ISSN 0564-3783  

Main page
Information to authors
Editorial board
Mobile version

In Ukrainian

Export citations

Genetic polymorphism of the BoLA-DRB3.2 locus of ukrainian cattle breeds

Suprovych ., Salyha Y., Suprovych ., Fedorovych Ye., Fedorovych V., Chornyj .


SUMMARY. The results of the study of exon 2 polymorphism of the BoLA-DRB3 gene in four domestic cattle breeds: Ukrainian black-and-white dairy (UBWM, n = 293), Ukrainian red-and-white dairy (URWM, n = 131), Gray Ukrainian (n = 93) and Ukrainian white-headed (UWH, n = 49) are presented. Allele typing was performed by the PLR-PDRF method. Genetic profiles of the breeds were formed according to the following indices: allele (Pa) and genotype (Ga) frequencies, observed (Ho) and expected (He) heterozygosity, Wright fixation index (FIS), total (Na) and effective number (Ae) of alleles, indices Shannon (I) and Pielu (J). The maximum number of alleles was in the UBWM breed (Na = 37). For the URWM, UWH, and Gray breeds, 31, 29, and 28 va-riants were identified, respectively. Two breeds were ty-ped with no established nomenclature alleles: Gray *jba, *jab, *jbb, *nad and *nda, UWH *nab, *mdb, *iab, *gbb, *fbd, *naa, whose proportion was 8.9 and 7.1 %, respectively. The number of informative alleles was: UBWM 55.8 % (7 alleles), URWM 60.4 % (6), Gray 69.9 % (4), and UWH 65.3 % (7). The expected heterozygosity and Wright index had the following values: UBWM He = 0.942; FIS = 0.022; URWM He = 0.93; FIS = 0.032; Gray He = 0.774; FIS = 0.041; WHU He = 0.927; FIS = 0.035. The following biodiversity indices were obtained for the studied rocks: UBWM Ae = 17.2; I = 3.13; J = 0.6; WHU Ae = 13.7; I = 2.93; J = 0.61 and URWM Ae = 11.5; I = 2.87; J = 0.558, and Gray Ae = 4.42; I = 2.21; J = 0.455. Analysis of dendrograms based on genetic distances showed that domestic breeds and Holstein are combined in the same cluster, indicating their genetic similarity behind the BoLA-DRB3 gene. Exon 2 polymorphism data of BoLA-DRB3 gene showed that the greatest genetic diversity among Ukrainian cattle is characteristic of UBWM breed. The results obtained contribute to better understanding of genetic diversity and distribution of BoLA-DRB3 gene alleles in cattle in Ukraine and increase our knowledge about global variability of BoLA-DRB3 gene, which forms immune response to protect against pathogens.

Key words: polymorphism, cattle, biodiversity, BoLA-DRB3 gene, alleles

Tsitologiya i Genetika 2022, vol. 56, no. 4, pp. 10-23

  1. Higher educational institution Podillia state university, 13, Shevchenko Str., Kamianets-Podilskyi, 32316, Ukraine
  2. Institute of Animal Biology NAAS, V.Stus Street 38,Lviv 79034,Ukraine

E-mail: yursalyha

Suprovych ., Salyha Y., Suprovych ., Fedorovych Ye., Fedorovych V., Chornyj . Genetic polymorphism of the BoLA-DRB3.2 locus of ukrainian cattle breeds, Tsitol Genet., 2022, vol. 56, no. 4, pp. 10-23.

In "Cytology and Genetics":
T. M. Suprovych, Yu. T. Salyha, M. P. Suprovych, E. I. Fedorovych, V. V. Fedorovych & I. O. Chornyj Genetic Polymorphism of BoLA-DRB3.2 Locus in Ukrainian Cattle Breeds, Cytol Genet., 2022, vol. 56, no. 4, pp. 319330
DOI: 10.3103/S0095452722040089


Bai, L., Otsuki, H., Sato, H., Kohara, J., Isogai, E., Takeshima, S.N., and Aida, Y., Identification and characterization of common B cell epitope in bovine leukemia virus via high-throughput peptide screening system in infected cattle, Retrovirology, 2015, vol. 12, no. 106, pp. 114.

