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Evaluation of phytotoxicity and mutagenicity of novel DMAEMA-containing gene carriers

Finiuk N., Romanyuk N., Mitina N., Lobachevska O., Zaichenko A., Terek O., Stoika R.


SUMMARY. A use of novel carriers for gene delivery is rapidly gro-wing, thus, investigation of potential phytotoxic and mutagenic action of gene delivery carriers is important for excluding their negative side effects. We found that poly-DMAEMA carriers used in 0,0025 % dose exhibited weak cytotoxic effect towards Allium cepa plant. In higher dose (0,025 %), they slightly (by 26Ц55 %) increased the level of catalase activity, but they did not affect the level of superoxide dismutase activity and malonic dialdehyde content in roots of A. cepa. The results of ana-telophase test in A. cepa did not demonstrate a genotoxic activity of the polymeric carriers used in 0.0025 % concentration and its higher dose (0,025 %). Slight genotoxic activity was detected only for BGP24 and BGP26, PEG-containing poly-DMAEMA carriers used in 0,025 %. The DMAEMA-based polymers did not possess muta-genic potential estimated in Ames test (ЦS9 and +S9). Thus, low phytotoxicity and absence of mutagenic action of novel polymeric carriers suggest their potential as promising nanocarriers for gene delivery into plant cells.

Key words: poly(2-dimethylamino)ethyl methacrylate, polymeric carrier, ana-telophase assay, Ames test, catalase, superoxide dismutase, malonic dialdehyde

Tsitologiya i Genetika 2020, vol. 54, no. 5, pp. 75-88

  1. Institute of Cell Biology of National Academy of Sciences of Ukraine, Drahomanov st., 14/16, Lviv, 79005, Ukraine
  2. Ivan Franko National University of Lviv, Hrushevskyy st., 4, Lviv, 79005, Ukraine
  3. Lviv Polytechnic National University, Bandera st., 12, Lviv, 79013, Ukraine
  4. Institute of Ecology of the Carpathians of National Academy of Sciences of Ukraine, Kozelnytska st., 4, Lviv, 79026, Ukraine

E-mail: stoika.rostyslav

Finiuk N., Romanyuk N., Mitina N., Lobachevska O., Zaichenko A., Terek O., Stoika R. Evaluation of phytotoxicity and mutagenicity of novel DMAEMA-containing gene carriers, Tsitol Genet., 2020, vol. 54, no. 5, pp. 75-88.

In "Cytology and Genetics":
N. Finiuk, N. Romanyuk, N. Mitina, O. Lobachevska, A. Zaichenko, O. Terek & R. Stoika Evaluation of Phytotoxicity and Mutagenicity of Novel DMAEMA-Containing Gene Carriers, Cytol Genet., 2020, vol. 54, no. 5, pp. 437Ц448
DOI: 10.3103/S0095452720050096


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