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Zygotic autopoliploidization of rye (Secale cereale L.)

Gordej I.S., Lyusikov O.M., Gordej I.A.


SUMMARY. The paper presents the results of zygotic autopoly-ploidization by the nitrous oxide (N2O) of diploid cultivars and F1 hybrids of winter rye. It has been shown that the method of creating zygotic rye tetraploids by the nitrous oxide (N2O) is effective and allows obtaining up to 86,0 % of tetraploids. The average yield of tetraploids was 51,0 %. By this method we created 9 new rye tetraploids. The created tetraploids are characterized by chromosomal balance and low levels of aneuploidy (up to 9,7 %). Meiosis in rye zygotic tetraploids occurs with significantly more defections (P ≤ 0,05) than in the original diploids. It has been established that in the created tetraploids, part of the nuclear DNA is eliminated to the C6C7 generations. On the basis of the obtained tetraploid Yubileinaya, a new variety of winter tetraploid rye Camea 16 was created.

Key words: winter rye, nitrous oxide, diploids, zygotic tetraploids, meiosis, DNA elimination

Tsitologiya i Genetika 2019, vol. 53, no. 5, pp. 13-19

E-mail: I_Gordej777

Gordej I.S., Lyusikov O.M., Gordej I.A. Zygotic autopoliploidization of rye (Secale cereale L.), Tsitol Genet., 2019, vol. 53, no. 5, pp. 13-19.

In "Cytology and Genetics":
I. S. Gordej, O. M. Lyusikov, I. A. Gordej Zygotic Autopolyploidization of Rye (Secale cereale L.), Cytol Genet., 2019, vol. 53, no. 5, pp. 357362
DOI: 10.3103/S0095452719050086


1. Schlegel, R., Hybrid breeding boosted molecular genetics in rye, Vavilov J. Genet. Breed., 2015, vol. 19, no. 5, pp. 589603.

2. Privalov, F.I. and Urban, E.P., Achievements and problems of high yield crops breeding in the republic of Belarus, Proc. Natl. Acad. Sci. Belarus, Ser. Agr. Sci., 2016, no. 3, pp. 4149.

3. Pfahler, P.L., Barnett, R.D., and Luke, H.H., Diploid-tetraploid comparisons in rye. IV. Grain production, Crop Sci., 1987, vol. 27, no. 3, pp. 431435.

4. Gordej, I.S., Structural changes of rye genome after zygotic duplication, Mol. Appl. Genet., 2016, vol. 21, pp. 3745.

5. Lundqvist, A., Heterosis and inbreeding depression in autotetraploid rye, Heredity, 1966, vol. 56, nos. 2/3, pp. 317366.

6. Dorsey, E., Induced polyploidy in wheat and rye: chromosome doubling in Triticum, Secale and TriticumSecale hybrids produced by temperature changes, J. Hered., 1936, vol. 27, no. 4, pp. 155160.

7. Marasek-Ciolakowska, A., Nishikawa, T., Shea, D., and Okazaki, K., Breeding of lilies and tulips, Interspecific hybridization and genetic background, Breed Sci., 2018, vol. 68, no. 1, pp. 3552.

8. Berdahl, J. and Barker, R., Characterization of autotetraploid Russian wild rye produced with nitrous oxide, Crop Sci., 1991, vol. 31, no. 5, pp. 11531155.

9. Chen, Z. and Ni, Z., Mechanisms of genomic rearrangements and gene expression changes in plant polyploids, BioEssays, 2006, vol. 28, no. 3, pp. 204252.

10. Liu, B., Xu, C., Zhao, N., Qi, B., Kimatu, J., Pang, J., and Han, F., Rapid genomic changes in polyploid wheat and related species: implications for genome evolution and genetic improvement, J. Genet. Genomics, 2009, vol. 36, no. 9, pp. 519528.

11. Lavergne, S., Muenke, N., and Molofsky, J., Genome size reduction can trigger rapid phenotypic evolution in invasive plants, Ann. Bot., 2010, vol. 105, pp. 109116.

