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Polyclone structure of settlements of unisexual european crucian carp (Carassius gibelio (Bloch, 1782)) in northern ukraine: allozyme markers’ and chromosome number’s analysis
SUMMARY. The allozyme analysis of five polymorphic loci sets of gynogenetic prucian carps C. gibelio from Northern Ukraine allowed to identify, within this species, 29 genetic forms of clone and recombinant nature; which can be divided into two categories. The first one is represented by the clone biotype C. gibelio-1 with modal number of 156 chromosomes and a number of unique alleles, which differentiate it distinctly from zooids of C. auratus. The second category is represented by the group consisting of 28 biotypes, designated as C. gibelio-2 of clone and recombinant nature. This group’s fishes have no allozymes, which distinguish them from C. auratus. Within this group the 158 and 160 modal chromosome groups were discovered; each one has special genotypic combination of gene locus Aat-1. Apparently the series of biotypes, which are differentiated by the modal chromosome number, appeared independently as a result of hybridization of different parental species’ couples way back in Eastern Asia. Whereas the appearance of recombinant individuals with unique combinations of genotypes by five protein loci and by chromosome number within the clone C. gibelio-2 is a secondary event and it is related to inheritance mechanism implementation, which combines cloning and mendelian inheritance. There are some reasons to consider that this mechanism is implemented by pseudogamous crossing of genetically close females of C. gibelio-2 with males of C. auratus, while during the crossing of C. gibelio-2 females with C. ñarassius males the cloning takes place.
Key words: ñloning, populations, crucian carps, Carassius, allozymes, chromosomal numbers
Tsitologiya i Genetika 2020, vol. 54, no. 1, pp. 71-79
E-mail: smezhzherin gmail.com
1. Kobayashi, H., Nakano, K., and Nakamura, M., On the hybrids, 4n ginbuna (Carassius auratus langsdorfii) × kinbuna C. auratus subsp.), and their chromosomes, Bull. Japan Soc. Sci. Fish., 1977, vol. 43, no. 301, pp. 31–37.
2. Lin, S.M., Sezaki, K., Hashimoto, K., and Nakamura, M., Distribution of polyploids of “ginbuna” Carassius auratus langsdorfii in Japan, Bull. Jap. Soc. Sci. Fish., 1980, vol. 46, no. 4, pp. 413–418.
3. Shimuzu, Y., Oshiro, O., and Sakaizumi, M., Electrophoretic studies of diploid, triploid, and tetraploid forms of the Japanese silver crucian carp, Carassius auratus langsdorfii,Jap. J. Ichthyol., 1993, vol. 40, no. 1, pp. 65–75.
4. Murakami, M., Matsuda, C., and Fujitani, H., The maternal origins of the triploid ginbuna (Carassius auratus langsdorfii): phylogenetic relationships within the C. auratus taxa by partial mitochondrial D-loop sequencing, Genes Genet. Syst., 2001, vol. 76, no. 1, pp. 25–32. https://doi.org/10.1266/ggs.76.25
5. Cherfas, N.B., Gynogenesis in fishes, in Genetic Basis of Fish Selection, Kirpichnikov, V.S., Ed., Berlin: Springer-Verlag, pp. 255–273.
6. Kottelat, M. and Freyhof, J., Handbook of European Freshwater Fishes, Chester, 2007.
7. Mezhzherin, S.V., Kokodiy, S.V., Kulish, A.V., Verlatiy, D.B., and Fedorenko, L.V., Hybridization of crucian carp (Carassius carassius (Linnaeus, 1758)) in Ukrainian reservoirs and genetic structure of hybrids, Cytol. Genet., 2012, vol. 46, no. 1, pp. 37–46. https://doi.org/10.3103/S0095452712010069
8. Jakovlič, I. and Gui, J.-F., Recent invasion and low level of divergence between diploid and triploid forms of Carassius auratus complex in Croatia, Genetica, 2011, vol. 139, no. 6, pp. 789–804. https://doi.org/10.1007/s10709-011-9584-y
9. Qin, Q., Wang, J., Hu, M., Huang, S., and Liu, S., Autotriploid origin of Carassius auratus as revealed by chromosomal locus analysis, Sci. China Life Sci., 2016, vol. 59, no. is. 6, pp. 622–626. https://doi.org/10.1007/s11427-016-5040-7
10. Cherfas, N.B., Natural tripody in the females of the unisexual variety of the silver crucian carp (Carassius auratus gibelio Aloch), Genetika, 1966, vol. 2, no. 5, pp. 16–24.
11. Vujosevich, M.S., Zivkovic, S., Rimsa, D., Rinsa, D., Jurišič, S., and Cakič, P., The chromosomes of 9 fish species from Dunav basin in Yugoslavia, Ichthyologia, 1983, vol. 15, no. 2, pp. 29–40.
12. Fister, S. and Soldatovic, B., Karyotype analysis of a gynogenetic population of Carassius auratus gibelio, Bloch (Cyprinidae) from Pancevacki Rit, Acta Veterinaria (Beograd), 1989, vol. 39, nos. 5–6, pp. 259–268.
