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Comparative analysis of the genetic structure of paddlefish (Polyodon spathula) populations by microsatellite DNA-markers
SUMMARY. The comparative analysis of the genetic structure of artificial Ukrainian and Polish populations with natural paddlefish populations from the United States was conducted using three microsatellite DNA markers: Psp21, Psp26 and Psp28. The average value of Na was 6.1 and 5.5 for the Ukrainian and Polish populations, respectively. For natural populations Na index was almost twice as high and averaged at 11.1 alleles. It was established that there was predominance of the mean values of the observed heterozygosity (Íî) over expected heterozygosity (Íe) both for the Ukrainian (0.709 > 0.616) and for the Polish (0.809 > 0.699) populations. For natural populations, the mean values of Ho and He were close to the Hardy-Weinberg Equilibrium (HWE) and were at the level of 0.817 and 0.813, respectively. According to comparable data, it has been established that there has been a decrease in the total number of allelic variants for artificial populations, compared with natural populations. The obtained values of the level of heterozygosity and the negative fixation indexes Fis for artificial paddlefish populations indicated the absence of inbreeding at this stage of paddlefish cultivation, which was a confirmation of a sufficient number of individuals in broodstock with heterozygous genotypes for reproduction under aquaculture conditions.
Key words: Polyodon spathula, DNA-markers, microsatellites, genetic structure, alleles, loci, polymorphism
Tsitologiya i Genetika 2020, vol. 54, no. 1, pp. 42-49
E-mail: khrystyna.kurta gmail.com, malisheva.sirota gmail.com, spyrydonov ukr.net
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