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Expression analysis of α-tubulin genes during cold acclimation in winter and spring soft wheat
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SUMMARY. The expression profiles of 15 α-tubulin genes in spring and winter varieties of common wheat during cold acclimation were studied. Among the studied genes, two subfamilies were identified (3 genes in each) with elevated expression levels detected at the initial stages of cold acclimation. In particular, the Tuba-2-3 gene, which, within its subfamily, is characterized by the most pronounced amplitude of the initial expression level increase. Also the greatest differences in expression levels between varieties were found for this gene within its subfamily. In the case of winter varieties, higher values of expression levels for this tubulin gene were detected, that persist for a long time (up to the 7th day of acclimation). A significant initial increase in expression levels for all α-tubulin genes of the 4th subfamily was registered, reaching maximum values during further acclimation. The high initial values of the expression levels of this subfamily genes may also indicate their important role in resistance ensuring of wheat microtubules to low temperatures in the early stages of cold acclimation.
Key words: Triticum aestivum L., alpha-tubulin, gene expression, cold acclimation
E-mail: denisbuy90 gmail.com, blume.yaroslav nas.gov.ua; demand.dn gmail.com, yarvp1 gmail.com
1. Guo, X., Liu, D., and Chong, K., Cold signaling in plants: Insights into mechanisms and regulation, J. Integr. Plant Biol., 2018, vol. 60, no. 9, pp. 745Ц756. https://doi.org/10.1111/jipb.12706
2. Miura, K. and Furumoto, T., Cold signaling and cold response in plants, Int. J. Mol. Sci., 2013, vol. 14, no. 3, pp. 5312Ц5337. https://doi.org/10.3390/ijms14035312
3. Tardif, G., Kane, N.A., Adam, H., Labrie, L., Major, G., Gulick, P., Sarhan, F., and Lalibert, J.F., Interaction network of proteins associated with abiotic stress response and development in wheat, Plant Mol. Biol., 2007, vol. 63, no. 5, pp. 703Ц718. https://doi.org/10.1007/s11103-006-9119-6
4. Xiong, L., Schumaker, K.S., and Zhu, J.K., Cell signaling during cold, drought, and salt stress, Plant Cell, 2002, vol. 14, pp. 165Ц183. https://doi.org/10.1105/tpc.000596
5. Yadav, S.K., Cold stress tolerance mechanisms in plants. A review, Agron. Sustain. Dev., 2010, vol. 30, no. 3, pp. 515Ц527. https://doi.org/10.1051/agro/2009050
6. Orvar, B.L., Sangwan, V., Omann, F., and Dhindsa, R.S., Early steps in cold sensing by plant cells: the role of actin cytoskeleton and membrane fluidity, Plant J., 2000, vol. 23, no. 6, pp. 785Ц794.
7. Plohovska, S.H., Krasylenko, Y.A., and Yemets, A.I., Nitric oxide modulates actin filament organization in Arabidopsis thaliana primary root cells at low temperatures, Cell Biol. Int., 2018. https://doi.org/10.1002/cbin.10931 8. Plohovska, S.G., Yemets, A.I., and Blume, Ya.B., Influence of cold on organization of actin filaments of different types of root cells in Arabidopsis thaliana, Cytol. Genet., 2016, vol. 50, no. 5, pp. 318Ц323. https://doi.org/10.3103/s0095452716050108
9. Pokorna, J., Sites of actin filament initiation and reorganization in cold treated tobacco cells, J. Plant Cell Environ., 2004, vol. 27, no. 5, pp. 641Ц653. https://doi.org/10.1111/j.1365-3040.2004.01186.x
10. Abdrakhamanova, A., Wang, Q.Y., Khokhlova, L., and Nick, P., Is microtubule disassembly a trigger for cold acclimation?, Plant Cell Physiol., 2003, vol. 44, no. 7, pp. 676Ц686.
11. Kerr, G.P. and Carter, J.V., Relationship between freezing tolerance of root-tip cells and cold stability of microtubules in rye (Secale cereale L. cv. Puma), Plant Physiol., 1990, vol. 93, no. 1, pp. 77Ц82.
12. Sheremet, Y.A., Yemets, A.I., and Blume, Y.B., Inhibitors of tyrosine kinases and phosphatases as a tool for the investigation of microtubule role in plant cold response, Cytol. Genet., 2012, vol. 46, no. 1, pp. 1Ц8. https://doi.org/10.3103/S0095452712010112
13. Sproule, K., Microtubule involvement in the plant low temperature response, PhD Thesis, University of Saskatchewan, Saskatoon, 2008.
14. Jian, L.C., Sun, L.H., and Liu, Z.P., Studies on microtubule cold stability in relation to plant cold hardiness, Acta Bot. Sinica, 1989, vol. 31, pp. 737Ц741.
