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The linkage of polymorphic variants of genes GH, PRL, PIT-1 and milk productivity of cows with morphology of cumulus-oocyte complex, sampled post mortem

Pozovnikova M.V., Rotar L.N., Kudinov A.A., Dementieva N.V.


SUMMARY. In recent years the aim of dairy cattle breeding was to increase milk production traits. At the same time, there is observed decrease of fertility traits of cows, and as a result, early culling of animals. This fact may be explained by an almost impossibility to determine the reasons of aggravated reproduction, and the difficulty of estimating them. One of the causes of a decrease in the fertility of cows is the decline in the quality of oocytes, capable of fertilization. Therefore, the study of the ratio between the number and quality of oocytes, capable of fertilization, and the level of milk productivity of cows, as well as the connection with polymorphic variants of the GH, PRL, and Pit-1 genes, is of scientific interest. The aim of our work was to determine the dependence of the quality and quantity of postmortem cow oocytes on the level of milk productivity and genetic profile for the genes GH, PRL and Pit-1. There was a high incidence of allele L of the GH gene 0,942, allele A of the PRL gene 0,889 and allele B of the Pit-1 gene 0,710. The data obtained showed that a high yield of COC on average per ovary was obtained in a group of animals with the LL genotype of the GH gene (LL to LV +7,54 pcs, p ≤ 0,05), along with a significant amount of viable (LL to LV +4,5 pcs, p ≤ 0,05). In individuals with the AA genotype of the PRL gene, the number of isolated viable COCs on average per ovary was higher compared to those with the AB genotype (+4,84 pcs, p ≤ 0,05). An analysis of the association of polymorphic variants of the Pit-1 gene with the quantity and quality of COC did not reveal significant differences in the studied group of individuals. The assessment of the relationship of polymorphic variants of the studied genes with the indicators of breeding value (BV) yield, fat and protein showed that only animals with the LV genotype exceeded their peers with the LL genotype of the GH gene in milk fat and protein output by 8,3 kg (p ≤ 0,05) and 6,0 kg (p ≤ 0,01), respectively. For the remaining SNP of the analyzed genes, no significant differences were obtained.

Key words: Dairy cattle, milk yield, GH gene, PRL gene, Pit-1 gene, cumulus-oocyte complex, oocytes morphology

Tsitologiya i Genetika 2020, vol. 54, no. 3, pp. 44-53

  1. Russian Research Institute for Farm Animal Genetics and Breeding Branch of the L.K.Ernst Federal Science Center for Animal Husbandry (RRIFAGB),55, Moskovskoe sh., pos. Tyarlevo, St. PetersburgPushkin, 196625 Russia
  2. Saint-Petersburg State Agrarian University, 2, Peterburgskoe sh., St. PetersburgPushkin, 196601 Russi

E-mail: pozovnikova, valevskaya bk, kudinov_aa, dementevan

Pozovnikova M.V., Rotar L.N., Kudinov A.A., Dementieva N.V. The linkage of polymorphic variants of genes GH, PRL, PIT-1 and milk productivity of cows with morphology of cumulus-oocyte complex, sampled post mortem, Tsitol Genet., 2020, vol. 54, no. 3, pp. 44-53.

In "Cytology and Genetics":
M. V. Pozovnikova, L. N. Rotar, A. A. Kudinov & N. V. Dementieva The Linkage of Polymorphic Variants of Genes Gh, Prl, and Pit-1 and Milk Productivity of Cows with Morphology of Cumulus-Oocyte Complex Sampled Post Mortem, Cytol Genet., 2020, vol. 54, no. 3, pp. 212219
DOI: 10.3103/S0095452720030111


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