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Reproductive function of cows with different genotype by locus TNFα and estimating the fertility of sperm cells by DNA fragmentation method

Bimenova Zh.Zh., Ussenbekov Ye.S., Terletskiy V.P., Makashev Ye.K., Tyshchenko V.I., Kassymbekova Sh.N.


SUMMARY. SNP polymorphism of the promoter region of the TNFα gene at position 824 A→G in the Holstein cows of Bayserke-Agro LLP is represented by the following genetic variants: AA 22,4 %, AG 63,8 %, GG 13,8 %, the frequency of alleles A and G was 0,54 and 0,46, respectively. In population under this study, the excessive number of the heterozygous genotype AG reached the value of 21,49, and in other genotypes there was a deficit of homozygous variants of GG and AA, by 11,16 and 10,32 individuals respectively. The traits of reproductive function were high in cows with GG genotype: the interval between calving and productive insemination was 259 days, the insemination index was 2,63, the proportion of animals inseminated after more than 91 days was at minimum value of 47,36 % in individuals with homozygous genotype GG. As an additional criterion for assessing the fertility of bull sperm, the DNA fragmentation method is recom-mended, the maximum permissible level of sperm cells with DNA fragmentation in frozen sperm is between 13,00 and 17,15 %.

Key words: promoter region of the TNFα gene, PCR-RFLP, reproductive function of cows, DNA fragmentation, bull sperm fertility

Tsitologiya i Genetika 2019, vol. 53, no. 1, pp. 51-58

E-mail: zhan_225, usen03, valeriter, e_makashev, tinatvi, kasymbekovas

Bimenova Zh.Zh., Ussenbekov Ye.S., Terletskiy V.P., Makashev Ye.K., Tyshchenko V.I., Kassymbekova Sh.N. Reproductive function of cows with different genotype by locus TNFα and estimating the fertility of sperm cells by DNA fragmentation method, Tsitol Genet., 2019, vol. 53, no. 1, pp. 51-58.

In "Cytology and Genetics":
Zh. Zh. Bimenova, Ye. S. Ussenbekov, V. P. Terletskiy, Ye. K. Makashev, V. I. Tyshchenko, Sh. N. Kassymbekova Reproductive Function of Cows with Different Genotypes for TNFα Locus and Estimation of Sperm Fertility by the DNA Fragmentation Method, Cytol Genet., 2019, vol. 53, no. 1, pp. 4248
DOI: 10.3103/S0095452719010043


1. Agaba, M., Kemp, S.J., Barendse, W., and Teale, A.J., Polymorphism at the bovine tumor necrosis factor alpha locus and assignment to BTA 23, Mamm. Genome, 1996, vol. 7, pp. 186187.

2. Kawasaki, Y., Aoki, Y., Magata, F., Miyamoto, A., Kawashima, C., Hojo, T., Okuda, K., Shirasuna, K., and Shimizu, T., The effect of single nucleotide polymorphisms in the tumor necrosis factor-α gene on reproductive performance and immune function in dairy cattle, J. Reprod. Dev., 2014, vol. 60, no. 3, pp. 173178.

3. Bojarojc-Nosowicz, B., Brym, P., Kaczmarczyk, E., Stachura, A., and Habel, A.K., Polymorphism and expression of the tumour necrosis factor-alpha (TNF α) gene in non-infected cows and in cows naturally infected with the bovine leukaemia virus (BLV), Vet. Med. (Prague, Czech Repub.), 2016, vol. 61, no. 1, pp. 19. 4. Martel, C.A., Mamedova, L.K., Minton, J.E., Garcia, M., Legallet, C., and Bradford, B.J., Effects of TNF receptor blockade on in vitro cell survival and response to negative energy balance in dairy cattle, J. Anim. Sci. Biotechnol., 2018, vol. 9, p. 6.

5. Davies, D., Meade, K.G., Herath, S., Eckersall, P.D., Gonzalez, D., White, J.O., Conlan, R.S., OFarrelly, C., and Sheldon, I.M., Toll-like receptor and antimicrobial peptide expression in the bovine endometrium, Reprod. Biol. Endocrinol., 2008, vol. 6, p. 53.

6. Brayman, M., Thathiah, A., and Carson, D.D., MUC1: a multifunctional cell surface component of reproductive tissue epithelia, Reprod. Biol. Endocrinol., 2004, vol. 2, pp. 19.

7. Silva, A.W.B., Bezerra, F.T.G., Glanzner, W.G., Santos, J.T., Dau, A.M.P., Rovani, M.T., Ilha, G.F., Costa, J.J.N., Cunha, E.V., Donato, M.A.M., Peixoto, C.A., Goncalves, P.B.D., Bordignon, V., and Silva, J.R.V., mRNA expression profile of the TNF α system in LH-induced bovine preovulatory follicles and effects of TNF α on gene expression, ultrastructure and expansion of cumulus-oocyte complexes cultured in vitro, Theriogenology, 2017, vol. 90, pp. 110.

8. Flowers, W.L., Sperm characteristics that limit success of fertilization, J. Anim., 2013, vol. 91, no. 7, pp. 30223029.

9. Karoui, S., Díaz, C., González-Marín, C., Amenabar, M.E., Serrano, M., Ugarte, E., Gosálvez, J., Roy, R., Lopez-Fernández, C., and Carabano, M.J., Is sperm DNA fragmentation a good marker for field AI bull fertility, J. Anim. Sci., 2012, vol. 90, pp. 243749.

10. Takeda, K., Uchiyama, K., Kinukawa, M., Tagami, T., Kaneda, M., and Watanabe, S., Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality, J. Reprod. Dev., 2015, vol. 61, no. 3, pp. 18590.

11. Evenson, D.P., The Sperm Chromatin Structure Assay (SCSA) and other sperm DNA fragmentation tests for evaluation of sperm nuclear DNA integrity as related to fertility, Anim. Reprod. Sci., 2016, vol. 169, pp. 5675.

12. Bojarojc-Nosowicz, B., Kaczmarczyk, E., Stachura, A., and Kotkiewicz, M., Polymorphism in the promoter region of the tumor necrosis factor-alpha gene in cattle herds naturally infected and uninfected with the Bovine Leukemia Virus, Pol. J. Vet. Sci., 2011, vol. 14, pp. 6713.

13. Cole, J.B., Null, D.J., and Van Raden, P.M., Phenotypic and genetic effects of recessive haplotypes on yield, longevity, and fertility, J. Dairy Sci., 2016, vol. 99, no. 9, pp. 727488.

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