15. Bartolo, M.E. and Carter, J.V., Microtubules in the mesophyll cells of nonacclimated and cold-acclimated spinach, Plant Physiol., 1991, vol. 97, no. 1, pp. 175Ц181.
16. Pihakaski-Maunsbach, K. and Puhakainen, T., Effect of cold exposure on cortical microtubules of rye (Secale cereale) as observed by immunocytochemistry, Physiol. Plant, 1995, vol. 93, no. 3, pp. 563Ц571. https://doi.org/10.1111/j.1399-3054.1995.tb06859.x
17. Wang, Q.Y. and Nick, P., Cold acclimation can induce microtubular cold stability in a manner distinct from abscisic acid, Plant Cell Physiol., 2001, vol. 42, no. 9, pp. 999Ц1005.
18. Bartolo, M.E. and Carter, J.V., Effect of microtubule stabilization on the freezing tolerance of mesophyll cells of spinach, Plant Physiol., 1991, vol. 97, pp. 182Ц187.
19. Sakiyama, M. and Shibaoka, H., Effects of abscisic acid on the orientation and cold stability of cortical microtubules in epicotyl cells of the dwarf pea, Protoplasma, 1990, vol. 157, nos. 1Ц3, pp. 165Ц171.
20. Kerr, G.P. and Carter, J.V., Tubulin isotypes in rye roots are altered during cold acclimation, Plant Physiol., 1990, vol. 93, pp. 83Ц88.
21. Degand, H., Faber, A.M., Dauchot, N., Mingeot, D., Watillon, B., Van Cutsem, P., Morsomme, P., and Boutry, M., Proteomic analysis of chicory root identifies proteins typically involved in cold acclimation, Proteomics, 2009, vol. 9, no. 10, pp. 2903Ц2907. https://doi.org/10.1002/pmic.200800744
22. Huang, Y., Jin, D., Lu, C., Lan, X., Qiao, P., Li, H., and Chen, Y., Proteomic responses associated with freezing tolerance in the callus of the Tibetan alpine plant Saussurea laniceps during cold acclimation, Plant Cell Tiss. Organ Cult., 2016, vol. 124, pp. 81Ц95.
23. Ahad, A., Wolf, J., and Nick, P., Activation-tagged tobacco mutants that are tolerant to antimicrotubular herbicides are cross-resistant to chilling stress, Transgenic Res., 2003, vol. 12, pp. 615Ц629. https://doi.org/10.1023/A:1025814814823
24. Christov, N.K., Imai, R., and Blume, Y.B., Differential expression of two winter wheat alpha-tubulin genes during cold acclimation, Cell Biol. Int., 2008, vol. 32, no. 5, pp. 574Ц578.
25. Farajalla, M. and Gulick, P.J., The alpha-tubulin gene family in wheat (Triticum aestivum L.) and differential gene expression during cold acclimation, Genome, 2007, vol. 50, pp. 502Ц519.
26. Buy, D.D., Demkovich, A.E., Pirko, Ya.V., Korkhovoy, V.I., and Blume, Ya.B., Analysis of gene expression of TUBA-2-3 during cold acclimation in varieties of soft wheat Demetra and Elegiya, Naukovi Dopovidi NUBiP, 2015, vol. 8, no. 57.
27. Buy, D.D., Pirko, Ya.V., and Blume, Ya.B., Expression of winter and spring wheat alpha-tubulin gene S during cold acclimation, Factory Eksp. Evol. Org., 2015, vol. 17, pp. 27Ц30.
28. Buy, D.D., Demkovich, A.E., Pirko, Ya.V., and Blume, Ya.B., Expression analysis of alpha-tubulin genes during cold acclimation in winter wheat Demetra, Faktori Eksp. Evol. Org., 2017, vol. 21, pp. 107Ц111.
29. Paul, A., Lal, L., Ahuja, P.S., and Kumar, S., Alpha-tubulin (CsTUA) up-regulated during winter dormancy is a low temperature inducible gene in tea [Camellia sinensis (L.) O. Kuntze]. Mol. Biol. Rep., vol. 39, pp. 3485Ц3490. https://doi.org/10.1007/s11033-011-1121-7 30. Oakley, R., Wang, Y., Ramakrishna, W., Harding, S., and Tsai, C., Differential expansion and expression of alpha- and beta-tubulin gene families in Populus, Plant Physiol., 2007, vol. 145, no. 3, pp. 961Ц973. https://doi.org/10.1104/pp.107.107086
31. Nyporko, A.Y., Demchuk, O.N., and Blume, Y.B., Cold adaptation of plant microtubules: structural interpretation of primary sequence changes in a highly conserved region of alpha-tubulin, Cell Biol. Int., 2003, vol. 27, no. 3, pp. 241Ц243. https://doi.org/10.1016/S1065-6995(02)00342-6
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