ISSN 0564-3783  

Main page
Information to authors
Editorial board
Mobile version

In Ukrainian

Export citations

Frequency of spontaneous and radiation-induced chromosome aberrations in peripheral blood lymphocytes of persons of different age

Shemetun O.V., Talan O.A., Demchenko O.M., Kurinniy D.A., Papuga M.S., Pilinska M.A.


Key words:

SUMMARY. Using the method of GTG staining of human metapha-se chromosomes, the frequency of spontaneous and induced by X-ray in vitro (0,25 Gy) chromosome aberrations as well as the level of chromosomal instability as a result of the bystander effect in the blood lymphocytes of individuals aged from 12 to 102 years had been investigated. The average group frequency of spontaneous chromosome aberrations in adolescents (1216 years), middle-aged people (3352 years) and centenarians (90102 years) was identical (p > 0,05), whereas in elderly persons (6070 years) it was higher due to increase of chromatid type aberrations rate
(p <0,05). In the irradiated in vitro blood lymphocytes of individuals aged 1216 years, 3352 years and 90102 years the levels of chromosome aberrations did not differ among themselves (p > 0,05), however in persons aged 6070 years the total frequency of chromosomal aberrations exceeded the value of such indicators in other age groups due to the chromosome type aberrations (p < 0,05). In unexposed blood lymphocytes of ado-lescents, middle-aged and elderly persons, when co-cultivated with irradiated in vitro (0,25 Gy) cells, the induction of the bystander effect had been recorded. In lymphocytes of centenarians the development of the bystander effect had not been detected.

Tsitologiya i Genetika 2018, vol. 52, no. 6, pp. 86-92

E-mail: shemetun, okstal, _demchenko, kurinnyi.d, pmiroslav, pww

Shemetun O.V., Talan O.A., Demchenko O.M., Kurinniy D.A., Papuga M.S., Pilinska M.A. Frequency of spontaneous and radiation-induced chromosome aberrations in peripheral blood lymphocytes of persons of different age, Tsitol Genet., 2018, vol. 52, no. 6, pp. 86-92.

In "Cytology and Genetics":
O. V. Shemetun, O. A. Talan, O. M. Demchenko, D. A. Kurinnyi, M. S. Papuga, M. A. Pilinska Frequency of Spontaneous and Radiation-Induced Chromosomal Aberrations in Peripheral Blood Lymphocytes of Individuals of Different Ages, Cytol Genet., 2018, vol. 52, no. 6, pp. 461466
DOI: 10.3103/S0095452718060117


1. Kašuba, V., Rozgaj, R., and Jazbec, A., Chromosome aberration in peripheral blood lymphocytes of Croatian hospital staff occupationally exposed to low levels of ionising radiation, Arh. Hig. Rada Toksikol., 2008, vol. 59, no. 4, pp. 251259. doi 10.2478/10004-1254-59-2008-1909

2. Pilinskaya, M.A., Shemetun, G.M., Shemetun, O.V., Dybskyi, S.S., Dybska, O.B., Talan, O.O., Pedan, L.R., and Kurinnyi, D.A., Chromosomal mutagenesis in human somatic cells: 30-year cytogenetic monitoring after Chornobyl accident, Exp. Oncol., 2016, vol. 38, no. 4, pp. 276279.

3. Tawn, E.J., Curwen, G.B., Jonas, P., Gillies, M., Hodgsonb, L., and Cadwell, K.K., Chromosome aberations determined by FISH in radiation workers from the Sellafield Nuclear Facility, Radiat. Res., 2015, vol. 184, no. 3, pp. 296303. doi 10.1667/RR14125.1

4. Nugis, V.Yu. and Kozlova, M.G., Cytogenetic examination of persons working in the area of radiation accident at the Fukushima-1 NPP in Japan, Saratov J. Med. Sci. Res., 2014, vol. 10, no. 4, pp. 796800.