Begon, M., Townsend, C.R., and Harper, J.L., Ecology: From Individuals to Ecosystems, Oxford: Blackwell, 2006.

Behl, J.D., Verma, N.K., Behl, R., and Sodhi, M., Genetic variation of the major histocompatibility complex DRB3.2 locus in the native Bos indicus cattle breeds. Asian-Aust. J. Anim. Sci., 2009, vol. 22, no. 11, pp. 14871494.

Behl, J.D., Verma, N.K., Tyagi, N., Mishra, P., Behl, R., and Joshi, B.K., The major histocompatibility complex in bovines: a review, ISRN Vet, Sci., 2012, vol. 2012, art. ID 872710.

Bolaños, I., Hernandez, D., and Alvarez, L., Asociación de los alelos del gen BoLA-DRB3 con la infección natural de Babesia spp en el Ganado criollo Hartón del Valle, Arch. Zootec., 2017, vol. 66, no. 253, pp. 113120.

Chakraborty, D., Singh, A., Tantia, M., Verma, A., and Chakravarty, A., Genetic polymorphism of BoLA-DRB3.2 locus in Sahiwal cattle, Anim. Sci. Rep., 2015, vol. 9, no. 1, pp. 3340.

Dietz, A.B., Cohen, N.D., Timms, L., and Kehrli, M.E., Bovine lymphocyte antigen class II alleles as risk factors for high somatic cell counts in milk of lactating dairy cows, J. Dairy Sci., 1997, vol. 80, no. 2, pp. 406412.

Duangjinda, M., Jindatajak, Y., Tipvong, W., Sriwarothai, J., Pattarajinda, V., Katawatin, S., and Boonkum, W., Association of BoLA-DRB3 alleles with tick-borne disease tolerance in dairy cattle in a tropical environment, Vet. Parasitol., 2013, vol. 196, nos. 34, pp. 314320.

Fernández, I.G., Leyva-Baca, I., Rodríguez-Almeida, F., Ulloa-Arvizu, R., Ríos-Ramírez, J.G., Gayosso-Vázquez, A., and Alonso-Morales, R.A., Creole cattle from northwestern Mexico has high genetic diversity in the locus DRB3.2, Anim. Genet. Res., 2015.

Firouzamandi, M., Shoja, J., Bazegari, A., and Roshani, E., Study on the association of BoLA-DRB3.2 alleles with clinical mastitis in Iranian Holstein and Sarabi (Iranian native) cattle, Afr. J. Biotech., 2010, vol. 9, no. 15, pp. 22242228.

Gelhaus, A., Schnittger, L., Mehlitz, D., Horstmann, R.D., and Meyer, C.G., Sequence and PCR-RFLP analysis of 14 novel BoLA-DRB3 alleles, Anim. Genet., 1995, vol. 26, no. 3, pp. 147153.

Gilliespie, B.E., Jayarao, B.M., Dowlen, H.H., and Oliver, S.P., Analysis and frequency of bovine lymphocyte antigen DRB3.2 alleles in Jersey cows, J. Dairy Sci., 1999, vol. 82, no. 9, pp. 20492053.

Giovambattista, G., Moe, K.K., Polat, M., Borjigin, L., Hein, S.T., Moe, H.H., Takeshima, S.N., and Aida, Y., Characterization of bovine MHC DRB3 diversity in global cattle breeds, with a focus on cattle in Myanmar, BMC Genetic, 2020a, vol. 21, art. ID 95. 1186/s12863-020-00905-8

Giovambattista, G., Takeshima, S.N., Moe, K.K., Pereira, Rico, J.A., Polat, M., Loza Vega, A., Arce Cabrera, O.N., and Aida, Y., BoLA-DRB3 genetic diversity in Highland Creole cattle from Bolivia, HLA, 2020b, vol. 96, no. 6, pp. 688696.

