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Expression analysis of α-tubulin genes during cold acclimation in winter and spring soft wheat

Buy D.D., Demkovich A.E., Pirko Ya.V., Blume Ya.B.


SUMMARY. The expression profiles of 15 α-tubulin genes in spring and winter varieties of common wheat during cold acclimation were studied. Among the studied genes, two subfamilies were identified (3 genes in each) with elevated expression levels detected at the initial stages of cold acclimation. In particular, the Tuba-2-3 gene, which, within its subfamily, is characterized by the most pronounced amplitude of the initial expression level increase. Also the greatest differences in expression levels between varieties were found for this gene within its subfamily. In the case of winter varieties, higher values of expression levels for this tubulin gene were detected, that persist for a long time (up to the 7th day of acclimation). A significant initial increase in expression levels for all α-tubulin genes of the 4th subfamily was registered, reaching maximum values during further acclimation. The high initial values of the expression levels of this subfamily genes may also indicate their important role in resistance ensuring of wheat microtubules to low temperatures in the early stages of cold acclimation.

Key words: Triticum aestivum L., alpha-tubulin, gene expression, cold acclimation

Tsitologiya i Genetika 2019, vol. 53, no. 1, pp. 29-40

E-mail: denisbuy90, blume.yaroslav; demand.dn, yarvp1

Buy D.D., Demkovich A.E., Pirko Ya.V., Blume Ya.B. Expression analysis of α-tubulin genes during cold acclimation in winter and spring soft wheat, Tsitol Genet., 2019, vol. 53, no. 1, pp. 29-40.

In "Cytology and Genetics":
D. D. Buy, A. E. Demkovych, Y, a. V. Pirko, Y, a. B. Blume Analysis of α-Tubulin Gene Expression During Cold Acclimation of Winter and Spring Soft Wheat, Cytol Genet., 2019, vol. 53, no. 1, pp. 2333
DOI: 10.3103/S0095452719010067


1. Guo, X., Liu, D., and Chong, K., Cold signaling in plants: Insights into mechanisms and regulation, J. Integr. Plant Biol., 2018, vol. 60, no. 9, pp. 745756.

2. Miura, K. and Furumoto, T., Cold signaling and cold response in plants, Int. J. Mol. Sci., 2013, vol. 14, no. 3, pp. 53125337.

3. Tardif, G., Kane, N.A., Adam, H., Labrie, L., Major, G., Gulick, P., Sarhan, F., and Lalibert, J.F., Interaction network of proteins associated with abiotic stress response and development in wheat, Plant Mol. Biol., 2007, vol. 63, no. 5, pp. 703718.

4. Xiong, L., Schumaker, K.S., and Zhu, J.K., Cell signaling during cold, drought, and salt stress, Plant Cell, 2002, vol. 14, pp. 165183.

5. Yadav, S.K., Cold stress tolerance mechanisms in plants. A review, Agron. Sustain. Dev., 2010, vol. 30, no. 3, pp. 515527.

6. Orvar, B.L., Sangwan, V., Omann, F., and Dhindsa, R.S., Early steps in cold sensing by plant cells: the role of actin cytoskeleton and membrane fluidity, Plant J., 2000, vol. 23, no. 6, pp. 785794.

7. Plohovska, S.H., Krasylenko, Y.A., and Yemets, A.I., Nitric oxide modulates actin filament organization in Arabidopsis thaliana primary root cells at low temperatures, Cell Biol. Int., 2018. 8. Plohovska, S.G., Yemets, A.I., and Blume, Ya.B., Influence of cold on organization of actin filaments of different types of root cells in Arabidopsis thaliana, Cytol. Genet., 2016, vol. 50, no. 5, pp. 318323.

9. Pokorna, J., Sites of actin filament initiation and reorganization in cold treated tobacco cells, J. Plant Cell Environ., 2004, vol. 27, no. 5, pp. 641653.

10. Abdrakhamanova, A., Wang, Q.Y., Khokhlova, L., and Nick, P., Is microtubule disassembly a trigger for cold acclimation?, Plant Cell Physiol., 2003, vol. 44, no. 7, pp. 676686.

11. Kerr, G.P. and Carter, J.V., Relationship between freezing tolerance of root-tip cells and cold stability of microtubules in rye (Secale cereale L. cv. Puma), Plant Physiol., 1990, vol. 93, no. 1, pp. 7782.

12. Sheremet, Y.A., Yemets, A.I., and Blume, Y.B., Inhibitors of tyrosine kinases and phosphatases as a tool for the investigation of microtubule role in plant cold response, Cytol. Genet., 2012, vol. 46, no. 1, pp. 18.

