Huet А., Dvorshchenko К., Grebinyk D., Beregova T., Ostapchenko L.

SUMMARY. The decrease in the expression levels of Ctfr and Ocln genes was shown while healing planar full-thickness excisional wounds as well as purulonecrotic skin wounds in rats on the background of the increase in the expression level of Nfkb1. The restoration of Ocln gene expression might be mediated by the increase of Ctfr gene expression caused by the decrease in Nfkb1 mRNA level. When melanin was applied under these conditions, the values of Ctfr and Ocln expression reached the corresponding values for the control group of rats faster without the Nfkb1 hyperexpression during the recovery of skin integrity.

Tsitologiya i Genetika 2022, vol. 56, no. 3, pp. 35-43

• Educational and Scientific Center «Institute of Biology and Medicine» Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Str., Kyiv, 01601

Caldas, M., Cláudia Santos, A., Veigaa, F., et al., Melanin nanoparticles as a promising tool for biomedical applications – a review, Acta Biomaterialia, 2020, vol. 105, pp. 26–43. https://doi.org/10.1016/j.actbio.2020.01.044

Cavallini, C., Vitiello, G., Adinolfi, B., et al., Melanin and melanin-like hybrid materials in regenerative medicine, Nanomaterials, 2020, vol. 10, no. 8, p. 1518. https://doi.org/10.3390/nano10081518

Chen, J., Chen, Y., Chen, Y., et al., Epidermal CFTR suppresses MAPK/NF- κB to promote cutaneous wound healing, Cell. Physiol. Biochem., 2016, vol. 39, no. 6, pp. 2262–2274. https://doi.org/10.1159/000447919

Chomczynski, P. and Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction, Anal. Biochem., 1987, vol. 162, no. 1, pp. 156–159. https://doi.org/10.1006/abio.1987.9999

Costantini, T., Loomis, W., Putnam, J., et al., Burn-induced gut barrier injury is attenuated by phosphodiesterase inhibition: effects on tight junction structural proteins, Shock, 2009, vol. 31, pp. 416–422. https://doi.org/10.1097/SHK.0b013e3181863080

Crites, K., Morin, G., Orlando, V., et al., CFTR knockdown induces proinflammatory changes in intestinal epithelial cells, J. Inflammation, 2015, vol. 12, art. ID 62. https://doi.org/10.1186/s12950-015-0107-y

Cui, Y., Wang, X., Xue, J., et al., Chrysanthemum morifolium extract attenuates high-fat milk-induced fatty liver through peroxisome proliferator-activated receptor α–mediated mechanism in mice, Nutr. Res., 2014, vol. 34, no. 3, pp. 268–275. https://doi.org/10.1016/j.nutres.2013.12.010

De Lisle, R., Disrupted tight junctions in the small intestine of cystic fibrosis mice, Cell Tissue Res., 2014, vol. 355, pp. 131–142. https://doi.org/10.1007/s00441-013-1734-3

Dong, J., Jiang, X., Zhang, X., et al., Dynamically regulated CFTR expression and its functional role in cutaneous wound healing, J. Cell. Physiol., 2015, vol. 230, no. 9, pp. 2049–2058. https://doi.org/10.1002/jcp.24931

Dong, Z., Chen, J., Ruan, Y., et al., CFTR-regulated MAPK/NF-κB signaling in pulmonary inflammation in thermal inhalation injury, Sci. Rep., 2015, vol. 5, art. ID 15946. https://doi.org/10.1038/srep15946

Dranitsina, A., Taburets, O., Dvorshchenko, K., et al., TGFB 1, PTGS 2 genes expression during dynamics of wound healing and with the treatment of melanin, Res. J. Pharm., Biol. Chem. Sci., 2017, vol. 8, no. 1, pp. 2014–2023. https://doi.org/10.3103/S0095452718030039

Golyshkin, D., Falaleeva, T., Neporada, K., and Beregova, T., Effect of melanin on the condition of gastric mucosa and reaction of the hypothalamic-pituitary-adrenal axis under acute stress, Physiol. J., 2015, vol. 61, no. 2, pp. 65–72. https://doi.org/10.15407/fz61.02.065