Gómez-Castro, S., Trujillo-Bravo, E., Carlos-Vicente, D., and Agron, I., BoLA-DRB3 Polymorphism in Colombian synthetic cattle Lucerna and association with somatic cell counts and mastitis, Rev. Colomb. Cienc. Pec., 2006, vol. 19, no. 3, pp. 270279.

Goszczynski, D.E., Ripoli, M.V., Takeshima, S.N., Baltian, L., Aida, Y., and Giovambattista, G., Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle, Tissue Antigens, 2014, vol. 83, pp. 180183.

Gowane, G.R., Sharma, A.K., Sankar, M., Narayanan, K., Das, B., Subramaniam, S., and Pattnaik, B., Association of BoLA DRB3 alleles with variability in immune response among the crossbred cattle vaccinated for foot-and-mouth disease (FMD), Res. Vet. Sci., 2012, vol. 95, no. 1, pp. 156163.

Gowane, G.R., Vandre, R.K., Nangre, M., and Sharma, A.K., Major histocompatibility complex (MHC) of bovines: an insight into infectious disease resistance, Livest. Res. Int., 2013, vol. 1, no. 2, pp. 4657.

Groom, M.J., Meffe, G.K., and Carroll, C.R., Principles of Conservation Biology, Sinauer Associates, 2005.

Hedrick, P.W., Whittam, T.S., and Parham, P., Heterozygosity at individual amino acid sites: Extremely high levels for HLA-A and -B genes, Proc. Natl. Acad. Sci. U. S. A., 1991, vol. 88, no. 13, pp. 58975901.

Hedrick, P., Genetic of Populations, Sudbury: Mass Jones and Bartlett Publishers, 2010.

Jost, L., Partitioning diversity into independent alpha and beta components, Ecology, 2007, vol. 88, no. 10, pp. 24272439.

Juliarena, M.A., Poli, M., Sala, L., Ceriani, C., Gutierrez, S., Dolcini, G., Rodríguez, E.M., Mariño, B., Rodríguez- Dubra, C., and Esteban, E.N., Association of BLV infection profiles with alleles of the BoLA-DRB3.2 gene, Anim. Genet., 2008, vol. 39, no. 4, pp. 432438.

Juliarena, M.A., Barrios, C.N., Ceriani, M.C., and Esteban, E.N., Hot topic: Bovine leukemia virus (BLV)-infected cows with low proviral load are not a source of infection for BLV-free cattle, J. Dairy Sci., 2016, vol. 99, no. 6, pp. 45864589.

Kumari, N., Loat, S., Saini, S., Dhilor, N., Kumar, A., and Kataria, R.S., Role of BoLA-RB3 genetic diversity against resistance to mastitis in cattle: Review, J. Vet. Sci. Res., 2019, vol. 1, pp. 3036.

Lamba, H., Sharma, D., Singh, S., Tiwari, M., Goel, R., Pandey, V., and Singh, S., BoLA-DRB3 polymorphism and their association with milk production traits in Indian cattle breeds, J. Livest. Biodiversity, 2017, vol. 7, no. 1, pp. 5259.

Lazebnaya, I.V., Perchun, A.V., and Lazebny, O.E., Intrabreed and interbreed variation of the BOLA-DRB3.2 gene in the Kostroma and Yaroslavl indigenous Russian cattle breeds, Immunogenetics, 2020, vol. 72, nos. 67, pp. 355366.

Lei, W., Liang, Q., Jing, L., Wang, C., Wu, X., and He, H., BoLA-DRB3 gene polymorphism and FMD resistance or susceptibility in Wanbei cattle, Mol. Biol. Rep., 2012, vol. 39, no. 9, pp. 92039209.

