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SEF1 and VMA1 genes regulate riboflavin biosynthesis in the flavinogenic yeast Candida famata

Andreieva Y., Lyzak O., Liu Wen, Kang Yingqian, Dmytruk K., Sibirny A.


SUMMARY. Riboflavin (vitamin B2) is an important component of the diet of living organisms due to its serving as a precursor of flavin coenzymes FMN (flavin mononucleotide) and FAD (flavin adenine dinucleotide) involved in numerous enzymatic reactions. It is known that flavinogenic yeast C. famata is able of riboflavin oversynthesis under condition of iron starvation, but the regulation of this process remains unknown. It was shown that the deletion of SEF1 gene (encoding transcription activator) blocked the ability of riboflavin oversynthesis under iron-limiting conditions. It was determined that SEF1 promoters of other flavinogenic yeasts (Candida albicans and Candida tropicalis), fused with SEF1 ORF of C. famata can restore the oversynthesis of riboflavin in sef1Δ mutant. The disruption of VMA1 gene (coding for vacuolar ATPase subunit A) led to oversynthesis of riboflavin in C. famata in iron complete medium.

Key words: riboflavin, Candida famata, VMA1, SEF1, yeast

Tsitologiya i Genetika 2020, vol. 54, no. 5, pp. 3-11

  1. Institute of Cell Biology National Academy of Sciences of Ukraine, Drahomanov St, 14/16, Lviv, 79005, Ukraine
  2. Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
  3. Guizhou Medical University, Guiyang, Guizhou 550025 China
  4. University of Rzeszow, Zelwerowicza 4, 35-601 Rzeszow, Poland

E-mail: sibirny

Andreieva Y., Lyzak O., Liu Wen, Kang Yingqian, Dmytruk K., Sibirny A. SEF1 and VMA1 genes regulate riboflavin biosynthesis in the flavinogenic yeast Candida famata, Tsitol Genet., 2020, vol. 54, no. 5, pp. 3-11.

In "Cytology and Genetics":
Y. Andreieva, O. Lyzak, Wen Liu, Yingqian Kang, K. Dmytruk & A. Sibirny SEF1 and VMA1 Genes Regulate Riboflavin Biosynthesis in the Flavinogenic Yeast Candida Famata , Cytol Genet., 2020, vol. 54, no. 5, pp. 379385
DOI: 10.3103/S0095452720050023


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