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Detection of the infection of the Caberne Sauvignon variety of clone origin by grape viruses
SUMMARY. The high-yielding Cabernet Sauvignon grape variety is susceptible to viral diseases, which may affect its productivity and taste quality. Our aim was to identify a Cabernet Sauvignon variety of clonal origin from the south of Ukraine; to detect the contamination of plants of this variety with harmful viruses included in the planting material certification system; to identify the causative agents of viral diseases using molecular methods and to establish the nucleotide sequence of the 2CCP coat protein gene of the detected viruses. Plants with the possible symptoms of Grapevine Fanleaf Virus (GFLV) and Grapevine Leaf Roll-Associated Virus (GLRaV) viruses were found out during the phytosanitary survey of Cabernet Sauvignon grapevine plantations. But only GFLV presence was confirmed using a real-time reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of the 2CCP coat protein gene (the GenBank accession number MN072356.1). It was established that the nucleotide sequence of the Cabernet Sauvignon plant isolate is very close to samples from regions geographically distant from Ukraine, primarily the USA, Iran and France. Identity of the Cabernet Sauvignon variety of clone origin was also proved by the microsatellite analysis.
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