ISSN 0564-3783  

Main page
Information to authors
Editorial board
Mobile version

In Ukrainian

Export citations

Features of the nucleus and nucleoli in seedlings from mother Stankevich pine trees (Pinus brutia Var. Stankewiczii Sukacz.). with different heterozygosity levels

Korshikov I.I., Bilonozhko Yu.O., Milchevskaya Ya.G.


SUMMARY. The results of the analysis of the nucleus and nucleoli features in the seeds of Pinus brutia var. stankewiczii Sukacz under conditions of the marginal part of the distribution and recreational load area are presented. The polymorphism in the number of nucleoli and their size in the cells of seedlings from the south-eastern coast of the Crimea was found between P. brutia var. stankewiczii populations. An increase in the average area of nucleoli in the nucleus and a decrease in the nucleus-nucleolus ratio were observed for the population under the recreational pressure, which indicates higher functional activity of the genetic apparatus of cells in response to adverse conditions. The change of nucleus-nucleolus parameters depending on the level of heterozygosity of mother trees was shown. The plants with low heterozygosity tended to have larger nucleoli. The identified trends indicate the complex nature of changes in the cells of the seed progeny of P. brutia var. stankewiczii.

Key words: nucleus, nucleolus, level of heterozygosity, Pinus brutia var. stankewiczii Sukacz.

Tsitologiya i Genetika 2022, vol. 56, no. 2, pp. 14-20

  1. Donetsk Botanical Garden of the National Academy of Sciences of Ukraine, 50, Marshaka Str., Kryvyi Rih 50089 Ukraine
  2. Kryvyi Rih Botanical Garden of the National Academy of Sciences of Ukraine, 50, Marshaka Str., Kryvyi Rih 50089 Ukraine
  3. Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, 2a, Osypovskoho Str., Kyiv 04123 Ukraine

E-mail: tkacheva_ua

Korshikov I.I., Bilonozhko Yu.O., Milchevskaya Ya.G. Features of the nucleus and nucleoli in seedlings from mother Stankevich pine trees (Pinus brutia Var. Stankewiczii Sukacz.). with different heterozygosity levels, Tsitol Genet., 2022, vol. 56, no. 2, pp. 14-20.

In "Cytology and Genetics":
I. I. Korshikov, Yu. O. Bilonozhko & Ya. G. Milchevskaya Peculiarities of NucleusNucleolus Indicators of Seed Progeny in Mother Stankevich Pine trees (Pinus brutia var. stankewiczii Sukacz.) Different in Levels of Heterozygosity, Cytol Genet., 2022, vol. 56, no. 2, pp. 118124
DOI: 10.3103/S0095452722020074


Altukhov, Y.P. and Moskaleichik, F.F., Allozyme heterozygosity, metabolic rate, sexual maturation rate, and longevity, Dokl. Biol. Sci., 2006, vol. 410, pp. 416420.

Andersen, J.S., Lam, Y.W., Leung, A.K., et al., Nucleolar proteome dynamics, Nature, 2005, vol. 433, pp. 7783.

Boulon, S., Westman, B.J., Hutten, S., et al., The nucleolus under stress, Mol. Cell, 2010, vol. 40, no. 2, pp. 216227.

Britton-Davidian, J., Cazaux, B., and Catalan, J., Chromosomal dynamics of nucleolar organizer regions (NORs) in the house mouse: micro-evolutionary insights, Heredity, 2012, vol. 108, pp. 6874.

Cantwell, H. and Nurse, P., Unravelling nuclear size control, Curr. Genet., 2019, vol. 65, pp. 12811285.

Chelomina, G.N., Rozhkovan, K.V., Burundukova, O.L., et al., Age-dependent and tissue-specific alterations in the rDNA clusters of the Panax ginseng C. A. Meyer cultivated cell lines, Biomolecules, 2020, vol. 10, no. 10, art. ID 1410.

Derenzini, M., Pasquinelli, G., ODonohue, M.-F., et al., Structural and functional organization of ribosomal genes within the mammalian cell nucleolus, J. Histochem. Cytochem., 2006, vol. 54, no. 2, pp. 131145.

De Storme, N. and Mason, A., Plant speciation through chromosome instability and ploidy change: Cellular mechanisms, molecular factors and evolutionary relevance, Curr. Plant Biol., 2014, vol. 1, pp. 1033.

Hein, N., Sanij, E., and Quin, J., et al., The nucleolus and ribosomal genes in aging and senescence, in Senescence, London: IntechOpen, 2012, pp. 171208.


Khrolenko, Yu.A., Burundukova, O.L., Lauve, L.S., et al., Characterization of the variability of nucleoli in the cells of Panax ginseng Meyer in vivo and in vitro, J. Ginseng Res., 2012, vol. 36, no. 3, vol. 322326.

Korshikov, I.I. and Gorlova, E.M., Genetic structure, subdivision, and differentiation in stankewiczii pine (Pinus stankewiczii (Sukacz.) Fomin) populations from mountainous Crimea, Russ. J. Genet., 2006, vol. 42, no. 6, pp. 672680.