13. Sproule, K., Microtubule involvement in the plant low temperature response, PhD Thesis, University of Saskatchewan, Saskatoon, 2008.

14. Jian, L.C., Sun, L.H., and Liu, Z.P., Studies on microtubule cold stability in relation to plant cold hardiness, Acta Bot. Sinica, 1989, vol. 31, pp. 737741.

15. Bartolo, M.E. and Carter, J.V., Microtubules in the mesophyll cells of nonacclimated and cold-acclimated spinach, Plant Physiol., 1991, vol. 97, no. 1, pp. 175181.

16. Pihakaski-Maunsbach, K. and Puhakainen, T., Effect of cold exposure on cortical microtubules of rye (Secale cereale) as observed by immunocytochemistry, Physiol. Plant, 1995, vol. 93, no. 3, pp. 563571.

17. Wang, Q.Y. and Nick, P., Cold acclimation can induce microtubular cold stability in a manner distinct from abscisic acid, Plant Cell Physiol., 2001, vol. 42, no. 9, pp. 9991005.

18. Bartolo, M.E. and Carter, J.V., Effect of microtubule stabilization on the freezing tolerance of mesophyll cells of spinach, Plant Physiol., 1991, vol. 97, pp. 182187.

19. Sakiyama, M. and Shibaoka, H., Effects of abscisic acid on the orientation and cold stability of cortical microtubules in epicotyl cells of the dwarf pea, Protoplasma, 1990, vol. 157, nos. 13, pp. 165171.

20. Kerr, G.P. and Carter, J.V., Tubulin isotypes in rye roots are altered during cold acclimation, Plant Physiol., 1990, vol. 93, pp. 8388.

21. Degand, H., Faber, A.M., Dauchot, N., Mingeot, D., Watillon, B., Van Cutsem, P., Morsomme, P., and Boutry, M., Proteomic analysis of chicory root identifies proteins typically involved in cold acclimation, Proteomics, 2009, vol. 9, no. 10, pp. 29032907.

22. Huang, Y., Jin, D., Lu, C., Lan, X., Qiao, P., Li, H., and Chen, Y., Proteomic responses associated with freezing tolerance in the callus of the Tibetan alpine plant Saussurea laniceps during cold acclimation, Plant Cell Tiss. Organ Cult., 2016, vol. 124, pp. 8195.

23. Ahad, A., Wolf, J., and Nick, P., Activation-tagged tobacco mutants that are tolerant to antimicrotubular herbicides are cross-resistant to chilling stress, Transgenic Res., 2003, vol. 12, pp. 615629.

24. Christov, N.K., Imai, R., and Blume, Y.B., Differential expression of two winter wheat alpha-tubulin genes during cold acclimation, Cell Biol. Int., 2008, vol. 32, no. 5, pp. 574578.

25. Farajalla, M. and Gulick, P.J., The alpha-tubulin gene family in wheat (Triticum aestivum L.) and differential gene expression during cold acclimation, Genome, 2007, vol. 50, pp. 502519.

26. Buy, D.D., Demkovich, A.E., Pirko, Ya.V., Korkhovoy, V.I., and Blume, Ya.B., Analysis of gene expression of TUBA-2-3 during cold acclimation in varieties of soft wheat Demetra and Elegiya, Naukovi Dopovidi NUBiP, 2015, vol. 8, no. 57.

27. Buy, D.D., Pirko, Ya.V., and Blume, Ya.B., Expression of winter and spring wheat alpha-tubulin gene S during cold acclimation, Factory Eksp. Evol. Org., 2015, vol. 17, pp. 2730.

28. Buy, D.D., Demkovich, A.E., Pirko, Ya.V., and Blume, Ya.B., Expression analysis of alpha-tubulin genes during cold acclimation in winter wheat Demetra, Faktori Eksp. Evol. Org., 2017, vol. 21, pp. 107111.

29. Paul, A., Lal, L., Ahuja, P.S., and Kumar, S., Alpha-tubulin (CsTUA) up-regulated during winter dormancy is a low temperature inducible gene in tea [Camellia sinensis (L.) O. Kuntze]. Mol. Biol. Rep., vol. 39, pp. 34853490. 30. Oakley, R., Wang, Y., Ramakrishna, W., Harding, S., and Tsai, C., Differential expansion and expression of alpha- and beta-tubulin gene families in Populus, Plant Physiol., 2007, vol. 145, no. 3, pp. 961973.

31. Nyporko, A.Y., Demchuk, O.N., and Blume, Y.B., Cold adaptation of plant microtubules: structural interpretation of primary sequence changes in a highly conserved region of alpha-tubulin, Cell Biol. Int., 2003, vol. 27, no. 3, pp. 241243.

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