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Genetic diversity of sunflower inbred lines of Kazakhstan collection fund on the protein and SSR markers
SUMMARY. The article presents the results of the study on genetic diversity of 126 sunflower inbred lines from «Experimental farm of Oilseeds» of Kazakhstan collection fund by protein and SSR markers. The polymorphism of collections in composition of helianthinin was not high, in the zone of fast migrated proteins three variants of components were found that are characteristic of all types of inbred lines. At the same time, components specific to lines with rf and Rf genes have been identified. PCR analysis of inbred lines DNA by 16 SSR markers showed no variations in the amplification products of the ORS 428 locus, low polymorphism of the ORS 785 locus (PIC ≤ 0.2). High polymorphism (PIC > 0.8) in the studied collections was demonstrated by the following markers: ORS316, ORS381, ORS 437, ORS456, ORS505, ORS, ORS630, ORS761, ORS925. Based on molecular marking, registration of inbred lines of Kazakhstan collection fund was carried out. In order to assess the level of hybridity of F1 seeds of commercial sunflower hybrids, SSR markers were selected that can differentiate the genetic material of the original parental lines in the DNA of hybrid.
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|Coded & Designed by Volodymyr Duplij||Modified 22.06.21|