Korshikov, I.I., Tkacheva, Yu.A., and Privalikhin, S.N., Cytogenetic abnormalities in Norway spruce (Picea abies (L.) Karst.) seedlings from natural populations and an introduction plantation, Cytol. Genet., 2012, vol. 46, pp. 280284.

Korshikov, I.I., Lapteva, Ye.V., and Tkachova, Yu.A., Variation in quantitative-dimensional characteristics of nucleoli and nuclei in seed cells of Pinus pallasiana D. Don (protected and human-disturbed areas in the steppe zone of Ukraine), Ukr. Bot. J., 2013, vol. 70, no. 6, pp. 805812.

Korshikov, I.I., Milchevskaya, Ya.G., Tkacheva, Yu.A., et al., Nuclear-nucleolar polymorphism in the regional populations off our species of conifers Factors, Exp. Evol. Org., 2013, vol. 12, pp. 5054.

Korshikov, I.I., Tkachova, Yu.A., Lapteva, H.V, et al., The nucleus-nucleolus variation in seed progeny of Pinus sylvestris L. var. cretacea Kalenicz. ex Kom. among seed yield of different years from Melovaya flora natural reserve, Factors Exp. Evol. Org., 2014, vol. 15, pp. 196200.

Korshikov, I.I., Kalafat, L.A., and Milchevskaya, Y.G., Genetic diversity and mating system of Pinus brutia var. Stankewiczii sukacz. in small localities of Sudak (Crimea), Cytol. Genet., 2015, vol. 49, no. 2, pp. 2937.

Krasikova, A. and Kulikova, T., Identification of genomic loci responsible for the formation of nuclear domains using lampbrush chromosomes, Noncoding RNA., 2019, vol. 6, no. 1, art. ID 1.

Kumar, P. and Singhal, V.K., Nucleoli migration coupled with cytomixis, Biologia, 2016, vol. 71, pp. 651659.

Lafontaine, D.L.J., Riback, J.A., Bascetin, R., et al., The nucleolus as a multiphase liquid condensate, Nat. Rev. Mol. Cell Biol., 2021, vol. 22, pp. 165182. 80-020-0272-6

Ma, T.-H., Lee, L.-W., Lee, Ch.-Ch., et al., Genetic control of nucleolar size: An evolutionary perspective, Nucleus, 2016, vol. 7, no. 2, pp. 112120.

Ma, T.-H., Chen, P.-H., Chin-Ming Tan, B., et al., Size scaling of nucleolus in Caenorhabditis elegans embryos, Biomed. J., 2018, vol. 41, no. 5, pp. 333336.

Manzano, A.I., Herranz, R., Manzano, A., et al., Early effects of altered gravity environments on plant cell growth and cell proliferation: characterization of morphofunctional nucleolar types in an Arabidopsis cell culture system, Front. Astron. Space Sci., 2016, vol. 3, art. ID 2.

Mayer, C. and Grummt, I., Cellular stress and nucleolar function, Cell Cycle, 2005, vol. 4, no. 8, pp. 10361038.

Montiel, E.E., Manrique-Poyato, M.I., Rocha-Sánchez, S.M., et al., Nucleolus size varies with sex, ploidy and gene dosage in insects, Physiol. Entomol., 2012, vol. 37, pp. 145152.

Olson, M.O. and Dundr, M., Nucleolus: Structure and Function, Chichester: Wiley, 2015.


Severine, B., Westman, B.J., Saskia, H., et al., The nucleolus under stress, Mol. Cell, 2010, vol. 40, no. 2, pp. 216227.

Sobol, M.A., Role of the nucleolus in plant cell response to environmental physical factors, Cytol. Genet., 2001, vol. 35, no. 3, pp. 7284.

Tikhonova, I.V., Correlations of heterozygosity with sexual type and sensitivity of Pinus sylvestris L. trees to the influence of environmental factors, Contemp. Probl. Ecol., 2015, vol. 8, pp. 457463.

Tkachova, Yu.O. and Korshikov, I.I., Nuclear-nuclear polymorphism of the seed progeny Picea abies (L.) Karst. (Pinaceae) in natural populations and introductory stands, Ukr. Bot. J., 2012, vol. 69, no. 6, pp. 919925.

Velichko, A.K., Razin, S.V., Kantidze, O.L., DNA damage response in nucleoli, Mol. Biol., 2021, vol. 55, pp. 182192.

Voytyuk, V. and Andreeva, V., Nucleolus activity in sprout meristem of scotch pine plus trees, Bull. Ukr. Assoc. Genet. Breed., 2009, vol. 7, no. 2, pp. 177183.

Yang, K., Yang, J., and Yi, J., Nucleolar stress: hallmarks, sensing mechanism and diseases, Cell Stress, 2018, vol. 2, no. 6, pp. 125140.

Zharskaia, O.O. and Zatsepina, O.V., Dynamics and mechanisms of the nucleolus reorganization during mitosis, Tsitologiia, 2007, vol. 49, no. 5, pp. 355369.

Copyright© ICBGE 2002-2023 Coded & Designed by Volodymyr Duplij Modified 31.01.23