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Genetic diversity of sunflower inbred lines of Kazakhstan collection fund on the protein and SSR markers

Bulatova K.M., Mazkirat Sh., Gavrilova O.A., Yusaeva D.A., Babissekova D.I., Alchinbayeva P.A.

 




SUMMARY. The article presents the results of the study on genetic diversity of 126 sunflower inbred lines from Experimental farm of Oilseeds of Kazakhstan collection fund by protein and SSR markers. The polymorphism of collections in composition of helianthinin was not high, in the zone of fast migrated proteins three variants of components were found that are characteristic of all types of inbred lines. At the same time, components specific to lines with rf and Rf genes have been identified. PCR analysis of inbred lines DNA by 16 SSR markers showed no variations in the amplification products of the ORS 428 locus, low polymorphism of the ORS 785 locus (PIC ≤ 0.2). High polymorphism (PIC > 0.8) in the studied collections was demonstrated by the following markers: ORS316, ORS381, ORS 437, ORS456, ORS505, ORS, ORS630, ORS761, ORS925. Based on molecular marking, registration of inbred lines of Kazakhstan collection fund was carried out. In order to assess the level of hybridity of F1 seeds of commercial sunflower hybrids, SSR markers were selected that can differentiate the genetic material of the original parental lines in the DNA of hybrid.

Tsitologiya i Genetika 2020, vol. 54, no. 1, pp. 16-26

  1. Kazakh Scientific Research Institute of Agriculture and Plant Growing, Almaty region, Kazakhstan
  2. Experimental farm of oilseeds, Solnechnoye v., Glubokovskiy distr., Eastern Kazakhstan region, Kazakhstan

E-mail: bulatova_k rambler.ru

Bulatova K.M., Mazkirat Sh., Gavrilova O.A., Yusaeva D.A., Babissekova D.I., Alchinbayeva P.A. Genetic diversity of sunflower inbred lines of Kazakhstan collection fund on the protein and SSR markers, Tsitol Genet., 2020, vol. 54, no. 1, pp. 16-26.

In "Cytology and Genetics":
K. M. Bulatova, Sh. Mazkirat, O. A. Gavrilova, D. A. Yusaeva, D. I. Babissekova & P. A. Alchinbayeva Genetic Diversity of Inbred Sunflower Lines of the Kazakhstan Collection Fund for Protein and SSR Markers, Cytol Genet., 2020, vol. 54, no. 1, pp. 1017
DOI: 10.3103/S009545272001003X


References

1. Poperelya, F.O., Genetic interpretation of electrophoregrams of helianthinin from sunflower F1 seeds, Cytol. Genet., 2000, vol. 34, no. 2, pp. 8490.

2. Bochkovoy, A.D., Antonova, T.S., Kamardin, V.A., Araslanova, N.M., Veter, I.I., Guchetl, S.Z., and Chelyustnikova, T.A., The efficiency of different methods of determination of the genetic purity of the seed lots of selfpollinated lines and hybrids of the F1 generation of sunflower, Oil Crops. Sci. Techn. Bull. VNIIMK, 2014, vol. 1578, no. 1, pp. 15.

3. Aksyonov, I.V. and Mishchenko, L., Use of electrophoretic spectra of reserve seed proteins to improve typicalness of sunflower parental lines, Russ. Agric. Sci., 2016, vol. 42, nos. 34, pp. 211214. https://doi.org/10.3103/S1068367416030034

4. Sivolap, Y.M., Molecular markers and plant breeding, Cytol. Genet., 2013, vol. 47, no. 3, pp. 188195. https://doi.org/10.3103/S0095452713030080

5. Paniego, N., Echaide, M., Munoz, M., Fernandez, L., Torales, S., Faccio, P., Fuxan, I., Carrera, M., Zandomeni, R., Suarez, E.Y., and Hopp, H.E., Microsatellite isolation and charaterization in sunflower (Helianthus annuus L.), Genome, 2002, vol. 45, no. 1, pp. 3443. https://doi.org/10.1139/G01120