Lo, C.W., Borjigin, L., Saito, S., Fukunaga, K., Saitou, E., Okazaki, K., Mizutani, T., Wada, S., Takeshima, S.N., and Aida, Y., BoLA-DRB3 Polymorphism is associated with differential susceptibility to bovine leukemia virus-induced lymphoma and proviral load, Viruses, 2020, vol. 12, no. 3, art. ID 352.

Maillard, J.C., Martinez, D., and Bensaid, A., An amino acid sequence encoded by the exon 2 of the BoLA DRB3 gene associated with a BoLA class 1 specificity constitutes a likely genetic marker of resistance to dermatophilosis in Brahman zebu cattle in Martinique (FWI), Ann. N. Y. Acad. Sci., 1996, vol. 791, pp. 185197.

Maillard, J.C., Renard, C., Chardon, P., Chantal, I., and Bensaid, A., Characterization of 18 new BoLA-DRB3 alleles, Anim. Genet., 1999, vol. 30, no. 3, pp. 200203.

Mandefro, A., Sisay, T., Edea, Z., Uzzaman, M.R., Kim, K.S., and Dadi, H., Genetic assessment of BoLA-DRB3 polymorphisms by comparing Bangladesh, Ethiopian, and Korean cattle, J. Anim. Sci. Technol., 2021, vol. 63, no. 2, pp. 248261.

Martinez, M.L., Machado, M.A., Nascimento, C.S., Silva, M.V., Teodoro, R.L., Furlong, J., Prata, M.C., Campos, A.L., Guimarães, M.F., Azevedo, A.L., Pires, M.F., and Verneque, R.S., Association of BoLA-DRB3.2 alleles with tick (Boophilus microplus) resistance in cattle, Genet. Mol. Res., 2006, vol. 5, no. 3, pp. 513524.

McShane, R.D., Gallagher, D.S., Newkirk, H., Taylor, J.F., Burzlaff, J.D., Davis, S.K., and Skow, L.C., Physical localization and order of genes in the class I region of the bovine, Anim. Genet., 2001, vol. 32, no. 5, pp. 235239.

Miyasaka, T., Takeshima, S.N., Matsumoto, Y., Kobayashi, N., Matsuhashi, T., Miyazaki, Y., Tanabe, Y., Ishibashi, K., Sentsui, H., and Aida, Y., The diversity of bovine MHC class II DRB3 and DQA1 alleles in different herds of Japanese Black and Holstein cattle in Japan, Gene, 2011, vol. 472, nos. 12, pp. 4249.

Morales, J., Herrera, A., and Zuluaga, J., Association of BoLA DRB3 gene polymorphisms with BoHV-1 infection and zootechnical traits, Open Vet. J., 2020, vol. 10, no. 3, pp. 331339.

Nassiry, M.R., Shahroodi, F.E., Mosafer, J., Mohammadi, A., Manshad, E., Ghazanfari, S., Mohammad Abadi, M.R., and Sulimova, G.E., Analysis and frequency of bovine lymphocyte antigen (BoLA-DRB3) alleles in Iranian Holstein cattle, Genetika, 2005, vol. 41, no. 6, pp. 817822.

Nei, M., Genetic distance between populations, Am. Naturalist, 1972, vol. 106, no. 949, pp. 283292.

Nei, M. and Chesser, R.K., Estimation of fixation indices and gene diversities, Ann. Hum. Genet., 1983, vol. 47, no. 3, pp. 253259.

Nei, M., Tajima, F., and Tateno, Y., Accuracy of estimated phylogenetic trees from molecular data. II. Gene frequency data, J. Mol. Evol., 1983, vol. 19, no. 2, pp. 153170.

Nikbakht Brujeni, G., Ghorbanpour, R., and Esmailnejad, A., Association of BoLA-DRB3.2 alleles with BLV infection profiles (Persistent lymphocytosis/ Lymphosarcoma) and Lymphocyte Subsets in Iranian Holstein Cattle, Biochem. Genet., 2016, vol. 54, no. 2, pp. 194207.

