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Changes in the inflammation state of white adipose tissue in rats with diet-induced obesity at different modes of melatonin administration

Kalmukova O., Yurchenko A., Savchuk A., Dzerzhynsky M.


SUMMARY. The effects of various modes (morning and evening) of melatonin (30 mg/kg/day for 7 weeks) administration on the inflammatory process in visceral white adipose tissue (presence of macrophages forming structures similar to the crown-like structures around adipocytes; the level of fibrosis and the mast cells number in white adipose tissue; the content of proinflammatory (IL-1β, IL-8, tumor necrosis factor α, interferon γ) and anti-inflammatory (IL-4, IL-10) cytokines in blood serum) in rats with diet-induced obesity was investigated. After the administration of melatonin, obese rats demonstrated the decrease of macrophages and mast cells number in white adipose tissue, with a notable reduction of collagen fiber content (fibrosis) and adipocyte cell area. Evening administration of melatonin (one hour before light-off) showed greater efficacy compared to morning (one hour after light-on). In addition, serum of obese rats which received melatonin had increased levels of anti-inflammatory cytokines, while pro-inflammatory on the contrary decreased. This indicates about manifestation of the anti-inflammatory properties of melatonin in the context of the obesity development and is a theoretical precondition for further consideration of the possibility to use it in the obesity treatment.

Key words: high calorie obesity, inflammation, chronobiology, melatonin, cytokines, fibrosis, mast cells, adipocytes

Tsitologiya i Genetika 2020, vol. 54, no. 1, pp. 50-61

  • Educational and scientific centre Institute of Biology and Medicine Taras Shevchenko National University of Kyiv 64/13, Volodymyrska St., 01601 Kyiv, Ukraine

E-mail: olesiakalmukova28

Kalmukova O., Yurchenko A., Savchuk A., Dzerzhynsky M. Changes in the inflammation state of white adipose tissue in rats with diet-induced obesity at different modes of melatonin administration, Tsitol Genet., 2020, vol. 54, no. 1, pp. 50-61.

In "Cytology and Genetics":
O. O. Kalmukova, A. V. Yurchenko, A. M. Savchuk & M. E. Dzerzhynsky Changes in the Inflammatory Status in White Adipose Tissue of Rats with Diet-Induced Obesity at Different Regimens of Melatonin Administration, Cytol Genet., 2020, vol. 54, no. 1, pp. 3847
DOI: 10.3103/S0095452720010077


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