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Embryonic and induced pluripotent stem cells and their differentiation towards cardiomyocytes in presence of DMSO
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SUMMARY. Cell transplantation is a modern strategy for injured heart tissue regeneration. One of promising sources of cardiomyocytes is embryonic stem cells (ESCs) and induced pluripotent stem cells (IPSCs). Genetically modified murine ESC and IPSC expressing green fluorescent protein (GFP) under the control of a cardio specific α-MHC promoter were used for the research; differentiation was carried out in the suspension culture with constant stirring. The effect of dimethylsulfoxide (DMSO) on the processes of differentiation of pluripotent stem cells into cardiomyocytes was studied both at the stage of mesoderm cells formation and at the stage of the cardiomyocytes progenitors formation. Optimal term of DMSO application into the culture was determined. It was established that the addition of DMSO from the 5th to the 9th day of cultivation increased the efficiency of cardiomyocytes differentiation by one and a half times.
Key words: embryonic stem cells, induced pluripotent stem cells, cardiomyocytes, dimethylsulfoxide, differentiation
E-mail: galina19 ukr.net, nbilko ukma.kiev.ua
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