ISSN 0564-3783  

Main page
Information to authors
Editorial board
Mobile version

In Ukrainian

Export citations

Expression of Cftr, Nfkb1 and Ocln genes during the restoration of skin integrity

Huet ., Dvorshchenko ., Grebinyk D., Beregova T., Ostapchenko L.


SUMMARY. The decrease in the expression levels of Ctfr and Ocln genes was shown while healing planar full-thickness excisional wounds as well as purulonecrotic skin wounds in rats on the background of the increase in the expression level of Nfkb1. The restoration of Ocln gene expression might be mediated by the increase of Ctfr gene expression caused by the decrease in Nfkb1 mRNA level. When melanin was applied under these conditions, the values of Ctfr and Ocln expression reached the corresponding values for the control group of rats faster without the Nfkb1 hyperexpression during the recovery of skin integrity.

Tsitologiya i Genetika 2022, vol. 56, no. 3, pp. 35-43

  • Educational and Scientific Center Institute of Biology and Medicine Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Str., Kyiv, 01601

E-mail: alevtina.dranitsina

Huet ., Dvorshchenko ., Grebinyk D., Beregova T., Ostapchenko L. Expression of Cftr
, Nfkb1 and Ocln genes during the restoration of skin integrity, Tsitol Genet., 2022, vol. 56, no. 3, pp. 35-43.

In "Cytology and Genetics":
A. S. Huet, K. O. Dvorshchenko, D. M. Grebinyk, T. V. Beregova & L. I. Ostapchenko Expression of the Cftr, Nfkb1, and Ocln Genes during Restoration of Skin Integrity, Cytol Genet., 2022, vol. 56, no. 3, pp. 236243
DOI: 10.3103/S0095452722030148


Alvim, F. and Addor, S., Antioxidants in dermatology, An. Bras. Dermatol., 2017, vol. 92, no. 3, pp. 356362.

Caldas, M., Cláudia Santos, A., Veigaa, F., et al., Melanin nanoparticles as a promising tool for biomedical applications a review, Acta Biomaterialia, 2020, vol. 105, pp. 2643.

Cavallini, C., Vitiello, G., Adinolfi, B., et al., Melanin and melanin-like hybrid materials in regenerative medicine, Nanomaterials, 2020, vol. 10, no. 8, p. 1518.

Chen, J., Chen, Y., Chen, Y., et al., Epidermal CFTR suppresses MAPK/NF- κB to promote cutaneous wound healing, Cell. Physiol. Biochem., 2016, vol. 39, no. 6, pp. 22622274.

Chomczynski, P. and Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction, Anal. Biochem., 1987, vol. 162, no. 1, pp. 156159.

Costantini, T., Loomis, W., Putnam, J., et al., Burn-induced gut barrier injury is attenuated by phosphodiesterase inhibition: effects on tight junction structural proteins, Shock, 2009, vol. 31, pp. 416422.

Crites, K., Morin, G., Orlando, V., et al., CFTR knockdown induces proinflammatory changes in intestinal epithelial cells, J. Inflammation, 2015, vol. 12, art. ID 62.

Cui, Y., Wang, X., Xue, J., et al., Chrysanthemum morifolium extract attenuates high-fat milk-induced fatty liver through peroxisome proliferator-activated receptor αmediated mechanism in mice, Nutr. Res., 2014, vol. 34, no. 3, pp. 268275.

De Lisle, R., Disrupted tight junctions in the small intestine of cystic fibrosis mice, Cell Tissue Res., 2014, vol. 355, pp. 131142.

Dong, J., Jiang, X., Zhang, X., et al., Dynamically regulated CFTR expression and its functional role in cutaneous wound healing, J. Cell. Physiol., 2015, vol. 230, no. 9, pp. 20492058.

Dong, Z., Chen, J., Ruan, Y., et al., CFTR-regulated MAPK/NF-κB signaling in pulmonary inflammation in thermal inhalation injury, Sci. Rep., 2015, vol. 5, art. ID 15946.

