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The effect of implanting fibrin matrix, associated with neonatal brain cells, on the course of experimental spinal cord injury

Medvediev V.V., Oleksenko N.P., Pichkur L.D., Verbovska S.A., Savosko S.I., Draguntsova N.G., Lontkovskiy Yu.A., Vaslovych V.V., Tsymbalyuk V.I.

 




SUMMARY. The efficacy of immediate implantation of fibrin matrix, associated with allogeneic neonatal brain neural cells of rats was investigated in a spinal cord injury (SCI) model. The animals used were white adult outbred rats (~260 g, 45 months old). The SCI model was a left-sided hemisection at the level of ~T13L1 segments. The rehabilitation treatment consisted in the immediate transplantation of the human fibrin matrix, associated with rat neonatal brain cells (NBC, n = 9), into the injury area. The reference groups had isolated SCI (trauma, Tr, n = 7) and implantation of the human fibrin matrix only (Fb, n = 6) into the injury area. The assessment of motor activity of the paretic limb was conducted using the BBB scale, the assessment of spasticity the Ashworth scale; the histological examination silver impregnation staining of the spinal cord longitudinal sections obtained in the remote period of injury. The fibrin matrix promotes the activity, growth, and differentiation of the incorporated NBC. Starting from the 2nd to the 3rd week after implantation, the level of the motor function in groups Fb and NBC corresponded to ~11 points of BBB, in Tr group ~6 points of BBB. No significant differences in this indicator between the NBC and Fb groups and between the Fb and Tr groups were recorded throughout the experiment; significant differences between the NBC and Tr groups were detected at 2 weeks, 1, 2, 3, and 5 months after injury modeling. A significant advantage of the spasticity level in the Tr group over the NBC and Fb groups was found at 6 and 7 weeks after the injury, respectively. Immediate implantation of the fibrin matrix in complex with NBC has a significant positive effect on the restoration of the motor function after laceration SCI.

Key words: neurotransplantation, fibrin matrix, spinal cord injury, neonatal brain cells, locomotor function restoration, spasticity

Tsitologiya i Genetika 2022, vol. 56, no. 2, pp. 21-37

  1. Bogomolets National Medical University, 13,Tarasa Shevchenka Blv, Kyiv, 01601, Ukraine
  2. SI Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine 32, Platona Mayborody Str., Kyiv, 04050, Ukraine
  3. Kamyanets-Podilsky city state hospital, 31, Pushkinska St., Kamianets-Podilskyi, Khmelnytskyi Oblast, 32301, Ukrain
  4. National Academy of Medical Sciences of Ukraine, 12, Gercena St., Kyiv, 04050, Ukraine

E-mail: vavo2010 gmail.com, n.oleksenko gmail.com, l.pichkur neuro.kiev.ua, verbovskayasvetlana ukr.net, savosko_s ukr.net, natalya.exlab gmail.com, yulont ukr.net, greycat312 gmail.com, tsymb777 gmail.com

Medvediev V.V., Oleksenko N.P., Pichkur L.D., Verbovska S.A., Savosko S.I., Draguntsova N.G., Lontkovskiy Yu.A., Vaslovych V.V., Tsymbalyuk V.I. The effect of implanting fibrin matrix, associated with neonatal brain cells, on the course of experimental spinal cord injury, Tsitol Genet., 2022, vol. 56, no. 2, pp. 21-37.

In "Cytology and Genetics":
V. V. Medvediev, N. P. Oleksenko, L. D. Pichkur, S. A. Verbovska, S. I. Savosko, N. G. Draguntsova, Yu. A. Lontkovskiy, V. V. Vaslovych & V. I. Tsymbalyuk Effect of Implantation of a Fibrin Matrix Associated with Neonatal Brain Cells on the Course of an Experimental Spinal Cord Injury, Cytol Genet., 2022, vol. 56, no. 2, pp. 125138
DOI: 10.3103/S0095452722020086


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