5. Marković, S.Z., Nikolić, L.I., Hamidović, J.Lj., Grubor, M.G., Grubor, M.M., and Kastratović, D.A., Chromosomes aberrations and environmental factors, Hosp. Pharmacol., 2017, vol. 4, no. 1, pp. 486490. doi 10.5937/hpimj1701486M

6. Ryu, T.H., Kim, J.H., and Kim, J.K., Chromosomal aberrations in human peripheral blood lymphocytes after exposure to ionizing radiation, Genome Integr., 2016, vol. 7, no. 5, pp. 13. doi 10.4103/2041-9414.197172

7. Shemetun, O.V. and Pilinska, M.A., Radiation-induced bystander effect, Cytol. Genet., 2007, vol. 41, no. 4, pp. 251255. doi 10.3103/S0095452707040111

8. Shemetun, O.V., Talan, O.O., and Pilinska, M.A., Cytogenetic characteristics of the radiation-induced bystander effect and its persistence in human blood lymphocytes, Cytol. Genet., 2014, vol. 48, no. 4, pp. 244249. doi 10.3103/S0095452714040069

9. Shemetun, O.V. and Talan, O.O., Research of oxidative stress participation in the development of radiation-induced bystander effect in human peripheral blood lymphocytes, Dopov. Nac. Akad. Nauk Ukr., 2014, no. 8, pp. 144148. org/ doi 10.15407/dopovidi2014.08.144

10. Crouch, J.D. and Brosh, R.M., Mechanistic and biological considerations of oxidatively damaged DNA for helicase-dependent pathways of nucleic acid metabolism, Free Radic. Biol. Med., 2017, vol. 107, pp. 245257. doi 10.1016/j.freeradbiomed.2016.11.022

11. Von Zglinicki, T., Oxidative stress shortens telomeres, Trends Biochem. Sci., 2002, vol. 27, no. 7, pp. 339344.

12. Itri, R., Junqueira, C.H., Mertins, O., and Baptista, S.M., Membrane changes under oxidative stress: the impact of oxidized lipids, Biophys. Rev., 2014, vol. 6, no. 1, pp. 4761. doi 10.1007/s12551-013-0128-9

13. Lien Ai Pham-Huy, Hua He, and Chuong Pham-Huy, Free radicals, antioxidants in disease and health, Int. J. Biomed. Sci., 2008, vol. 4, no. 2, pp. 8996.

14. Lombard, D.B., Chua, K.F., Mostoslavsky, R., Franco, S., Gostissa, M., and Alt, F.W., DNA repair, genome stability, and aging, Cell, 2005, vol. 120, no. 4, pp. 497512. doi 10.1016/j.cell.2005.01.028

15. Mei-Ren Pan, Kaiyi Li, Shiaw-Yih Lin, and Wen-Chun Hung, Connecting the dots: from DNA damage and repair to aging, Int. J. Mol. Sci., 2016, vol. 17, no. 5, p. 685. doi 10.3390/ijms17050685

16. Little, J.B., Genomic instability and radiation, J. Radiol. Prot., 2002, vol. 23, no. 2, pp. 173181.

17. Valko, M., Rhodes, C.J., Moncol, J., Izakovic, M., and Mazur, M., Free radicals, metals and antioxidants in oxidative stress-induced cancer, Chem. Biol. Interact., 2006, vol. 160, no. 1, pp. 140. doi 10.1016/j.cbi.2005.12.009

18. Joksic, G., Petrovic, S., and Ilic, Z., Age-related changes in radiation-induced micronuclei among healthy adults, Braz. J. Med. Biol. Res., 2004, vol. 37, no. 8, pp. 11111117. org/doi 10.1590/S0100-879X2004000800002

19. Sevankaev, A.V., Khvostunov, I.K. and Potetnia, V.I., The low dose and low dose rate cytogenetic effects induced by gamma-radiation in human blood lymphocytes in vitro. II. The results of cytogenetic study, Rad. Biol. Radioecol., 2012, vol. 52, no. 1, pp. 1124.

20. Gricienė, B. and Slapšytė, G., Assessment of chromosomal aberrations in workers chronically exposed to ionizing radiation, Biologija, 2007, vol. 53, no. 4, pp. 510.

