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omparative genetic characteristics of the russian and belarusian populations of wisent (Bison bonasus), north american bison (Bison bison) and cattle (Bos taurus)

Kostyunina O.V., Mikhailova M.E., Dotsev A.V., Zemlyanko I.I., Volkova V.V., Fornara M.S., Akopyan N.A., Kramarenko A.S., Okhlopkov I.M., Aksenova P.V., Tsibizova E.L., Mnatsekanov R.A., Zinovieva N.A.

 




SUMMARY. A comparative study of the allele pool and the genetic structure of two Russian and one Belarusian populations of the wisent Bison bonasus against the representatives of the species of Bison bison and the genus Bos taurus was carried out. Russian populations were represented by samples of the Oka State Natural Biosphere Reserve (n = 42) and the Prioksko-Terrasny Nature Biosphere Reserve (n = 69), the Belarusian population was represented by samples of the Reserve Belovezhskaya Pushcha (n = 42), bison samples (n = 8) and cattle (n = 55) were used as an outgroup. The analysis of the mtDNA D-loop 630 bp fragment polymorphism for the presence of bison and cattle haplotypes, was performed. It was shown that there was a single haplotype for all wisent (Bison bonasus) which was different from the sequences of bison (Bison bison) and cattle. The analysis of population genetic parameters, calculated using 11 microsatellite markers, showed reduced diversity in wisent (Bison bonasus) groups compared to bison (Bison bison) and cattle. The largest number of monomorphic loci as well as the absence of private alleles were found in the group of Belarusian wisent. The analysis of the DJost pairwise genetic distances allowed us to establish clear genetic differentiation of the wisent from the outgroups. This fact was also confirmed by PCA analysis, carried out in the context of population identity and the analysis of population structure which demonstrated some proximity of bison (Bison bison) to wisent (Bison bonasus), which is due to their belonging to the same genus. The data obtained from this study showed differentiation between wisent (Bison bonasus) from bison (Bison bison) and cattle. It can be used in assessing the population genetic parameters of wisent, identifying and eliminating hybrid individuals. It also can be used in developing strategies and measures for the preservation and improvement of the wisent genetic resources.

Key words: wisent (European bison), Bison bonasus, bison (North American bison; Bison bison), Bos taurus, mtDNA, microsatellites; allele pool

Tsitologiya i Genetika 2020, vol. 54, no. 2, pp. 35-44

  1. Federal Science Center for Animal Husbandry named after Academy Member L.K. Ernst, 142132, Dubrovitsy, Russian Federation
  2. The Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, 220072, Minsk, Belarus
  3. Prioksko-Terrasny State Reserve, 142200, Danki, Russian Federation
  4. Mykolayiv National Agrarian University, 54020, Mykolayiv, Ukraine
  5. Institute for Biological Problems of Cryolithozone Siberian Branch of RAS, 677980, Yakutsk, Russian Federation
  6. Don State Technical University (DSTU), 344000, Rostov-on-Don, Russian Federation
  7. Federal State Institution Oksky State Natural Biosphere Reserve, 391072, Brykin Bor, Russian Federation
  8. World Wide Fund for Nature (WWF Russia) 109240, Moscow, Russian Federation

E-mail: kostolan yandex.ru, m.mikhailova igc.by, arsendotsev vij.ru, zemlyankoirina yandex.ru, moonlit_elf mail.ru, margaretfornara gmail.com, kssnail1990 gmail.com, imokhlopkov yandex.ru, polinax1 mail.ru, etzibizova mail.ru, RMnatsekanov wwf.ru, n_zinovieva ma

Kostyunina O.V., Mikhailova M.E., Dotsev A.V., Zemlyanko I.I., Volkova V.V., Fornara M.S., Akopyan N.A., Kramarenko A.S., Okhlopkov I.M., Aksenova P.V., Tsibizova E.L., Mnatsekanov R.A., Zinovieva N.A. omparative genetic characteristics of the russian and belarusian populations of wisent (Bison bonasus), north american bison (Bison bison) and cattle (Bos taurus), Tsitol Genet., 2020, vol. 54, no. 2, pp. 35-44.

In "Cytology and Genetics":
Kostyunina, M. E. Mikhailova, A. V. Dotsev, I. I. Zemlyanko, V. V. Volkova, M. S. Fornara, N. A. Akopyan, A. S. Kramarenko, I. M. Okhlopkov, P. V. Aksenova, E. L. Tsibizova, R. A. Mnatsekanov & N. A. Zinovieva Comparative Genetic Characteristics of the Russian and Belarusian Populations of Wisent (Bison bonasus), North American Bison (Bison bison) and Cattle (Bos taurus), Cytol Genet., 2020, vol. 54, no. 2, pp. 116123
DOI: 10.3103/S0095452720020085


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