13. Boroń, A., Karyotypes of diploid and triploid silver crucian carp Carassius auratus gibelio (Bloch), Cytobios, 1994, vol. 80, pp. 117–124.
14. Boroń, A., Szlachciak, J., Juchno, D., Grabowska, A., Jagusztyn, B., and Porycka, K., Karyotype, morphology, and reproduction ability of the Prussian carp, Carassius gibelio (Actinopterygii: Cypriniformes: Cyprinidae), from unisexual and bisexual populations in Poland, Acta Ichthyol. Piscat., 2011, vol. 41, no. 1, pp. 19–28. https://doi.org/10.3750/aip2011.41.1.04
15. Mezhzherin, S.V. and Kokodiy, S.V., On the polyphyletic origin of the European triploid crucian carp Carassius gibelio, Rep. Nat. Acad. Sci. Ukr., 2006, no. 7, pp. 169–174.
16. Takada, M., Tachihara, K., Kon, T., and Yamamoto, G., Biogeography and evolution of the Carassius auratus-complex in East Asia, BMC Evol., Biol., 2010, vol. 10, no. 7. https://doi.org/10.1186/1471-2148-10-7
17. Rylková, K., Kalous, L., Bohlen, J., Lamatsch, D.K., and Petrtyl, M., Phylogeny and biogeographic history of the cyprinid fish genus Carassius (Teleostei: Cyprinidae) with focus on natural and anthropogenic arrivals in Europe, Aquaculture, 2013, vol. 380, no. 3, pp. 13–20. https://doi.org/10.1016/j.aquaculture.2012.11.027
18. Toth, B., Varakoni, E., Hidas, A., Meleg, E.E., and Varadi, L., Genetic analysis of offspring from intraand interspecific crosses of Carassius auratus gibelio by chromosome and RAPD analysis, J. Fish Biol., 2005, vol. 66, no. 3, pp. 784–797. https://doi.org/10.1111/j.0022-1112.2005.00644.x
19. Kalous, L. and Knytl, M., Karyotype diversity of the offspring resulting from reproduction experiment between diploid male and triploid female of silver Prussian carp, Carassius gibelio (Cyprinidae, Actiopterygii), Folia Zool., 2011, vol. 60, no. 2, pp. 115–121. https://doi.org/10.25225/fozo.v60.i2.a5.2011
20. Boroń, A., Cytogenetic characterization of the loaches of the genera Sabanejewia, Misgurnus and Barbatula (Pisces, Cobitidae), Folia Zool., 2000, vol. 49, no. 1, pp. 37–44.
21. Ráb, P. and Roth, P., Coldblooded vertebrates, in Methods of Chromosome Analysis, Baliček, P., Forejt, J., and Rubeš, J., Eds., Cytogenet. Sect. Cs. Biol. Soc., 1988, pp. 115–124.
22. Cucchi, C. and Baruffaldi, A.A., New method for karyological studies in teleost fishes, J. Fish. Biol., 1990, vol. 37, no. 1, pp. 71–75.
23. Vasil’eva, V.P., Gold crucian carp, Carassius carassius—diploid or tetraploid species?, Dokl. Akad. Nauk SSSR, 1985, vol. 283, no. 1, pp. 228–230.
24. Mezhzherin, S.V. and Kokodiy, S.V., Genetic structure of silver Prussian carp Carassius (superspecies auratus) (Linnaeus, 1758) colonies from the Middle Dnieper basin, Russ. J. Genet., 2010, vol. 46, no. 6, pp. 725–731.
25. Stock, M., Ustinova, J., Betto-Colliard, C., Schartl, M., Moritz, C., and Perrin, N., Simultaneous Mendelian and clonal genome transmission in a sexually reproducing, all-triploid vertebrate, Proc. Biol. Sci., 2012, vol. 279, no. 1732, pp. 1293–1299. https://doi.org/10.1098/rspb.2011.1738
26. Brykov, V.A., Apalikova, O.V., Eliseikina, M.G., and Kovalev, M.Yu., Mitochondrial DNA variation in diploid and triploid forms of silver crucian carp Carassius auratus gibelio,Russ. J. Genet., 2005, vol. 41, no. 6, pp. 811–816.
27. Szabelska, A., Kirtiklis, L., and Przybył, A., 5S rDNA sequence shows differences between diploid and triploid Prussian carp Carassius gibelio (Teleostei, Cyprinidae), Turk. J. Fish. Aquat. Sci., 2017, vol. 17, no. 6, pp. 1127–1133. https://doi.org/10.4194/1303-2712-v17_6_06
28. Litvinchuk, S. and Rosanov, Yu.M., Natural autotryploidy in the Danube newt Triturus dobrogicus (Salamandridae), Russ. J. Herpetol., 2001, vol. 8, no. 1, pp. 74–76.
29. Litvinchuk, S., Skorikov, D., and Rosanov, Yu.M., Natural spontaneous autotripoloidy in the genus Pelophylax (Anura: Ranidae), Russ. J. Herpetol., 2015, vol. 22, no. 4, pp. 318–320.
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