Heindryckx, F., Binet, F., Ponticos, M., et al., Endoplasmic reticulum stress enhances fibrosis through IRE1α-mediated degradation of miR-150 and XBP-1 splicing, EMBO Mol. Med., 2016, vol. 8, no. 7, pp. 729–744. https://doi.org/10.15252/emmm.201505925

Hsu, H., Liu, C., Lin, J., et al., Involvement of ER stress, PI3K/AKT activation, and lung fibroblast proliferation in bleomycin-induced pulmonary fibrosis, Sci. Rep., 2017, vol. 7, no. 1, art. ID 14272. https://doi.org/10.1038/s41598-017-14612-5

Huet, À., Dvorshchenko, Ê., Taburets, Î., Grebinyk, D., Beregova, T., and Ostapchenko, L., Tlr2 and Tjp1 genes’ expression during restoration of skin integrity, Cyt. Genet., 2020, vol. 54, no. 6, pp. 539–545. https://doi.org/10.3103/S0095452720060122

Kanigur-Sultuybek, G., Yenmis, G., and Soydas, T., Functional variations of NFKB1 and NFKB1A in inflammatory disorders and their implication for therapeutic approaches, Asian Biomed., 2020, vol. 14, no. 2, pp. 47–57. https://doi.org/10.1515/abm-2020-0008

Lee, H. and Jang, Y., Recent understandings of biology, prophylaxis and treatment strategies for hypertrophic scars and keloids, Int. J. Mol. Sci., 2018, vol. 19, no. 3. https://doi.org/10.3390/ijms19030711

Li, W., Wang, C., Peng, X., et al., CFTR inhibits the invasion and growth of esophageal cancer cells by inhibiting the expression of NF-κB, Cell Biol. Int., 2018, vol. 42, no. 12, pp. 1680–1687. https://doi.org/10.1002/cbin.11069

Liu, X., Chen, Y., You, B., et al., Molecular mechanism mediating enteric bacterial translocation after severe burn: the role of cystic fibrosis transmembrane conductance regulator, Burns Trauma, 2021, vol. 9, art. ID tkaa042. https://doi.org/10.1093/burnst/tkaa042

Livak, K. and Schmittgen, T., Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT

method, Methods, 2001, vol. 25, no. 4, pp. 402–408. https://doi.org/10.1006/meth.2001.126210.1006/meth.2001.1262

Orman, M., Nguyen, T., Ierapetritou, M., et al., Comparison of the cytokine and chemokine dynamics of the early inflammatory response in models of burn injury and infection, Cytokine, 2011, vol. 55, no. 3, pp. 362–371. https://doi.org/10.1016/j.cyto.2011.05.010

Sarrazy, V., Billet, F., Micallef, L., et al., Mechanisms of pathological scarring: Role of myofibroblasts and current developments, Wound Repair Regener., 2011, vol. 19, no. s1, pp. 10–15. https://doi.org/10.1111/j.1524-475X.2011.00708.x

Stacey, A., D’Mello, N., Graeme, J., et al., Signaling pathways in melanogenesis, Int. J. Mol. Sci., 2016, vol. 17, no. 7, art. ID 1144. https://doi.org/10.3390/ijms17071144

Yang, A., Sun, Y., Mao, C., et al., Folate protects hepatocytes of hyperhomocysteinemia mice from apoptosis via cystic fibrosis transmembrane conductance regulator (CFTR)-activated endoplasmic reticulum stress, J. Cell. Biochem., 2017, vol. 118, no. 9, pp. 2921–2932. https://doi.org/10.1002/jcb.25946

Zhou, Y., Zhao, Y., Du, H., et al., Downregulation of CFTR is involved in the formation of hypertrophic scars, BioMed. Res. Int., 2020, vol. 2020, art. ID 9526289. https://doi.org/10.1155/2020/9526289