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Correlation between nucleotide substitutions in the glycerol-3-phosphate oxidase gene, the level of hydrogen peroxide production and cytotoxicity of Mycoplasma pneumoniae
SUMMARY. Mycoplasma pneumoniae is the etiological agent of various forms of human respiratory pathology: sinusitis, pharyngitis, laryngitis, bronchitis, pneumonia, bronchial asthma. One of the main pathogenic factors of Mycoplasma pneumoniae, hydrogen peroxide, is released in the enzymatic reaction catalyzed by glycerol-3-phosphate (G3P) oxidase. In the G3P oxidase gene of Mycoplasma pneumoniae clinical isolates, obtained from the biological material of patients with bronchitis and pneumonia of mycoplasma etiology, A152T (His51Leu) and G163C (Asp55His) substitutions have been identified, associated with the variations in enzyme activity. Mycoplasma pneumoniae isolates, carrying the A152T substitution (His51Leu), produced hydrogen peroxide significantly lower (5 mg/l) in comparison with the reference strain (10 mg/l) and had reduced cytotoxicity to respiratory epithelial cells. While Mycoplasma pneumoniae isolates, carrying substitution G163C (Asp55His), were characterized by enhanced pathogenic properties, such as increased production of hydrogen peroxide (25 mg/l) and more pronounced cytotoxicity to respiratory epithelial cells.
Key words: Mycoplasma pneumoniae, glycerol-3-phosphate oxidase, nucleotide substitutions, hydrogen peroxide, cytotoxicity
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|Coded & Designed by Volodymyr Duplij||Modified 22.06.21|