6. Yu, J.K., Mangor, J., Thompson, L., Edwards, K.J., Slabaugh, M.B., and Knapp, S.J., Allelic diversity of simple sequence repeat markers among elite inbred lines in cultivated sunflower, Genome, 2002, vol. 45, no. 4, pp. 652660. https://doi.org/10.1139/g02025

7. Tang, S., Yu, J.K., Slabaugh, M.B., Shintani, D.K., and Knapp, S.J., Simple sequence repeat map of the sunflower genome, Theor. Appl. Genet., 2002, vol. 105, no. 8, pp. 11241136. https://doi.org/10.1007/s001220020989y

8. Mandel, J.R., Dechaine, J.M., Marek, L.F., and Burke, J.M., Genetic diversity and population structure in cultivated sunflower and a comparison to its wild progenitor, Helianthus annuus L., Theor. Appl. Genet., 2011, vol. 123, no. 5, pp. 693704. https://doi.org/10.1007/s0012201116193

9. Zeinalzadeh-Tabrizi, H., Haliloglu, K., Ghaffari, M., and Hosseinpour, A., Assessment of genetic diversity among sunflower genotypes using microsatellite markers, Mol. Biol. Res. Commun., 2018, vol. 7, no. 3, pp. 14352.

10. Pankovic, D., Application of molecular markers if sunflower breeding, Genetika, 2007, vol. 39, no. 1, pp. 111. https://doi.org/10.2298/GENSR0701001S

11. Imerovski, I., Dimitrijevic, A., Miladinovic, D., Dedic, D., Jocic, S., and Miklic, V., Molecular profiles of sunflower lines resistant to broomrape (Orobanche numana Wallr.), in Proc. Int. Symp. on Broomrape (Orobanche spp.) in Sunflower. Chisinau, Republic of Moldova, 2011, p. 25.

12. Duca, M., Port, A., Midoni, A., Rotaru, T., and Anisimova, I., RAPD analysis of the genetic variation among CMS, Rf lines and hybrid sunflower genotypes, J. Acad. Sci. Moldova,Life Sci., 2010, vol. 2, no. 31, pp. 103109.

13. Duca, M., Port, A., and Şestacova, T., Screening of the R2 rust resistance gene in different sunflower genotypes using SSR markers. J. Acad. Sci. Moldova,Life Sci., 2011, vol. 2, no. 314, pp. 106110.

14. Port, A., Nechifor, V., Midoni, A., and Duca, M., Usefulness of the diagnostic markers for the restorergene Rf1 in inheritance studies at sunflower, Ann. Alexandru Ioan Cuza Univ., Sect. II.a Genetics Mol.Biol., 2013, vol. 14, no. 2, pp. 1119.

15. Markin, N.V., Tikhobaeva, V.E., Tikhonova, M.A., Gavrilova, V.A., Tolstaya, T.T., and Usatov, A.V., Genomic DNA polymorphism in annual sunflower species, Oil Crops. Sci. Techn. Bull. VNIIMK, 2010, vol. 2, no. 1445, pp. 37. https://doi.org/10.3844/ajbbsp.2014.136.140

16. Guchetl, S.Z., Tchelustnikova, T.A., and Antonova, T.S., Certification of new sunflower lines and hybrids of VNIIMK breeding by means of biochemical and molecular markers, Oil Crops. Sci. Techn. Bull. VNIIMK, 2015, vol. 163, no. 3, pp. 1623.

17. Usatov, A.V., Gorbachenko, F.I., Azarin, K.V., Gorbachenko, O.F., Tikhobaeva, V.E., and Markin, N.V., The relation between the heterosis effect of hybrids and genetic distance of sunflower parental lines vol, Oil Crops. Sci. Techn. Bull. VNIIMK, 2013, vol. 155-6, no. 2, pp. 813.

18. Usatov, A.V., Klimenko, A.I., Azarin, K.V., Gorbachenko, O.F., Markin, N.V., Tikhobaeva, V.E., Kolosov, Yu.A., Usatova, O.A., Bakoev, S., Makarenko, M., and Getmantseva, A., The relationship between heterosis and genetic distances based on SSR markers in Helianthus annuus,Am. J. Agric. Biol. Sci., 2014, vol. 9, no. 3, pp. 270276. https://doi.org/10.3844/ajabssp.2014.270.276

19. Markin, N.V., Gorbachenko, O.F., Tikhonova, M.A., and Usatov, A.V., Condominant markers of rf1 gene in cultivated sunflower, Oil Crops. Sci. Techn. Bull. VNIIMK, 2010, vol. 1423, no. 1, pp. 38.