Pashmi, M., Qanbari, S., Ghorashi, S.A., Sharifi, A.R., and Simianer, H., Analysis of relationship between bovine lymphocyte antigen DRB3.2 alleles, somatic cell count and milk traits in Iranian Holstein population, J. Anim. Breed. Genet., 2009, vol. 126, no. 4, pp. 296303.

Paul, W.E., Fundamental Immunology, Lippincott Williams & Wilkins, 2012.

Pielou, E.C., Shannons formula as a measure of species diversity: Its use and misuse, Am. Naturalist, 1966, vol. 100, no. 914, pp. 463465.

Pokorska, J., Kułaj, D., Dusza, M., Ochrem, A., and Makulska, J., The influence of BoLA-DRB3 alleles on incidence of clinical mastitis, cystic ovary disease and milk traits in Holstein Friesian cattle, Mol. Biol. Rep., 2018, vol. 45, no. 5, pp. 917923.

Rupp, R., Hernandez, A., and Mallard, B., Association of bovine leukocyte antigen (BoLA) DRB3.2 with immune response, mastitis, and production and type traits in Canadian Holsteins, J. Dairy Sci., 2007, vol. 90, no. 2, pp. 1029 1038.

Ruzina, M.N., Shtyfurko, T.A., Mohammad Abadi, M.R., Gendzhieva, O.B., Cedev, C., and Sulimova, G.E., Polymorphism of the BoLA-DRB3 gene in the Mongolian, Kalmyk, and Yakut cattle breeds, Rus. J. Genet., 2010, vol. 46, no. 4, pp. 517525.

Salim, B., Takeshima, S.N., Nakao, R., Moustafa, M., Ahmed, M., Kambal, S., Mwacharo, J., Alkhaibari, A., and Giovambattista, G., BoLA-DRB3 gene haplotypes show divergence in native Sudanese cattle from taurine and indicine breeds nature research, Sci. Rep., 2021, vol. 11, art. ID 17202.

Saravanan, R., Murali, N., Thiruvenkadan, A.K., and Das, D.N., Comparitive genome sequence analysis of bovine lymphocyte antigen BoLA DRB3.2 alleles in Deoni and Ongole (Bos indicus) cattle breeds of India, Indian J. Anim. Res., 2021.

Sharif, S., Mallard, B.A., Wilkie, B.N., Sargeant, J.M., Scott, H.M., Dekkers, J.C., and Leslie, K.E., Associations of the bovine major histocompatibility complex DRB3 (BoLA-DRB3) alleles with occurrence of disease and milk somatic cell score in Canadian dairy cattle, Anim. Genet., 1998, vol. 29, pp. 185193.

Sherwin, W.B., Entropy, or information, unifies ecology and evolution and beyond, Entropy, 2018, vol. 20, no. 10, art. ID 727.

Sulimova, G.E., DNA markers in genetic research: marker types, their properties and applications, Success Modern Biol., 2004, vol. 124, no. 3, pp. 260271.

Sun, L., Song, Y., Riaz, H., and Yang, L., Effect of BoLA-DRB3 exon 2 polymorphisms on lameness of Chinese Holstein cows, Mol. Biol. Rep., 2013, vol. 40, no. 2, pp. 10811086.

Suprovich, T. and Mokhnachova, N., Gene polymorphism of economically-useful traits in Ukrainian gray cattle breed, Anim. Biol., 2017, vol. 19, no. 1, pp. 111118.

Suprovych, T.M., Suprovych, M.P., Koval, T.V., Karchevska, T.M., Chepurna, V.A., Chornyi, I.O., and Berezhanskyi, A.P., BoLA-DRB3 gene as a marker of susceptibility and resistance of the Ukrainian blackpied and red-pied dairy breeds to mastitis, Regul. Mech. Biosyst., 2018a, vol. 9, no. 3, pp. 363368.

Suprovych, T.M., Dyman, T.M., Suprovych, M.P., Karchevska, T.M., Koval, T.V., Kolodiy, V.A., Population genetic structure of the Ukrainian black-pied dairy breed with the genome BoLA-DRB3, Regul. Mech. Biosyst., 2018b, vol. 9, no. 4, pp. 568577.