12. Adams, K., Polyploidy and genome evolution in plants, Curr. Opin. Plant Biol., 2005, vol. 8, no. 2, pp. 135141.

13. Belko, N.B., Gordej, I.A., Shchetko, I.S., and Gordej, I.S., Creating tetraploid forms of winter rye using nitrous oxide and the genetic effects of genome duplication, Fact. Exp. Evol. Organisms, 2011, vol. 10, pp. 1419.

14. Gaaliche, B., Majdoub, A., Trad, M., and Mars, M., Assessment of pollen viability, germination, and tube growth in eight Tunisian caprifig (Ficus carica L.) cultivars, Int. Schol. Res. Not., ISRN Agronomy, 2013.

15. Jellen, E., C-banding of plant chromosomes, Methods Mol. Biol., 2016, vol. 1429, pp. 15.

16. Pichugin, Yu.G., Semyanov, K.A., Chernyshev, A.V., Palchikova, I.G., Omelyanchuk, L.V., and Maltsev, V.P., Nucleus DNA content measurement methods features, Cytology, 2012, vol. 54, no. 2, pp. 185190.

17. Bai, C., Alverson, W., Follansbee, A., and Waller, D., New reports of nuclear DNA content for 407 vascular plant taxa from the United States, Ann. Bot., 2012, vol. 110, no. 8, pp. 16231629.

18. McCleery, R., Watt, T., and Hart, T., Introduction to Statistics for Biology, Chapman and Hall/CRC, 2007, 3rd ed.

19. Kitamura, S., Mechanism of action of nitrous oxide gas applied as a polyploidizing agent during meiosis in lilies, Sex. Plant Reprod., 2009, vol. 22, no. 1, pp. 914.

20. Leitch, I. and Bennet, M., Genome downsizing in polyploidy plants, Biol. J. Linn. Soc., 2004, vol. 82, no. 4, pp. 651663.

21. Bennett, M., Bhandol, P., and Leitch, I., Nuclear DNA amounts in angiosperms and their modern uses807 new estimates, Ann. Bot., 2000, vol. 86, no. 4, pp. 859909.

22. Raina, S., Parida, A., Koul, K., Salimath, S., Bisht, M., Raja, V., and Khoshoo, T., Associated chromosomal DNA changes in polyploids, Genome, 1994, vol. 37, no. 4, pp. 560564.

23. Martelotto, L., Ortiz, J., Juliana, S., and Francisco, E., Genome rearrangements derived from autopolyploidization in Paspalum sp., Plant Sci., 2007, vol. 172, no. 5, pp. 970977.

24. Ozkan, H., Levy, M., and Feldman, A., Allopolyploidy-induced rapid genome evolution in the wheat (AegilopsTriticum), Plant Cell, 2001, vol. 13, no. 8, pp. 17351747.

25. Gustafson, J. and Bennett, M., The effect of telomeric heterochromatin from Secale cereale on Triticale (Triticosecale). I. The influence of the loss of several blocks of telomeric heterochromatin on early endosperm development and kernel characteristics at maturity, Genome, 2011, vol. 24, no. 1, pp. 8392.

26. Devos, K., Brown, J., and Bennetzen, J., Genome size reduction through illegitimate recombination counteracts genome expansion in Arabidopsis, Genome Res., vol. 12, no. 7, pp. 10751079.

27. Kravets, E.A., Sidorchuk, Yu.V., Horyunova, I.I., Plohovskaya, S.H., Mursalimov, S.R., Deineko, E.V., Yemets, A.I., and Blume, Ya.B., Intra- and intertissular cytomictic interactions in the microsporogenesis of mono- and dicotyledonous plants, Cytol. Genet., 2016, vol. 50, no. 5, pp. 316.

28. Butrille, D. and Boiteux, L., Selection-mutation balance in polysomic tetraploids: Impact of double reduction and gametophytic selection on the frequency and subchromosomal localization of deleterious mutation, Proc. Natl. Acad. Sci. U. S. A., 2000, vol. 97, no. 12, pp. 660866013.

29. Kunakh, V.A., Ontogenetic plasticity of the genome as the basis for plant adaptability, in Zhebrakovs Readings III Transformation of Genomes, Minsk: Pravo i economica, 2011.

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