00411-006-0032-9
Winkel-Shirley, B., Biosynthesis of flavonoids and effects of stress, Curr. Opin. Plant Biol., 2002, vol. 5, no. 3, pp. 218–223. https://doi.org/10.1016/s1369-5266(02)00256-x
Zhuk, V., Sokolova, D., Kravets, A., et al., Efficiency of pre-sowing seeds by UV-C and X-ray exposure on the accumulation of antioxidants in inflorescence of plants of Matricaria chamomilla L. genotypes, Int. J. Sec. Metabol., 2021, vol. 8, no. 3, pp. 186–194. https://doi.org/10.21448/ijsm.889860
|Coded & Designed by Volodymyr Duplij||Modified 05.12.23|