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Effect of 1-34 fragment of parathyroid hormone on differentiation of chondroprogenitor cells and regeneration of arthrodial cartilage
SUMMARY. This study was aimed at investigating the effect of periodic and continuous administration of 1-34 fragment of parathyroid hormone (PTH) on the diffe-rentiation of bovine prechondroblasts maintained in chondrogenic and osteogenic media. In addition, the regeneration of cartilage tissue of outbred rats was studied in terms of the dependence of the mentioned process on the level of parathyroid hormone and the changes in subchondral bone. Monolayer cultures of bovine chondroprogenitor cells, maintained in osteogenic and chondrogenic media, were studied with periodic and continuous administration of 1-34 PTH with subsequent immunofluorescent analysis of relevant markers. Knee joints were also surgically operated to form the deficiency of arthrodial cartilage with further investigation of regeneration processes depending on the administration of PTH in vivo via histochemical analysis of surgically operated knee joints of rats. The data obtained testify to the modulating effect of PTH on chondrogenic differentiation of stem cells and therapeutic potential of this hormone for the restoration of cartilage tissue.
Key words: parathyroid hormone, chondrocytes, stem cells, differentiation
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