TSitologiya i Genetika 2018, vol. 52, no. 3, 72-75
Cytology and Genetics 2018, vol. 52, no. 3, 222–230, doi: https://www.doi.org/10.3103/S009545271803012X

Genetic diversity of persian ecotypes of INDIAN WALNUT (Aeluropus littoralis (Gouan) Parl.) by AFLP and ISSR markers

Vafadar Shamasbi F., Nasiri N., Shokr E.

  • Department of Agricultural Biotechnology, Maragheh Branch, Islamic Azad University, Maragheh, Iran
  • Departmnet of Plant Breeding and Biotechnology, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
  • Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Iran
  • Genetics & Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran

Indian walnut Aeluropus littoralis (Gouan) Parl. well known for its salt tolerance and forage quality, is a perennial monocot grass from Chlorideae tribe that shows a high potential to become an important genetic model. Although, Aeluropus germplasm is very rich in Iran; but is still unexplored. In this study, 20 ecotypes of A. littoralis were gathered from different geographic zones and a set 16 primers (ISSRs-AFLPs combined) was used for genetic diversity evaluation. A total of 635 fragments and 594 polymorphic fragments were produced (93.5 %) and modest levels of genetic similarity were found (ranging from 0.38 to 0.71) among ecotypes. The results showed an equal competition between the two markers on po-lymorphism detection, so both molecular techniques were able to distinguish the A. littoralis ecotypes. Furthermore, dendrogram based on both the Bayesian and PCoA analysis showed a clear discrimination and significant variation among ecotypes; however, molecular clustering indicated a weak concordance with geographical grouping. The amount of molecular diversity revealed by molecular markers for A. littoralis in this study can be exploited for pasture conservation programs and new forage crop development.

Keywords: Aeluropus littoralis, Germplasm, ISSRs-AFLPs, Molecular diversity, Molecular markers

TSitologiya i Genetika
2018, vol. 52, no. 3, 72-75

Current Issue
Cytology and Genetics
2018, vol. 52, no. 3, 222–230,
doi: 10.3103/S009545271803012X

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