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Molecular organization of 5S ribosomal DNA and taxonomic status of Avenella flexuosa (L.) Drejer (Poaceae)

Ishchenko I.I., Melnyk V.., Parnikoza .Y., Budzhak V.V., Panchuk .., Kunakh V.., Volkov R..

 




SUMMARY. The wavy hair-grass, Avenella flexuosa (L.) Drejer (also known as Deschampsia flexuosa (L.) Trin. or Lerchenfeldia flexuosa (L.) Schur), is a species of perennial herbaceous plants of the Poaceae family that is widespread in Eurasia, Africa, North and South America. Whether this species belongs to the Deschampsia genus has long been an open question. Taking into account that the 5S rDNA comparison is a convenient tool in the molecular taxonomy of plants, we cloned and sequenced this region of the A. flexuosa genome and evaluated the genetic distances between this species and other species of the Poeae tribe. It has been found that at least two structural classes of 5S rDNA repeats are present in the A. flexuosa genome. The spacer regions in the 5S rDNA of A. flexuosa and D. antarctica have a low level of similarity that does not exceed the level of similarity with representatives of several other genera of the Poeae tribe. The 5S rDNA clones of A. flexuosa and D. antarctica form two distinct clades on the phyllodendogram. Taking together, our results support the idea that A. flexuosa belongs to a separate genus Avenella.

Key words: molecular evolution and taxonomy, intergenic spacer of 5S rDNA, Avenella, Deschampsia, Poeae

Tsitologiya i Genetika 2020, vol. 54, no. 6, pp. 3-13

  • Yuriy Fedkovych Chernivtsi National University Ministry of Education and Science of Ukraine, Kotsiubynski str. 2, 58012 Chernivtsi, Ukraine
  • Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Zabolotnogo str., 150, 03143 Kyiv, Ukraine
  • State Institution National Antarctic Scientific Centre, Ministry of Education and Science of Ukraine, Boulevard of Taras Shevchenko 16, 01601 Kyiv, Ukraine

E-mail: r.volkov chnu.edu.ua

Ishchenko I.I., Melnyk V.., Parnikoza .Y., Budzhak V.V., Panchuk .., Kunakh V.., Volkov R.. Molecular organization of 5S ribosomal DNA and taxonomic status of Avenella flexuosa (L.) Drejer (Poaceae), Tsitol Genet., 2020, vol. 54, no. 6, pp. 3-13.

In "Cytology and Genetics":
O. O. Ishchenko, V. M. Melnyk, . Y. Parnikoza, V. V. Budzhak, . . Panchuk, V. A. Kunakh & R. A. Volkov Molecular Organization of 5S Ribosomal DNA and Taxonomic Status of Avenella flexuosa (L.) Drejer (Poaceae), Cytol Genet., 2020, vol. 54, no. 6, pp. 505513
DOI: 10.3103/S0095452720060055


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