Change of morphostructural characteristics and metabolic profile of Walker-256 carcinosarcoma under the impact of exogenous lactoferrin

Naleskina L.A., Lukianova N.Yu., Lozovska Yu.V., Todor I.M., Andrusishyna I.M., Kunska L.M., Chekhun V.F.

SUMMARY. Numerous current investigations in the field of experimental oncology have demonstrated that the results of fundamental studies, conducted on current methodological level, may serve as a considerable foundation for their application in the clinical practice. To determine the inhibiting effect of exogenous lactoferrin (LF) in the doses of 1 and 10 mg/kg of the bodyweight of animals in the system in vivo, using Walker-256 carcinosarcoma, the safety of its application, the specificities of changes in cytoarchitectonics of the tumour tissue, the disturbance in the indices of energy exchange and essential homeostasis on the level of the tumour and organism, as well as associative connections and mechanisms, conditioning the abovementioned transformations. Outbred rats with grafted Walker-256 carcinosarcoma, which were abdominally administered exogenous LF nine times in the doses of 1 and 10 mg/kg of the bodyweight of animals, and the animals, which served as a control, were used. The morphological method of estimating the changes in cytoarchitectonics of tumours under the impact of LF was applied. The safety of applying exogenous LF was determined using the cytogenetic method of Hayashi. The content of essential elements was determined using the method of atomic-emission spectroscopy, the content of energy exchange indices was defined using the atomic biochemistry and immunoenzymatic analyzer. It was determined that exogenous LF in the investigated doses leads to inhibiting the growth of Walker-256 carcinosarcoma, which is manifested in unidirectional changes in its architectonics: the segregation of tumour cells, pycnosis, hyperchromatosis of nuclei, necrobiosis phenomena, necrosis and, as a result of the decrease in the total number of tumour cells, – dilatation of vessels, thinning of walls, hemorrhages. There are changes in the bioenergetic phenotype of tumour cells: the decrease in the content of glucose and lactate. The impact of LF leads to the impairment of the homeostasis of essential elements, including calcium, iron, zinc in the tumour tissue and blood plasma. The mechanisms, which may condition the abovementioned effect of LF, were determined based on the conducted studies of impairments in tumour cytoarchitectonics, including the vessels, under the effect of exogenous LF.

Key words: Walker-256 carcinosarcoma, exogenous lactoferrin, tumour cytoarchitectonics, energy exchange, glucose, lactate, essential elements

Tsitologiya i Genetika 2020, vol. 54, no. 3, pp. 54-68

1. R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
2. SE Yu.I. Kundiev Institute of Labour Medicine, NAMS, Kyiv 01033, Ukraine

E-mail: Naleskina ukr.net, nataluk10 gmail.com, lozovskaya.2012 ukr.net, andrusyshyna.in gmail.com, luba110 ukr.net, chekhun onconet.kiev.ua

Naleskina L.A., Lukianova N.Yu., Lozovska Yu.V., Todor I.M., Andrusishyna I.M., Kunska L.M., Chekhun V.F. Change of morphostructural characteristics and metabolic profile of Walker-256 carcinosarcoma under the impact of exogenous lactoferrin, Tsitol Genet., 2020, vol. 54, no. 3, pp. 54-68.

In "Cytology and Genetics":
L. A. Naleskina, N. Yu. Lukianova, Yu. V. Lozovska, I. M. Todor, I. M. Andrusishyna, L. M. Kunska & V. F. Chekhun Changes of Morphological Characteristics and Metabolic Profile of Walker-256 Carcinosarcoma under the Impact of Exogenous Lactoferrin, Cytol Genet., 2020, vol. 54, no. 3, pp. 220–232
DOI: 10.3103/S0095452720030093

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