21. El-Benhawy, S.A., Sadek, N.A., Behery, A.K., Issa, N.M., and Ali, O.K., Chromosomal aberrations and oxidative DNA adduct 8-hydroxy-2-deoxyguanosine as biomarkers of radiotoxicity in radiation workers, J. Radiat. Res. Appl. Sci., 2016, vol. 9, no. 3, pp. 249258.

22. Bochkov, N.P., Chebotarev, A.N., Katosova, L.D., and Platonova, V.I., The Database for analysis of quantitative characteristics of chromosome aberration frequencies in the culture of human peripheral blood lymphocytes, Russ. J. Genet., 2001, vol. 37, no. 4, pp. 440447.

23. Sirota, N.P. and Kuznetsova, E.A., Spontaneous DNA damage in peripheral blood leukocytes from donors of different age, Bull. Exp. Boil. Med., 2008, vol. 145, no. 2, pp. 194197.

24. Lyubimova, N.E. and Vorobtsova, I.E., The effect of age and low-dose irradiation on the chromosomal aberration frequency in human lymphocytes, Rad. Biol. Radioecol., 2007, vol. 47, no. 1, pp. 8085.

25. Ahmad, O.B., Boschi-Pinto, C., Christopher, A.D., Murray, J.L., and Lozano, R.L., Age Standardization of Rates: A New WHO Standard, GPE Discussion Paper Series: No.31 EIP/GPE/EBD World Health Organization, 2001.

26. Zirova-Lubimova, T.E. and Horovenko, N.H., Cytogenetic Methods for Studying Human Chromosomes: Guidelines, Kyiv, 2003.

27. ISCN (2013): An International System for Human Cytogenetic Nomenclature, Shaffer, L.G., McGowan-Jordan, J., and Schmid, M., Eds., Basel: S. Karger, 2013.

28. Rosner, B., Fundamentals of Biostatistics, Cengage Learning, 2015, 8th ed.

29. Erceg, P., Milosevic, D.P., Despotovic, N., and Davidovic, M., Chromosomal changes in ageing, J. Genetics, 2007, vol. 86, no. 3, pp. 2778.

30. Wojda, A., Zietkiewicz, E., Mossakowska, M., Pawlowski, W., Skrzypczak, K., and Witt, M., Correlation between the level of cytogenetic aberrations in cultured human lymphocytes and the age and gender of donors, J. Gerontol. A Biol. Sci. Med. Sci., 2006, vol. 61, no. 8, pp. 763772.

31. Vorobtsova, I.E., Kanaeva, A.I., Petrova, I.A., Semenov, A.V., Pleskach, N.M., Spivak, I.M., Timo-nina, G.A., Prokofeva, V.V., Iartseva, N.M., and Mikhelson, V.M., Age dynamics of stable chromosome aberration frequency in humans with natural and pathological senescence, Tsitologiia, 2004, vol. 46, no. 12, pp. 10301034.

32. Vorobtsova, I.E. and Semenov, A.V., The age dynamics of spontaneous and induced in vitro chromosome aberrations in human lymphocytes under natural and radiation induced senescence, Rad. Biol. Radioecol., 2010, vol. 50, no. 3, pp. 253258.

33. Repina, L.A., Cytogenetic effects of low doses of accelerated charged particles in human blood lymphocytes in vitro, Rad. Biol. Radioecol., 2006, vol. 46, no. 4, pp. 461465.

34. Payne, B.A. and Chinnery, P.F., Mitochondrial dysfunction in aging: much progress but many unresolved questions, Biochim. Biophys. Acta, 2015, vol. 1847, no. 11, pp. 13471353. doi 10.1016/j.bbabio.2015.05.022

35. Vasylenko, O.P., Pronina, O.V., and Rushkovsky, S.R., Bystander effect in human lymphocytes incubated with irradiated mitochondrial DNA deficient yeast cells, Radioprotection, 2011, vol. 46, no. 6, pp. 555559. org/ doi 10.1051/radiopro/20116908s

Copyright© ICBGE 2002-2021 Coded & Designed by Volodymyr Duplij Modified 25.07.21