TSitologiya i Genetika 2022, vol. 56, no. 4, 39-47
Cytology and Genetics 2022, vol. 56, no. 4, 343–350, doi: https://www.doi.org/10.3103/S0095452722040053

Preincubation of L929 line fibroblasts with synthetic leu-enkephalin Tyr-D-Ala-Gly-Phe-Leu-Arg (Dalargin) preserves their proliferative potential under cold stress

Gulevskyy О.К., Moisieieva N.М., Gorina О.L., Sidorenko О.S.

  • Institute for Problems of Cryobiology and Cryomedicine of the National Academy
    of Sciences of Ukraine, Perejaslavska str., 23,
    Kharkiv, 61016, Ukraine

SUMMARY. The effect of synthetic leu-enkephalin on apoptosis, necrosis and proliferative properties of fibroblasts L929 cell line in the monolayer without damage and with a «wound surface» under cold stress was studied. It is proved that cold stress initiates apoptosis, necrosis and slows down cell proliferation in the damaged and undamaged monolayer of L929 fibroblasts. Preincubation of cells with synthetic leu-enkephalin before modeling for cold stress leads to a probable (p < 0,05) decrease in the number of fibroblasts with morphological signs of necrosis and apoptosis and a probable (p < 0,05) increase in the proliferative potential of cells in intact monolayer and with a wound surface. It has been found that the protective effect of synthetic leu-enkephalin is dose-dependent and differs significantly under conditions of cold stress during wound healing. The greatest effectiveness of synthetic leu-enkephalin to prevent cell apoptosis and stimulate cell proliferation under cold stress is observed at a concentration of 10 and 100μg/l.

Keywords: cold stress, synthetic leu-enkephalin, L929 fibroblast cell line, apoptosis, necrosis, cell proliferation, wound surface

TSitologiya i Genetika
2022, vol. 56, no. 4, 39-47

Current Issue
Cytology and Genetics
2022, vol. 56, no. 4, 343–350,
doi: 10.3103/S0095452722040053

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