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Frequency of spontaneous and radiation-induced chromosome aberrations in peripheral blood lymphocytes of persons of different age

Shemetun O.V., Talan O.A., Demchenko O.M., Kurinniy D.A., Papuga M.S., Pilinska M.A.

 




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SUMMARY. Using the method of GTG staining of human metapha-se chromosomes, the frequency of spontaneous and induced by X-ray in vitro (0,25 Gy) chromosome aberrations as well as the level of chromosomal instability as a result of the bystander effect in the blood lymphocytes of individuals aged from 12 to 102 years had been investigated. The average group frequency of spontaneous chromosome aberrations in adolescents (1216 years), middle-aged people (3352 years) and centenarians (90102 years) was identical (p > 0,05), whereas in elderly persons (6070 years) it was higher due to increase of chromatid type aberrations rate
(p <0,05). In the irradiated in vitro blood lymphocytes of individuals aged 1216 years, 3352 years and 90102 years the levels of chromosome aberrations did not differ among themselves (p > 0,05), however in persons aged 6070 years the total frequency of chromosomal aberrations exceeded the value of such indicators in other age groups due to the chromosome type aberrations (p < 0,05). In unexposed blood lymphocytes of ado-lescents, middle-aged and elderly persons, when co-cultivated with irradiated in vitro (0,25 Gy) cells, the induction of the bystander effect had been recorded. In lymphocytes of centenarians the development of the bystander effect had not been detected.

Tsitologiya i Genetika 2018, vol. 52, no. 6, pp. 86-92

E-mail: shemetun ukr.net, okstal ukr.net, _demchenko ukr.net, kurinnyi.d gmail.com, pmiroslav ukr.net, pww ukr.net

Shemetun O.V., Talan O.A., Demchenko O.M., Kurinniy D.A., Papuga M.S., Pilinska M.A. Frequency of spontaneous and radiation-induced chromosome aberrations in peripheral blood lymphocytes of persons of different age, Tsitol Genet., 2018, vol. 52, no. 6, pp. 86-92.

In "Cytology and Genetics":
O. V. Shemetun, O. A. Talan, O. M. Demchenko, D. A. Kurinnyi, M. S. Papuga, M. A. Pilinska Frequency of Spontaneous and Radiation-Induced Chromosomal Aberrations in Peripheral Blood Lymphocytes of Individuals of Different Ages, Cytol Genet., 2018, vol. 52, no. 6, pp. 461466
DOI: 10.3103/S0095452718060117


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