20. Galili, G. and Feldman, M., Genetic control of endosperm proteins in wheat. 2. Variation in high molecular weight glutenin and gliadin subunits of Triticum aestivum,Theor. Appl. Genet.,1983, vol. 66, no. 1, pp. 7786. https://doi.org/10.1007/BF00281853

21. Kimura, M. and Crow, J.F., The number of alleles that can be maintained in a finite population, Genetics, 1964, vol. 49, no. 4, pp. 72538.

22. Nei, M., Analysis of gene diversity in subdivided populations, Proc. Natl. Acad. Sci. U. S. A., 1973, vol. 70, pp. 33213323.

23. Lewontin, R.C., Testing the theory of natural selection, Nature, 1972, vol. 236, pp. 181182.https://doi.org/10.1038/236181a0

24. Yeh, F.C. and Boyle, T.J.B., Population genetic analysis of codominant and dominant markers and quantitative traits, Belgian J. Bot., 1997, vol. 129, pp. 157163.

25. Žilić, S., Barać, M., Pešić, M., Crevar, M., Stanojević, S., Nišavić, A., Saratlić, G., and Tolimir, M., Characterization of sunflower seed and kernel proteins, Helia, 2010, vol. 33, no. 52, pp. 103114. https://doi.org/10.2298/HEL1052103Z

26. Nikolić, Z., Vujaković, and Jevtić, Genetic purity of sunflower hybrids Determined on the basis of isozymes and seed storage proteins, Helia, 2008, vol. 31, no. 48, pp. 4754. https://doi.org/10.2298/HEL0848047N

27. Anisimova, I.N., Proteins of Compositaes seeds: heterogeneity, poly-morphism, usage in selection and genetic researches (review), Agrarn. Rossiya, 2015, vol. no, pp. 2735.

28. Zhang, L., Le Clerc V., Li S., and Zhang D. Establishment of an effective set of simple sequence repeat markers for sunflower variety identification and diversity assessment, Can. J. Bot., 2005, vol. 83, no. 1, pp. 6672. https://doi.org/10.1139/b04155

29. Hvarleva, Tz., Bakalova, A., Chepinski, I., Hristova-Cherbadji, M., Hristov, M., and Atanasov, A., Characterization of Bulgarian sunflower cultivars and inbred lines with microsatellite markers, Biotechnol. Biotechnol. Eq., 2007, vol. 21, no. 4, pp. 408412.https://doi.org/10.1080/13102818.2007.10817484

30. Dimitrijević, A., Imerovski, I., Miladinović, D., Tančić, S., Dušanić, N., Jocić, S., and Miklič, V., Use of SSR markers in identification of sunflower isogenic lines in late generations of backcrossing, Helia, 2010, vol. 33, no. 53, pp. 191198. https://doi.org/10.2298/HEL1053191D

31. Duca, M., Port, A., Şestacova, T., Siniauskaya, M., Aksyonova, E., and Davydenko, O., Microsatellite marker application in sunflower (Helianthus annuus L.) Fingerprinting,Biotechnol. Biotechnol. Eq., 2013, vol. 27, no. 3, pp. 37723775. https://doi.org/10.5504/BBeQ.2013.0021

32. Pallavi, H.M., Gowda, R., Shadakshari, Y.G., Bhanuprakash, K., and Vishwanath, K., Identification of SSR markers for hybridity and seed genetic purity testing in sunflower (Helianthus annuus L.), Helia, 2011, vol. 34, no. 54, pp. 5966. https://doi.org/10.2298/HEL1154059P

33. Sudharani, M., Rao, P.S., and Subba, RaoL.V., Identification of SSR markers for testing of hybridity and seed genetic purity in maize (Zea mays L.), Int. J. Sci. Res., 2014, vol. 3, no. 10, pp. 9295.

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