Tube formation potential of BMSCs and USSCs in response to HIF-1α overexpression under hypoxia

Khajeh S., Mostafavi-Pour Z., Alaee S., Soleimani M., Razban V.

Despite numerous studies on therapeutic effects of mesenchymal stem cells on ischemic tissue regeneration, including angiogenesis, their mechanism of action remains ambiguous. Due to the scarce of investigations based on different stem cell sources with known inherent molecular differences, present study compare tube formation of Bone marrow Mesenchymal Stem Cells and Unrestricted Somatic Stem Cells with known reported different Hox gene expression profile in response to HIF-1α overexpression under hypoxia. This might shed light on some parameters for selection of more responsive source with improved therapeutic effects. Superior in vitro tube formation on Matrigel substratum has been observed by Unrestricted Somatic Stem Cells compared to Bone marrow Mesenchymal Stem Cells which might possibly be due to the discriminating molecular properties of stem cell sources. It and may help choosing the appropriate stem cell type for a given therapeutic expectations and also suggest some potential targets for future genetic modification of stem cells.

Key words: Tube formation, Mesenchymal stem cell, Hy-poxia, HIF-1α

Tsitologiya i Genetika 2018, vol. 52, no. 3, pp. 78-80

• Department of Clinical Biochemistry, Medical school, Shiraz University of Medical Sciences, Shiraz, Iran
• Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
• Department of Hematology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
• Department of Molecular Medicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
• Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

E-mail: razban_vahid yahoo.com

Khajeh S., Mostafavi-Pour Z., Alaee S., Soleimani M., Razban V. Tube formation potential of BMSCs and USSCs in response to HIF-1α overexpression under hypoxia, Tsitol Genet., 2018, vol. 52, no. 3, pp. 78-80.

In "Cytology and Genetics":
V. Razban, S. Khajeh, S. Alaee, Z. Mostafavi-Pour, M. Soleimani Tube Formation Potential of BMSCs and USSCs in Response to HIF-1α Overexpression under Hypoxia, Cytol Genet., 2018, vol. 52, no. 3, pp. 236–244
DOI: 10.3103/S0095452718030064