Zygotic autopoliploidization of rye (Secale cereale L.)

Gordej I.S., Lyusikov O.M., Gordej I.A.

SUMMARY. The paper presents the results of zygotic autopoly-ploidization by the nitrous oxide (N₂O) of diploid cultivars and F₁ hybrids of winter rye. It has been shown that the method of creating zygotic rye tetraploids by the nitrous oxide (N₂O) is effective and allows obtaining up to 86,0 % of tetraploids. The average yield of tetraploids was 51,0 %. By this method we created 9 new rye tetraploids. The created tetraploids are characterized by chromosomal balance and low levels of aneuploidy (up to 9,7 %). Meiosis in rye zygotic tetraploids occurs with significantly more defections (P ≤ 0,05) than in the original diploids. It has been established that in the created tetraploids, part of the nuclear DNA is eliminated to the C₆–C₇ generations. On the basis of the obtained tetraploid Yubileinaya, a new variety of winter tetraploid rye Camea 16 was created.

Key words: winter rye, nitrous oxide, diploids, zygotic tetraploids, meiosis, DNA elimination

Tsitologiya i Genetika 2019, vol. 53, no. 5, pp. 13-19

E-mail: I_Gordej777 mail.ru

Gordej I.S., Lyusikov O.M., Gordej I.A. Zygotic autopoliploidization of rye (Secale cereale L.), Tsitol Genet., 2019, vol. 53, no. 5, pp. 13-19.

In "Cytology and Genetics":
I. S. Gordej, O. M. Lyusikov, I. A. Gordej Zygotic Autopolyploidization of Rye (Secale cereale L.), Cytol Genet., 2019, vol. 53, no. 5, pp. 357–362
DOI: 10.3103/S0095452719050086

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