The influence of recombinant interferon α2β synthesized in plants on the reparative enzyme mgmt expression in human somatic cells in vitro

Nidoieva Z.M., Peterson A.A., Ruban T.P., Dzuba G.V., Kuchuk M.V., Lukash L.L.

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SUMMARY. The influence of recombinant interferon α2β synthe-sized in trasgenic plants on the expression of human repair protein O⁶-methylguanine-DNA methyltransfe-rase (MGMT) in both tumour and non-tumour ori-ginated cells was investigated. Human tumour Hеp-2 cells (epidermoid carcinoma of the larynx) and non-tumour human E8 cells (originated in our laboratory from embrionic germ cells) were treated with purified recombinant interferon α2β in serum-free medium. Protein levels were determined by Western-blot method. Recombinant interferon α2β caused decrease in MGMT protein amount in tumour Hеp-2 cells at all treated concentrations (2, 20, 200, 2000 IU/ml) relatively to the control level. In human non-tumour cells E8 we revealed a decrease in MGMT amount at two of the highest concentrations: 200 and 2000 IU/ml, although a statistically significant effect compared to the control level was observed only at a concentration of 200 IU/ml.

Tsitologiya i Genetika 2019, vol. 53, no. 6, pp. 36-43

E-mail: zarinanidoeva i.ua, lukash imbg.org.ua, nkuchuk icbge.org.ua

Nidoieva Z.M., Peterson A.A., Ruban T.P., Dzuba G.V., Kuchuk M.V., Lukash L.L. The influence of recombinant interferon α2β synthesized in plants on the reparative enzyme mgmt expression in human somatic cells in vitro, Tsitol Genet., 2019, vol. 53, no. 6, pp. 36-43.

In "Cytology and Genetics":
Z. M. Nidoieva, A. A. Peterson, T. P. Ruban, G. V. Dzuba, M. V. Kuchuk, L. L. Lukash The Influence of Recombinant Interferon α2b Synthesized in Plants on the Reparative Enzyme MGMT Expression in Human Somatic Cells in vitro, Cytol Genet., 2019, vol. 53, no. 6, pp. 467–472
DOI: 10.3103/S0095452719060070

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