SUMMARY. Genetic transformation of inbred lines and maize hybrids F1, registered in Ukraine, has been carried out. The study used a biolistic method for the genetic transformation of maize immature embryos that formed callus tissue, pAHC25 vector containing the genes of phosphinothricin-N-acetyltransferase (bar) and β-glucuronidase (uidA). As a result of the transformation of callus tissue of maize genotypes, resistant to phosphinothricin lines and regenerated plants were obtained. The activity of β-glucuronidase in herbicide-resistant calli was detected. The presence of bar gene in the calli DNA was demonstrated by PCR method. The rate of stable transformation ranged from 2,2 to 30 % depending on genotype. The relationship of transient expression results of β-glucuronidase gene and stable genetic transformation was observed. The proposed protocol for the genetic transformation of maize using a transient expression study of β-glucuronidase gene allows for a significant simplification of selection of competent to genetic transformation genotypes and creation of transgenic organisms with new traits.
Keywords: Zea mays L., immature embryos, biolistic genetic transformation, bar, uidA
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