Suprovych, T.M., Vishchur, O.I., Suprovych, M.P., and Chepurna, V.A., Relationship between alleles of gene BoLA-DRB3 and somatic cells amount in milk of Ukrainian black-and-white dairy breed, Anim. Biol., 2019, vol. 21, no. 4, pp. 7583.

Suprovych, T.M., Suprovych, M.P., Kolinchuk, R.V., Karchevska, T.M., Chornyi, I.O., and Kolodiy, V.A., Association of BoLA-DRB3.2 alleles with fusobacteriosis in cows, Regul. Mech. Biosyst., 2020, vol. 11, no. 2, pp. 249254.

Suprovych, T.M., Suprovych, M.P., Mokhnachova, N.B., Biriukova, O.D., Strojanovska, L.V., and Chepurna, V.A., Genetic variability and biodiversity of Ukrainian Gray cattle by the BoLA-DRB3 gene, Regul. Mech. Biosyst., 2021, vol. 12, no. 1, pp. 3341.

Takeshima, S., Saitou, N., Morita, M., Inoko, H., and Aida, Y., The diversity of bovine MHC class II DRB3 genes in Japanese black, Japanese shorthorn, Jersey and Holstein cattle in Japan, Gene, 2003, vol. 316, pp. 111118.

Takeshima, S.N., Miyasaka, T., Polata, M., Kikuya, M., Matsumoto, Y., Mingala, C.N., Villanueva, M.A., Salces, A.J., Onuma, M., and Aida, Y., The great diversity of major histocompatibility complex class II genes in Philippine native cattle, Meta Gene, 2014, vol. 2, pp. 176190.

Takeshima, S.N., Giovambattista, G., Okimoto, N., Matsumoto, Y., Rogberg-Muñoz, A., Acosta, T.J., Onuma, M., and Aida, Y., Characterization of bovine MHC class II DRB3 diversity in South American Holstein cattle populations, Tissue Antigens, 2015, vol. 86, no. 6, pp. 419430.

Takeshima, S., Corbi-Botto, C., Giovambattista, G., and Aida, Y., Genetic diversity of BoLA-DRB3 in South American Zebu cattle populations, BMC Genet., 2018, vol. 19, art. ID 33.

Tayeng, K., Tomar, S.S., Nanavati, S., Aich, R., Ignetious, S., and Nagoriya, S.K., BoLA-DRB3 gene polymorphism and its association with lactation yield and milk constituents in crossbred cattle, Indian J. Vet. Sci. Biotechnol., 2021, vol. 17, pp. 7175.

Valenzano, M., Caffaro, M., Lia, V., Poli, M., and Wilkowsky, S., Diversity of the BoLA-DRB3 Gene in Cattle Breeds from Tropical and Subtropical Regions of south America, 2021.

Van Eijk, M.J.T., Stewart-Haynes, J.A., and Lewin, H.A., Extensive polymorphism of the BOLA-DRB3 gene distinguished by PCR-RFLP, Anim. Genet., 1992, vol. 23, no. 6, pp. 483496.

Villalobos-Cortés, A.R. and González, R., Sequences of gene BoLA-DRB3.2 from the Guaymi and Guabala creole cattle of Panama, Revista Ciencia Agropecuaria, 2018, vol. 28, pp. 2236.

Zambrano, A.J., Echeverri, Z.J., and López-Herrera, A., Association of gene BoLA DRB3.2 with production traits in a dairy herd of Antioquia, Colombia, Rev. MVZ Córdoba, 2014, vol. 19, no. 2, pp. 41164129.

Zietkiewicz, E., Rafalski, A., and Labuda, D., Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification, Genomics, 1994, vol. 20, no. 2, pp. 176183.

Copyright© ICBGE 2002-2023 Coded & Designed by Volodymyr Duplij Modified 29.01.23