Dranitsina, A., Taburets, O., Dvorshchenko, K., et al., TGFB 1, PTGS 2 genes expression during dynamics of wound healing and with the treatment of melanin, Res. J. Pharm., Biol. Chem. Sci., 2017, vol. 8, no. 1, pp. 20142023.

Golyshkin, D., Falaleeva, T., Neporada, K., and Beregova, T., Effect of melanin on the condition of gastric mucosa and reaction of the hypothalamic-pituitary-adrenal axis under acute stress, Physiol. J., 2015, vol. 61, no. 2, pp. 6572.

Heindryckx, F., Binet, F., Ponticos, M., et al., Endoplasmic reticulum stress enhances fibrosis through IRE1α-mediated degradation of miR-150 and XBP-1 splicing, EMBO Mol. Med., 2016, vol. 8, no. 7, pp. 729744.

Hsu, H., Liu, C., Lin, J., et al., Involvement of ER stress, PI3K/AKT activation, and lung fibroblast proliferation in bleomycin-induced pulmonary fibrosis, Sci. Rep., 2017, vol. 7, no. 1, art. ID 14272.

Huet, À., Dvorshchenko, Ê., Taburets, Î., Grebinyk, D., Beregova, T., and Ostapchenko, L., Tlr2 and Tjp1 genes expression during restoration of skin integrity, Cyt. Genet., 2020, vol. 54, no. 6, pp. 539545.

Kanigur-Sultuybek, G., Yenmis, G., and Soydas, T., Functional variations of NFKB1 and NFKB1A in inflammatory disorders and their implication for therapeutic approaches, Asian Biomed., 2020, vol. 14, no. 2, pp. 4757.

Lee, H. and Jang, Y., Recent understandings of biology, prophylaxis and treatment strategies for hypertrophic scars and keloids, Int. J. Mol. Sci., 2018, vol. 19, no. 3.

Li, W., Wang, C., Peng, X., et al., CFTR inhibits the invasion and growth of esophageal cancer cells by inhibiting the expression of NF-κB, Cell Biol. Int., 2018, vol. 42, no. 12, pp. 16801687.

Liu, X., Chen, Y., You, B., et al., Molecular mechanism mediating enteric bacterial translocation after severe burn: the role of cystic fibrosis transmembrane conductance regulator, Burns Trauma, 2021, vol. 9, art. ID tkaa042.

Livak, K. and Schmittgen, T., Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT

method, Methods, 2001, vol. 25, no. 4, pp. 402408.

Orman, M., Nguyen, T., Ierapetritou, M., et al., Comparison of the cytokine and chemokine dynamics of the early inflammatory response in models of burn injury and infection, Cytokine, 2011, vol. 55, no. 3, pp. 362371.

Sarrazy, V., Billet, F., Micallef, L., et al., Mechanisms of pathological scarring: Role of myofibroblasts and current developments, Wound Repair Regener., 2011, vol. 19, no. s1, pp. 1015.

Stacey, A., DMello, N., Graeme, J., et al., Signaling pathways in melanogenesis, Int. J. Mol. Sci., 2016, vol. 17, no. 7, art. ID 1144.

Yang, A., Sun, Y., Mao, C., et al., Folate protects hepatocytes of hyperhomocysteinemia mice from apoptosis via cystic fibrosis transmembrane conductance regulator (CFTR)-activated endoplasmic reticulum stress, J. Cell. Biochem., 2017, vol. 118, no. 9, pp. 29212932.

Zhou, Y., Zhao, Y., Du, H., et al., Downregulation of CFTR is involved in the formation of hypertrophic scars, BioMed. Res. Int., 2020, vol. 2020, art. ID 9526289.

Copyright© ICBGE 2002-2023 Coded & Designed by Volodymyr Duplij Modified 29.05.23