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Expression analysis of α-tubulin genes during cold acclimation in winter and spring soft wheat
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SUMMARY. The expression profiles of 15 α-tubulin genes in spring and winter varieties of common wheat during cold acclimation were studied. Among the studied genes, two subfamilies were identified (3 genes in each) with elevated expression levels detected at the initial stages of cold acclimation. In particular, the Tuba-2-3 gene, which, within its subfamily, is characterized by the most pronounced amplitude of the initial expression level increase. Also the greatest differences in expression levels between varieties were found for this gene within its subfamily. In the case of winter varieties, higher values of expression levels for this tubulin gene were detected, that persist for a long time (up to the 7th day of acclimation). A significant initial increase in expression levels for all α-tubulin genes of the 4th subfamily was registered, reaching maximum values during further acclimation. The high initial values of the expression levels of this subfamily genes may also indicate their important role in resistance ensuring of wheat microtubules to low temperatures in the early stages of cold acclimation.
Key words: Triticum aestivum L., alpha-tubulin, gene expression, cold acclimation
E-mail: denisbuy90 gmail.com, blume.yaroslav nas.gov.ua; demand.dn gmail.com, yarvp1 gmail.com
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